Fatty acid content in muscles of amago salmon homozygous or heterozygous for a growth hormone transgene

Sugiyama, Manabu; Takenaga, Fumio; Okamoto, Hiroyuki; Masaoka, Tetsuji; Araki, Kazuo; Nagoya, Hiroyuki; Mori, Tsukasa

doi:10.1016/j.aquaculture.2014.10.020

Cited by 2 publications

(1 citation statement)

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“…These transgenic fish exhibited morphological changes in the liver tissues 16 , significantly elevated concentrations of plasma GH1 and insulin-like growth factor 1, and decreased total cholesterol and triglyceride levels 17 compared to wild-type fish. Moreover, homozygous (ectopic GH, Tg derived from both female and male, ♀/♂, Tg/Tg) and hemizygous GH-transgenic (ectopic GH derived from the female fish, Tg/+) fish exhibit decreased serum glucose levels, increased GRP78 (glucose starvation gene) expression, and increased ketone body (3-hydroxybutyric acid) levels 18 , as well as decreased levels of saturated fatty acids (14:0) and monounsaturated fatty acids (16:1n-7, 18:1n-9) in the liver and muscle tissues 19 .…”

Section: Introductionmentioning

confidence: 99%

A novel ND1 mitochondrial DNA mutation is maternally inherited in growth hormone transgenesis in amago salmon (Oncorhynchus masou ishikawae)

Satō

Goto‐Inoue

Kimishima

et al. 2022

Sci Rep

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Growth hormone (GH) transgenesis can be used to manipulate the growth performance of fish and mammals. In this study, homozygous and hemizygous GH-transgenic amago salmon (Oncorhynchus masou ishikawae) derived from a single female exhibited hypoglycemia. Proteomic and signal network analyses using iTRAQ indicated a decreased NAD+/NADH ratio in transgenic fish, indicative of reduced mitochondrial ND1 function and ROS levels. Mitochondrial DNA sequencing revealed that approximately 28% of the deletion mutations in the GH homozygous- and hemizygous-female-derived mitochondrial DNA occurred in ND1. These fish also displayed decreased ROS levels. Our results indicate that GH transgenesis in amago salmon may induce specific deletion mutations that are maternally inherited over generations and alter energy production.

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Section: Introductionmentioning

confidence: 99%