Fatty acid-binding proteins – insights from genetic manipulations

Haunerland, Norbert H.; Spener, Friedrich

doi:10.1016/j.plipres.2004.05.001

Cited by 390 publications

(339 citation statements)

References 106 publications

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“…31 It was later found to be expressed in many other tissues, including mammary gland. 32,33 The physiological function of this protein remains elusive, and evidence supporting a role for FABP5 in human cancers is limited. Up-regulation of FABP5 RNA and protein has been observed in prostate and breast cancer cell lines relative to benign cell lines.…”

Section: Discussionmentioning

confidence: 99%

Association of FABP5 Expression With Poor Survival in Triple-Negative Breast Cancer

Liu

Graham

Glubrecht

et al. 2011

The American Journal of Pathology

140

124

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Recent studies using animal models suggest that expression of FABP5 drives the stimulation of cell growth observed in estrogen receptor (ER)-negative breast cancer cells on exposure to retinoic acid (RA). The purpose of this study was to investigate the clinicopathological significance of FABP5 in breast cancer and to evaluate FABP5 as a prognostic marker and a possible novel therapeutic target in breast cancer. Gene expression microarray analysis revealed a significant correlation between elevated FABP5 RNA levels and ER/ progesterone receptor (PR)-negative status, high tumor grade, and poor prognosis. Tissue microarray analysis demonstrated similar correlations with cytoplasmic FABP5 protein. Based on multivariate proportional regression analysis, cytoplasmic FABP5 is a significant and independent prognostic marker of overall survival and recurrence-free survival in breast cancer. The effects of FABP5 on tumor growth appear to be mediated primarily through cytoplasmic FABP, because no correlation was found between nuclear FABP5 and ER/PRnegative status, recurrence, and survival. FABP5 knockdown in breast cancer cell lines demonstrates a correlation between FABP5 levels and growth response to RA. We propose a model whereby growth-promoting FABP5 competes with growth-inhibiting CRABP2 for RA, with retention of RA in the cytoplasm by FABP5 preventing the inhibition of tumor growth. (Am J Pathol

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Section: Discussionmentioning

confidence: 99%

Association of FABP5 Expression With Poor Survival in Triple-Negative Breast Cancer

Liu

Graham

Glubrecht

et al. 2011

The American Journal of Pathology

140

124

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“…8,9 Genetic ablation of most FABPs, including FABP1 (L-FABP, expressed mainly in the liver), FABP3 (H-FABP, expressed mainly in the heart) or FABP4 (A-FABP, expressed mainly in adipose tissue) reveals an absence of a significant morphological phenotype, suggesting a functional redundancy among FABP family members. 9 We recently found that hypoxia enhances the accumulation of fat in primary human trophoblasts (PHTs) and surmised that this process is coupled to increased expression of proteins associated with intracellular fat transport, which might alter fat availability to the developing fetus. We therefore tested the hypothesis that hypoxia upregulates the expression of FABPs in PHTs.…”

Section: Introductionmentioning

confidence: 99%

Hypoxia regulates the expression of fatty acid–binding proteins in primary term human trophoblasts

Biron‐Shental

Schaiff

Ratajczak

et al. 2007

American Journal of Obstetrics and Gynecology

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Objective-Fatty acids (FA) are essential for fetal development. Cellular FA uptake is modulated by fatty acid binding proteins (FABPs). We hypothesized that hypoxia regulates the expression of FABPs in human trophoblasts.Study design-Primary term human trophoblasts were cultured for 72 h in either standard (O 2 = 20%) or hypoxic (O 2 < 1%) conditions. FABP expression was interrogated using PCR and western immunoblotting. Trophoblast lipid droplets were examined using BODIPY 493/503 staining.Results-We detected the expression of FABP1, 3, 4, 5 and pm, but not FABP2 or FABP6-9 subtypes in trophoblasts. Exposure to hypoxia markedly increased lipid droplet accumulation in trophoblasts. Consistent with this observation, hypoxia enhanced the expression of FABP1, 3 and 4. Lastly, agonists of peroxisome proliferator activated receptor-γ (PPARγ) enhanced the expression of FABP1 and 4 in trophoblasts.Conclusions-Hypoxia enhances the expression of FABP1, 3 and 4 in term human trophoblasts, suggesting that FABPs support fat accumulation in the hypoxic placenta.

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“…One candidate is brain lipid-binding protein (Blbp, Fabp7), a gene that is dynamically regulated in radial glia by migrating neurons (Feng et al 1994;Kurtz et al 1994;Feng and Heintz 1995). BLBP is a member of the large family of hydrophobic ligand-binding proteins (FABPs), molecules that have been shown to modulate transcription through their interactions with nuclear receptors and to play roles in metabolism (Haunerland and Spener 2004). Immunoelectron microscopy has demonstrated that BLBP is present in both the cytoplasm and nucleus in vivo, suggesting that similar to other FABPs, BLBP may be involved in the trafficking of a ligand to a nuclear receptor (Feng et al 1994).…”

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confidence: 99%

Brain lipid-binding protein is a direct target of Notch signaling in radial glial cells

Anthony¹,

Mason²,

Gridley³

et al. 2005

Genes Dev.

207

172

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Radial glia function during CNS development both as neural progenitors and as a scaffolding supporting neuronal migration. To elucidate pathways involved in these functions, we mapped in vivo the promoter for Blbp, a radial glial gene. We show here that a binding site for the Notch effector CBF1 is essential for all Blbp transcription in radial glia, and that BLBP expression is significantly reduced in the forebrains of mice lacking the Notch1 and Notch3 receptors. These results identify Blbp as the first predominantly CNS-specific Notch target gene and suggest that it mediates some aspects of Notch signaling in radial glia. Received February 2, 2005; revised version accepted March 25, 2005. During the development of the mammalian brain, billions of neurons have to be generated, sent to their appropriate locations, and then interconnected in a precise manner. This task is particularly complex in the cerebral cortex, where an inside-out mode of development results in newly generated neurons having to migrate over larger distances through increasingly crowded terrain as neurogenesis proceeds (Angevine and Sidman 1961). Numerous studies have documented the critical role played by the radial glial scaffold in facilitating this migration, and estimates are that as much as 90% of neuronal migration in the cortex may take place upon radial glial cells (Sidman and Rakic 1973;Hatten 1999). In addition to their structural role, radial glial cells also function as neuronal progenitors (Malatesta et al. 2000;Miyata et al. 2001;Noctor et al. 2001;Tamamaki et al. 2001) and have been shown to be the source of most neurons throughout the CNS (Anthony et al. 2004).The dual role played by radial glia as both migratory scaffolding and neuronal progenitors has suggested that there may be intimate links between the signaling pathways that control radial glial cell development, neuronal generation, and neuronal migration. The fact that migrating neurons regulate morphological and proliferative characteristics of glia (Hatten 1985) has led to considerable focus on neuronally derived signals, and several cell surface and diffusible molecules have been implicated in neuronal-radial glial signaling, including Astrotactin (Zheng et al. 1996), Neuregulin/ErbB2 (Anton et al. 1997;Rio et al. 1997;Patten et al. 2003;Schmid et al. 2003), and Notch (Gaiano et al. 2000;Gaiano and Fishell 2002;Patten et al. 2003;Schmid et al. 2003). In contrast, the genes expressed within radial glia that mediate the radial glial response to neuronal signaling are not well characterized. One candidate is brain lipid-binding protein (Blbp, Fabp7), a gene that is dynamically regulated in radial glia by migrating neurons (Feng et al. 1994;Kurtz et al. 1994;Feng and Heintz 1995). BLBP is a member of the large family of hydrophobic ligand-binding proteins (FABPs), molecules that have been shown to modulate transcription through their interactions with nuclear receptors and to play roles in metabolism (Haunerland and Spener 2004). Immunoelectron microscopy has demo...

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Fatty acid-binding proteins – insights from genetic manipulations

Cited by 390 publications

References 106 publications

Association of FABP5 Expression With Poor Survival in Triple-Negative Breast Cancer

Association of FABP5 Expression With Poor Survival in Triple-Negative Breast Cancer

Hypoxia regulates the expression of fatty acid–binding proteins in primary term human trophoblasts

Brain lipid-binding protein is a direct target of Notch signaling in radial glial cells

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