Fatty acid control of growth of human cervical and endometrial cancer cells

Gleeson, R.; Ayub, Mahmood; Wright, Jeremy; Wood, C. B.; Habib, Nagy; Soutter, W. P.; Sullivan, M.; White, John O.

doi:10.1038/bjc.1990.113

Cited by 18 publications

(15 citation statements)

References 23 publications

(23 reference statements)

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“…Spector & Burns, 1987); this also increased cell sensitivity to adriamycin (Spector & Burns, 1987). Stearic acid, an 18-carbon fatty acid, has also been shown to inhibit the growth of human cervical cells (Gleeson et al, 1990). It is, perhaps, unlikely that a 4-carbon fatty such as n-butyrate would become directly incorporated into the cell membrane, it is, however, conceivable that it could modify the action of certain enzymes involved in membrane synthesis and/or influence cellular levels of such enzymes.…”

Section: Discussionmentioning

confidence: 99%

The effect of sodium butyrate on the growth characteristics of human cervix tumour cells

Dyson¹,

Daniel²,

Surrey³

1992

Br J Cancer

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Summary Sodium butyrate has been shown to affect cell proliferation, and, at concentrations above approximately 0.5 mM, to cause cell death in some tumour cell lines. When combined with cytotoxic drugs increase in chemosensitivity has been observed. We are presently carrying out a study of the combined effects of sodium butyrate and cytotoxic drugs on cultured cervix tumour cells. To provide a baseline for this study we Prasad, 1980;Kruh, 1982;Nordenberg et al., 1987). Possibly combined with the effect of n-butyrate on cell proliferation is its action in reversibly inducing what has been termed a 'better differentiated phenotype', of increased radiosensitivity and chemosensitivity (Spremuli & Dexter, 1984). Due to these properties n-butyrate has been the subject of clinical investigations in leukaemic patients (Novogrodsky et al., 1983;Miller et al., 1987). Its effects when combined with cytotoxic drugs (Wasserman et al., 1989) or irradiation (Arundel & Leith, 1987; Leith et al., 1986) have also been investigated.We are presently carrying out an investigation of the effect of n-butyrate, when combined with a range of cytotoxic drugs, on cervix tumour cell lines when cultured as multicell spheroids. As a necessary preliminary to this study we have carried out a systematic investigation of the effect of nbutyrate alone on the growth characteristics of cervix tumour cells cultured as multicell spheroids. The effect of n-butyrate concentrations in the range 0.005 to 3 mM has been investigated, and the culture of the cells as multicell spheroids has allowed measurements over periods of up to 24 days. The results of this study are presented in this report. Materials and methods Cell cultureThe cervix tumour cell lines employed in this study were established in primary culture from cervix biopsy tissue taken routinely during radiotherapy at Cookridge Hospital (Dyson et al., 1984a). Six cell lines were used: 754, 612, 995, 090, 329, 708. These were maintained as monolayer cultures from which multicellular aggregates were obtained as required by using the method of Sutherland and Durand (1976). Spheroid cultures were initiated with the same number of spheroids per flask to allow intercomparison of cell counts during the period of the experiments. Multicell spheroids were maintained in culture as previously described (Boothby et al., 1989). Excess spheroids were discarded at the time of media change to maintain cell numbers approximately constant.The necessary volumes of 50 mM or 500 mM sodium nbutyrate solution were added to the spheroid suspensions at the start of the experiment to adjust to the concentrations shown in the figures, with further additions at media changes to maintain these concentrations. The butyrate solution was prepared from n-butyric acid (BDH Limited, Poole, UK) in Hanks basic salt solution, adjusted to pH 7.2 with NaOH solution, then sterilised by filtration through an 0.2 gtm Acrodisc (Gelman Sciences Limited, Northampton, UK).Spheroid diameter This was measured by means of a laser diffracti...

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Section: Discussionmentioning

confidence: 99%

The effect of sodium butyrate on the growth characteristics of human cervix tumour cells

Dyson¹,

Daniel²,

Surrey³

1992

Br J Cancer

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“…This compound was also detected in the bioactive (ethanolic acid) fraction of S. trilobatum inhibiting Elrich's Ascites Carcinoma growth [21]. Stearic acid, a C18 long chain fatty acid was also present in both the fractions reported to have inhibitory activity against cervical cancer line HOG-1 [22]. Palmitic acid present in SNC, showed selective cytotoxicity to MOLT-4 (Human leukemic) cell line, probably by inhibiting DNA topoisomerase-I [23].…”

Section: Discussionmentioning

confidence: 99%

Induction of Apoptosis by Fatty Acid Rich Fraction of Solanum Nigrum on Cervical Cancer Cell Lines

Paul

Kundu

2017

Int J Pharm Pharm Sci

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Objective: Mechanism of cell death inducing pathway of chloroform fraction of methanolic extract of Solanum nigrum were studied in a panel of cervical cancer cell line.Methods: Cells were treated with IC50 doses (previously determined by MTT assay), and various experiments were performed. For cellular and nuclear morphology, cells were stained with Hoechst33258 and observed under phase and fluorescence microscopy. Cell cycle shift and apoptotic assay (AnnexinV-FITC/PI) were studied by flow cytometry. Expressions of relevant genes were studied at the transcriptional and translational levels. ROS induction and loss of MMP and presence of γH2AX were studied by fluorescent microscopy. Constituents of the fraction were separated by column chromatography and bioassayed by MTT assay. Bioactive sub-fractions were identified by GC-MS.Results: Typical apoptotic morphological features were found in the treated cells. Most cells were found in late apoptotic stages. In HeLa and C33A cell lines, cells were blocked at G1/S, in SiHa, subG0 population increased. Expression of antiapoptotic Bcl-2 decreased and that of proapoptotic BAX, p53, p21 increased. Expression of HPV16 and human ET1 also decreased. ROS mediated DNA damage was detected and induced apoptosis. Different fatty acids along with other compounds were detected in the chloroform fraction, all of which are reported to have anticancer properties. Conclusion:From the study it can be concluded that fatty acid rich chloroform fraction of S. nigrum is capable of inducing apoptosis in cervical cancer cells, through ROS mediated DNA damage.

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“…The present study indicates that malignant transformation, and to a slightly lesser extent cell im mortalisation which invariably preceded transforma tion, can directly be suppressed by SA derivatives. The effect of these compounds on transformation in vitro is more complete than their control of tumour cell growth (growth rates are reduced rather than prevented [5][6][7]), so it is suggested that their effect on in vivo carcinogen esis is more likely to be at the level of premalignant changes than on incipient tumours.…”

Section: Discussionmentioning

confidence: 99%

“…It has not been possible to decide whether inhibition of carcinogenesis in vivo is due to reduced growth potential of small (undetected) primary tumours, analogous to the inhibitory effect of SA or ISA on tumour cells in culture [5][6][7], or to suppression of malig nant transformation in target tissues affected by the car cinogen. The present study indicates that malignant transformation, and to a slightly lesser extent cell im mortalisation which invariably preceded transforma tion, can directly be suppressed by SA derivatives.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro, both saturated [4][5][6][7] and polyunsaturated [8][9][10] fats or fatty acids can have markedly inhibitory effects on the growth rate and clonogenicity of established human and rodent tumour cells. A particularly interesting extension of these phenomena was the finding that stearic acid and its derivative 9,10-iodostearic acid (ISA) could suppress primary mammary carcinogenesis in vivo induced in rats by N-nitrosomethylurea [6], However, it was not possible to conclude from the model used whether the fatty acid was suppressing the growth of incipient tu mour cells following transformation by the carcinogen or whether it could influence preneoplastic events and thereby prevent transformation from occurring.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of Chemically Induced Neoplastic Transformation in vitro by Saturated Fatty Acids

Embleton

Noy²

1991

Pathobiology

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Syrian hamster embryo cells were treated for 2–4 days with 3-methylcholanthrene (MCA), and 28 days after application of the carcinogen they were exposed continuously to 12-O-tetradecanoylphorbol-13-acetate (TPA). Untreated cells, or cells treated with MCA or TPA only, usually became senescent around 6–8 weeks after plating and died, but those treated with both MCA and TPA became immortalised and underwent transformation to a phenotype capable of growth in soft agar. Transformation was inhibited by derivatives of stearic acid administered to the cells at various stages of the overall process. Some derivatives were active at lower concentrations than others, but none were selective for specific phases such as initiation or promotion, and it did not appear to matter whether the fatty acids were present throughout the assay or for only part of it. Their action seems to be associated with control of growth or differentiation in a relatively non-specific manner.

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Fatty acid control of growth of human cervical and endometrial cancer cells

Cited by 18 publications

References 23 publications

The effect of sodium butyrate on the growth characteristics of human cervix tumour cells

The effect of sodium butyrate on the growth characteristics of human cervix tumour cells

Induction of Apoptosis by Fatty Acid Rich Fraction of Solanum Nigrum on Cervical Cancer Cell Lines

Inhibition of Chemically Induced Neoplastic Transformation in vitro by Saturated Fatty Acids

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