Fatty Acid and Polar Lipid Composition in the Classification of Kurthia

Abstract

Strains of Kurthia zopfii were degraded by acid methanolysis and the non‐hydroxylated fatty acid esters so released were examined by gas liquid chromatography. The major fatty acid types were straight‐chain, anteiso‐ and iso‐methyl branched‐chain acids. Monounsaturated fatty acids were not detected. The major fatty acid in five of the six strains examined consisted of 12‐methyltetra‐decanoic (anteiso‐C15) acid. The other strain possessed major amounts of both 13‐methyltetradecanoic (iso‐C15) and anteiso‐C15 acids. Polar lipids of all the strains were examined by two‐dimensional thin‐layer chromatography. All possessed a very simple polar lipid composition consisting of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.

Chemotaxonomic Study of an Alkalophilic Bacterium, Exiguobacterium aurantiacum gen. nov., sp. nov.

“…Although PE is common amongst Bacillus species, the latter also possess glycolipids (Shaw, 1970;Lechevalier, 1977). It is worth noting that Kurthia zopfii also possesses the simple polar lipid pattern DPG, PG and PE (Goodfellow et a/., 1980).…”

1,8‐Bis(dimethylamino)naphthalene: a novel superbasic matrix for matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric analysis of fatty acids

“…ROS), 2,3 and classifying species taxonomically, [4][5][6][7] has led to dramatic advances in the analysis of complex lipids. However, such analysis still depends on lowthroughput gas chromatography (GC) with electron ionization (EI-MS) [8][9][10] or chemical ionization mass spectrometry (CI-MS).…”

The Genomic Heterogeneity among Mycobacterium terrae Complex Displayed by Sequencing of 16S rRNA and hsp65 Genes

“…Other methods such as thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) are more powerful, but are cumbersome, expensive and limited by the need for standardized growth conditions. Currently, these methods are used in very few clinical laboratories (1,5,8).In addition to these phenotypic methods, genotypic and phylogenetic methods were introduced for polyphasic taxonomy and complete identification of microor- Abstract: The species identification within Mycobacterium terrae complex has been known to be very difficult. In this study, the genomic diversity of M. terrae complex with eighteen clinical isolates, which were initially identified as M. terrae complex by phenotypic method, was investigated, including that of three type strains (M. terrae, M. nonchromogenicum, and M. triviale).…”

“…Other methods such as thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) are more powerful, but are cumbersome, expensive and limited by the need for standardized growth conditions. Currently, these methods are used in very few clinical laboratories (1,5,8).…”