Fatty acid profiles of ?Pasteurella? piscicida: comparison with other fish pathogenic gram-negative bacteria

Abstract

The fatty acid methyl ester (FAME) profiles of "Pasteurella" piscicida were determined by gas chromatography and subjected to numerical analysis in comparison with those obtained for Vibrio anguillarum, Aeromonas salmonicida and Pasteurella species of clinical origin. The bacterial species studied shared important characteristics with respect to their FAME content: in all of them the saturated and unsaturated fatty acids of 16 carbon atoms were the predominant fatty acids. However, distinguishing features could be detected for each pathogen. Using either single linkage or complete linkage algorithms, strains were divided into four phena that corresponded to the different species, but showed a high degree of correlation among them. Although single linkage discriminated strains better within each phenum, complete linkage was more useful to establish the relationships among clusters. The results obtained support the idea that "Pasteurella" piscicida is related to members of the genera Vibrio and Aeromonas and indicate the need for exhaustive genetic studies to clarify the taxonomic position of this fish pathogen.

Isolation and characterization of the causative agent of pasteurellosis, Photobacterium damsela ssp. piscicida, from sole, Solea senegalensis (Kaup)

“…All the strains tested showed cross‐reactivity against all the antisera used. In contrast, no agglutination was recorded with other related fish‐pathogenic strains of the genera Vibrio and Aeromonas ( Romalde, Magari·os, Turnbull, Baya, Barja & Toranzo 1995).…”

Photobacterium damselae subsp. piscicida: an integrated view of a bacterial fish pathogen

“…It has been reported that P. piscicida shares major phenotypic characteristics with other fish pathogens including Vibrio anguillarum, Aeromonas salmonicida, and the clinically important pasteurellae, Pasteurella multocida and Pasteurella haemolytica [24]. A study of the fatty acid methyl ester (FAME) profiles by gas chromatography [44] revealed significant homogeneity in the major fatty acids among the different P. piscicida strains. In addition, comparison of these results with those obtained from representative strains of V. anguillarum, A. salmonicida and Pasteurella species of clinical origin suggested that the agent of fish pasteurellosis should be closer to Pasteurella than to Vibrio or Aeromonas.…”

Molecular diversity of Photobacterium damselae ssp. piscicida from cultured amberjacks (Seriola spp.) in Japan by pulsed-field gel electrophoresis and plasmid profiles

“…piscicida strains exhibited the same morphological, biochemical and physiological characteristics. And although some variation was observed in plasmid profiling and antimicrobial resistances (Toranzo et al 1983;Magariños et al 1992;Kim and Aoki 1993a), serotyping and lipopolysaccharide, fatty acid methyl ester and outer-membrane protein profiling also demonstrated the homogeneity of this bacterum (Magariños et al 1992;Romalde et al 1995;Romalde and Magariños 1997). Recently, with the development of DNA fingerprinting techniques, new approaches have become available and have been applied to P. damselae ssp.…”