Fatty Acid Composition of Hepatopancreas and Gonads in Both Sexes of Orange Mud Crab, Scylla olivacea Cultured at Various Water Flow Velocities

Abstract

Nutritional quality of the hepatopancreas and gonads of orange portunid mud crab, Scylla olivacea was evaluated for each gender under four treatment of different water velocities (0, 20, 40 and 60 cm s −1 ), in terms of nutrient reserve and nutrient for reproduction. About 56 crabs were used in this study in which fatty acids composition was analysed using gas chromatography mass-spectrometry (GC-MS). For hepatopancreas analysis, monounsaturated fatty acids (MUFAs) were present in the highest fatty acids concentration, followed by polyunsaturated fatty acids (PUFAs) and, saturated fatty acids (SFAs). However, long-chain polyunsaturated fatty acids (LC-PUFAs) were displayed in low concentration in the hepatopancreas. Total fatty acid (TFAs) composition was significantly higher at moderate velocity of 20 cm s −1 compared to other water velocity treatments. For gonad analysis, 20 cm s −1 showed the highest TFA concentration of 93.34 mg g −1 while, the lowest concentration of 3.90 mg g −1 occurred at 0 cm s −1 . There were significant differences in male and female crab’s fatty acids contents of gonads at all flow velocities challenged ( p < 0.05). PUFAs and MUFAs were dominant while, SFAs were observed at low concentration. This study revealed that, concentration of PUFAs increased as gonad maturation increased. The decreasing concentration of hepatopancreas fatty acids over the culture period indicated that nutrient was shifted from the hepatopancreas, to be used as energy reserved to gonads for further growth of eggs and offspring. The linkages between water flow strength, hepatopancreas, and gonad fatty acids concentrations, is fundamental knowledge useful in establishing efficient habitat velocities selection which will improve aquaculture production of mud crabs with high quality broodstock.

Enhancement of protein, lipid and fatty acids in copepod, Oithona rigida for the improvement of growth, development and survival of early‐stage mud crab larvae ( Scylla olivacea )

“…The fatty acid of newly hatched larvae S. olivacea was shown in Tables 14 and 15 to be influenced by the food eaten by larvae through the metamorphosis process, same with another marine crustacean (Taufik et al, 2016). Lim et al (2021), Taufik et al (2020), Waiho et al (2018) have shown that fatty acid compositions have been highlighted due to the substantial effect of essential polyunsaturated fatty acids (PUFAs) such as DHA and EPA on consumer's survival and egg production. After the nutrients were ingested, PUFA was converted into storage lipids or into structural lipids form such as cell membranes (Taufik et al, 2020).…”

“…Lim et al (2021), Taufik et al (2020), Waiho et al (2018) have shown that fatty acid compositions have been highlighted due to the substantial effect of essential polyunsaturated fatty acids (PUFAs) such as DHA and EPA on consumer's survival and egg production. After the nutrients were ingested, PUFA was converted into storage lipids or into structural lipids form such as cell membranes (Taufik et al, 2020). According to (Wang et al, 2021), EPA and DHA composition are better than C18 PUFAs to sustain the growth of crustaceans such as P. japonicus .…”

“…The fatty acid of newly hatched larvae S. olivacea was shown in Tables 14 and 15 to be influenced by the food eaten by larvae through the metamorphosis process, same with another marine crustacean (Taufik et al, 2016). branes (Taufik et al, 2020). According to (Wang et al, 2021), EPA and DHA composition are better than C18 PUFAs to sustain the growth of crustaceans such as P. japonicus.…”

Insight of vitellogenesis patterns: A comparative analysis of the differences between the primary and secondary vitellogenesis period in the ovary, hepatopancreas, and muscle of mud crab, scylla paramamosain

“…Amino acids serve as an essential nutrient source for ovarian maturation, embryonic development, spawning, and hatching ( Peñaflorida, 2004 ). Previous studies have revealed the biochemical compositions of the edible parts of S. serrata ( Peñaflorida, 2004 ; Romano et al, 2014 ), S. olivacea ( Taufik et al, 2020 ), and S. paramamosain ( Jiang et al, 2014 ; Tantikitti et al, 2015 ). Wu et al (2020) clarified the dynamics of the biochemical composition, including lipids and amino acids, in the ovary, hepatopancreas, and muscle of S. paramamosain during the ovarian maturation period.…”