“…This plant is a legume belonging to the Fabaceae family (Suryanti et al, 2016;Verma, 2016;Abdelhay & Abdallah, 2016). The common names of L. leucocephala include petai cina or lamtoro (Indonesia), white lead tree (England), and subabool (India; Soetjipto et al, 2019;Verma, 2016). The seeds and leaves of this plant contain mimosine/leucaenin/leucenol/leucaenol (Nguyen & Tawata, 2016), terpene, flavonoids, cumarin, sterol, ficaprenol-11, scualene, lupeol, β sitostenone, trans-cumaric acid, cis-cumaric acid, pheophytin-a, pheophorbide a-methyl ester, and aristophyll-C (Chen & Wang, 2010).…”
“…This plant is a legume belonging to the Fabaceae family (Suryanti et al, 2016;Verma, 2016;Abdelhay & Abdallah, 2016). The common names of L. leucocephala include petai cina or lamtoro (Indonesia), white lead tree (England), and subabool (India; Soetjipto et al, 2019;Verma, 2016). The seeds and leaves of this plant contain mimosine/leucaenin/leucenol/leucaenol (Nguyen & Tawata, 2016), terpene, flavonoids, cumarin, sterol, ficaprenol-11, scualene, lupeol, β sitostenone, trans-cumaric acid, cis-cumaric acid, pheophytin-a, pheophorbide a-methyl ester, and aristophyll-C (Chen & Wang, 2010).…”
“…Column chromatography silica gel has been applied as a technique for the fractionation of lipids from various sources. [27][28][29] In their study, Sajilata et al [30] successfully separated monoglycerides and diglycerides from Spirulina platensis using silica gel column chromatography. Recently, small commercial prepacked columns with a variety of solid stationary phases called solid-phase extraction (SPE) were introduced for the fractionation of lipids.…”
Section: Practical Applicationsmentioning
confidence: 99%
“…Column chromatography silica gel has been applied as a technique for the fractionation of lipids from various sources. [ 27–29 ] In their study, Sajilata et al. [ 30 ] successfully separated monoglycerides and diglycerides from Spirulina platensis using silica gel column chromatography.…”
With the global demand for lipid compounds on the rise, concerns are growing about the environmental and economic impact of traditional lipid sources. This concern is exacerbated by the ever-increasing demand for plant-based lipids, which is contributing to unsustainable production practices and competition for land and food.Considering this challenge, this work aimed at exploring the potential of black soldier fly larvae (BSFL) as a low-cost and ecofriendly source of lipid classes. A fractionation scheme consisting of a mixture of polar and nonpolar solvents at different ratios was employed to isolate the lipid classes from BSFL oil using silica gel column chromatography, which is a conventional method of chromatography. The fraction's separation efficiency was validated using thin-layer chromatography and characterized with Fourier-transformed infrared spectroscopy and gas chromatography flame ionization (GC-FID). Triglycerides (30%) were found to be the most abundant component while cholesterols (0.6%) were the least abundant lipid fractions in the lipid mixture. GC-FID in the various lipid fractions analyzed, lauric acid exhibited the highest percentage among the triglycerides (16.64%), diglycerides (19.10%), monoglycerides (22.70%), and free fatty acids (27.65%) fractions. The fractionation scheme proposed achieves high efficiency in separating and recovering different lipid classes extracted from the BSFL.
“…de Wit is a tropical leguminous tree in the family of Leguminosae found in Indonesia and the other tropical areas (Syamsudin et al 2010;Verma 2016). The common name of L. leucocephala is petai cina or lamtoro (Indonesia) and white lead tree (English) (Soetjipto et al 2019). Parkia speciosa Hassk.…”
Abstract. Wardatun S, Harahap Y, Mun’im A. 2020. Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations. Biodiversitas 21: 1060-1064. Leucaena leucocephala (Lam.) de Wit (petai Cina), Parkia speciosa Hassk. (petai), and Archidendron jiringa (Jack) I.C. Nielsen (jengkol) seeds extracts contain sulfhydryl compounds and have various therapeutic properties. The main objective of this research was to compare the effect of various ethanol concentration on the yield of extract and levels of sulfhydryl compounds from leguminous seeds. Dried seeds were macerated at room temperature (25°C) with a ratio of solids and solvents was 1:10. The solvent concentrations used were 30%, 50%, 70% and 96% ethanol. The yield of extract was expressed as the ratio of the weight of extract to the weight of dried seeds, the level of sulfhydryl compound was expressed as reduced L-glutathione (GSH) equivalent. The level of sulfhydryl compounds was determined by Ellman reagent and further analyzed using a spectrophotometer at 411 nm wavelength. Stink bean produced the highest yield of extract, while the highest level of sulfhydryl compounds was obtained from petai cina seed extract. The concentration of ethanol gave a significant difference to the yield of extract and the level of sulfhydryl compounds. The results showed that ethanol concentration affected the efficiency of the extraction of total sulfhydryl compounds and the yield of the extract on leguminous seeds.
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