Fatty acid oxidation capacity and fatty acid-binding protein content of different cell types isolated from rat heart

Linssen, M. C. J. G.; Vork, M; Jong, Y. F. De; Glatz, Jan F. C.; Vusse, Ger J.

doi:10.1007/978-1-4615-3936-0_3

Cited by 8 publications

(12 citation statements)

References 19 publications

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“…[4] Although minor quantities of fatty acid binding proteins (FABP) are present at this site, their role in transmembrane FA transport is assumed to be unimportant. [23] Once in the interstitial space, albumin will bind the FA for transport to the muscle cell membrane. [15,21] Here the FA are taken over by an FA-transporting protein, [24] or will cross the membrane directly because of their lipophilic nature.…”

Section: Factors Limiting Fatty Acid Uptake By Muscle Cellsmentioning

confidence: 99%

Strategies to Enhance Fat Utilisation During Exercise

Hawley

Brouns²,

Jeukendrup³

1998

Sports Medicine

101

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Section: Factors Limiting Fatty Acid Uptake By Muscle Cellsmentioning

confidence: 99%

Strategies to Enhance Fat Utilisation During Exercise

Hawley

Brouns²,

Jeukendrup³

1998

Sports Medicine

101

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“…In addition to the albumin in the interstitial space, there is a high level of cytoplasmic fatty acid-binding protein in the heart muscle cells and a low level of this protein in cultured endothelial cells. 26 The present studies now demonstrate that, for the rapid and efficient uptake of fatty acids by the heart, the capillary endothelial cells are of primary importance. To A model analysis of the dilution curves was carried out to document both the site of this effect and its magnitude.…”

Section: Discussionmentioning

confidence: 50%

The capillary transport system for free fatty acids in the heart.

Goresky

Stremmel

Rose

et al. 1994

Circulation Research

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The nature of the process by which free fatty acids, which are tightly bound to albumin, traverse the endothelium of cardiac capillaries to reach the cardiac muscle cells, so that they are extracted to a net extent of =40O%, needs clarification. Previous provided reassurance that the inferences arising from the modeling analysis were likely correct.Labeled albumin in plasma is exchanged with that present in the cardiac interstitium at a rate several orders of magnitude slower than the rate at which free fatty acid enters the interstitium7; its exchange during a single passage through the heart is too small to be measurable. The passage of free fatty acid from plasma (where it is bound to albumin) to the interstitium therefore occurs in a fashion independent of the exchange of albumin.

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“…The fatty acid is separated from albumin at the endothelial membrane, perhaps via special receptor/transporter sites, 36 transferred across the membrane and released into the cytosol of endothelial cells where it is complexed with another binding protein, fatty-acid binding protein (FABP). Although we once thought that there was no significant concentration of fatty-acid binding protein in cardiac endothelial cells, 18 more recent evidence for it is clear. 1 , 19 Transport via clefts between endothelial cells is thought to be negligible because albumin cannot pass through the clefts and the fraction of fatty acid which is free is so small.4 Next, the fatty acid is transferred across the abluminal endothelial membrane to the interstitial space, where it is again bound to albumin.…”

Section: Introductionmentioning

confidence: 99%

“…This could be expected since applying linear C F decrease at t=0 resembles more the final, steady-state situation than Eqs. (16)- (18), which are of the delta function type. Applying Eqs.…”

Section: Initial Conditionsmentioning

confidence: 99%

“…(13)] was replaced by a quadrature (parabolic dependence of C F on x) the differences became minor and insignificant within less than 5 s. This reflects the tendency of the solution to reach an equilibrium within a short time. Perhaps a more "natural" choice of initial conditions is the following: assume that there is a region that contains only albumin (with C A T concentration) and at a certain moment (t=0) we attach this region to the source, i.e., at x=0 this region has C A , C F , C AF values that are identical to the source equilibrium values, but the rest of the region (x>0) is still unaffected, specifically, (16) (17) (18) where the source concentrations are uniquely determined by Eqs. (4)- (6).…”

Section: Initial Conditionsmentioning

confidence: 99%

See 1 more Smart Citation

Facilitated Diffusion and Membrane Permeation of Fatty Acid in Albumin Solutions

Barta¹,

Sideman²,

Bassingthwaighte³

2000

Annals of Biomedical Engineering

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Facilitated transport is characteristic of most living systems, and usually involves a series of consecutive adjacent transfer regions, each having different transport properties. As a first step in the analysis of the multiregional problem, we consider in a single unstirred layer the facilitated diffusion of fatty acid (F) in albumin (A) solution under conditions of slow versus rapid associationdissociation, accounting for differing diffusivities of the albumin-fatty acid complex (AF). Diffusion gradients become established in an unstirred layer between a source of constant concentration of A, AF, and F in equilibrium, and a membrane permeable to F. The posited system does not reduce to a thin-or thick-layer approximation. The transient state is prolonged by slower on/off binding rates and by increasing the thickness of the unstirred layer. Solutions to transient and steady state depend the choice of boundary conditions, especially upon for thin regions. When there are two regions (each with its specific binding protein) separated by a permeable membrane, the steady-state fluxes and concentration profiles depend on the rates of association and dissociation reactions, on the diffusion coefficients, local consumption rates, and on the membrane permeability. Sensitivity analysis reveals the relative importance of these mechanisms.

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Fatty acid oxidation capacity and fatty acid-binding protein content of different cell types isolated from rat heart

Cited by 8 publications

References 19 publications

Strategies to Enhance Fat Utilisation During Exercise

Strategies to Enhance Fat Utilisation During Exercise

The capillary transport system for free fatty acids in the heart.

Facilitated Diffusion and Membrane Permeation of Fatty Acid in Albumin Solutions

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