A novel ND1 mitochondrial DNA mutation is maternally inherited in growth hormone transgenesis in amago salmon (Oncorhynchus masou ishikawae)

Abstract

Growth hormone (GH) transgenesis can be used to manipulate the growth performance of fish and mammals. In this study, homozygous and hemizygous GH-transgenic amago salmon (Oncorhynchus masou ishikawae) derived from a single female exhibited hypoglycemia. Proteomic and signal network analyses using iTRAQ indicated a decreased NAD+/NADH ratio in transgenic fish, indicative of reduced mitochondrial ND1 function and ROS levels. Mitochondrial DNA sequencing revealed that approximately 28% of the deletion mutations in the GH homozygous- and hemizygous-female-derived mitochondrial DNA occurred in ND1. These fish also displayed decreased ROS levels. Our results indicate that GH transgenesis in amago salmon may induce specific deletion mutations that are maternally inherited over generations and alter energy production.

Roles of Mitochondrial DNA Damage in Kidney Diseases: A New Biomarker

“…Homogeneous mutations refer to the mutation of all mtDNA in mitochondria, whereas heterogeneous mutations refer to the coexistence of mutant and wild-type mtDNA. mtDNA mutations have the cumulative effect of defective mitochondrial function, causing impairment of a cellular energy supply [ 25 ]. The biological consequences of mutations depend on the proportion of mutant mtDNA and the type of mutation carried by the cells.…”