Fatty Acid and Lipid Class Composition of the Microalga &lt;i&gt;Phaeodactylum tricorn&lt;/i&gt;&lt;i&gt;utum&lt;/i&gt;

Yang, Yuhong; Du, Lei; Hosokawa, Masashi; Miyashita, Kazuo; Kokubun, Yume; Arai, Hisayoshi; Taroda, Hiroyuki

doi:10.5650/jos.ess16205

Cited by 51 publications

(49 citation statements)

References 27 publications

(42 reference statements)

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“…The main lipid components were mid-chain FAs, 14:0, 16:0, 16:1n-7, and 20:5n-3, which together represented 76.46% of total fatty acids ( Table 4). Our results are similar to those reported by [42]. Palmitoleic acid (16:1n-7), which constituted 28.20% of the total FAs, was predominant in P. tricornutum control (W) but was reduced to 24.29% in RS condition (Appendix A; Table A1).…”

Section: Fatty Acid Methyl Esters Analysis (Fames)supporting

confidence: 91%

“…P. tricornutum produces lipids that are potentially significant for several industrial applications. It has been reported that the crude lipids extracted from P. tricornutum are around 321.89 mg/g dry weight [42] or 34% of dry weight [43] and are comparable to the lipid extracted in W in our study, which is 0.4 g/g dry weight in control white light. Furthermore, the lipid dry weight in RS was significantly increased to 2.3-fold and 1.8-fold as compared to W and R, respectively ( Figure 3B).…”

Section: Analysis Of Total Biomass and Lipid Content Under Red Light supporting

confidence: 78%

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Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

et al. 2020

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Marine water diatom Phaeodactylum tricornutum is a photosynthetic organism that is known to respond to the changing light environment and adapt to different temperatures to prevent photoinhibition and maintain its metabolic functions. The objective of the present study was to test whether light shift variations in different growth phases impact the growth and lipid metabolism of P. tricornutum. Thus, we investigated R exposure in different growth phases to find the most effective light shift condition. The results showed that substituting white light (W) by red light (R) under autotrophic conditions, a condition called red shift (RS), increased biomass and lipid content compared to levels found under continuous W or R exposure alone. We observed an increase by 2-fold biomass and 2.3-fold lipid content in RS as compared to W. No significant change was observed in the morphology of lipid droplets, but the fatty acid (FA) composition was altered. Specifically, polyunsaturated FAs were increased, whereas monounsaturated FAs decreased in P. tricornutum grown in RS compared to W control. Therefore, we propose that a light shift during the beginning of the stationary phase is a low-cost cultivation strategy to boost the total biomass and lipids in P. tricornutum.Appl. Sci. 2020, 10, 2531 2 of 18 for the high production of desired bioactive molecules. Therefore, light should be provided with specific spectral components, intensity, and duration. Multiple ecophysiological studies have been published reporting on the distinct behaviors of different diatoms and algae in response to variations in light [4,5]. It has been shown that light quality has an impact on chloroplast migration and on the light acclimation reactions of photosynthesis [6,7]. Red light has been linked to stimulating growth, ethylene production [8], lipid accumulation [7], and increasing thylakoid stacking [6]. have suggested the involvement of phytochromes in increasing the intracellular nitrate content on exposure to red light for short periods altering the overall cellular nitrate content. Recent discoveries in genomics have revealed exciting information on phytochromes, blue-light sensing cryptochromes, and aureochromes [10]. In a study by Jungandreas et al. [11], it has been reported that P. tricornutum cells acclimated to both red and blue light have comparable metabolite profiles, but drastic changes in metabolites and carbon partitioning was observed under red to blue light shift. In addition, various interdisciplinary studies have shown that light perceived by the red-light receptor (phytochromes) can regulate nutrient metabolism, cellular events, and signaling cascades [12]. These discoveries are still in progress and much remains unknown, but these studies can be explored for application-based diatom experiments such as developing low-cost cultivation technology or efficient bioreactors.In both culture medium and natural water bodies, light (intensity, distribution, photoperiod) may vary and will not behave similar to the dissolved salts or nu...

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Section: Fatty Acid Methyl Esters Analysis (Fames)supporting

confidence: 91%

Section: Analysis Of Total Biomass and Lipid Content Under Red Light supporting

confidence: 78%

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

et al. 2020

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“…In general, neutral lipids are the main lipid fraction found in diatoms [7,[21][22][23]. The production of TAG by H. coffeaeformis increased with the number of days in culture in the photobioreactor, and its value (in mg·L −1 ) was comparable to that reported for another diatom from Bahía Blanca Estuary grown under similar conditions [24].…”

Section: Discussionsupporting

confidence: 69%

“…In general, neutral lipids are the main lipid fraction found in diatoms [7,[21][22][23]. The production…”

Section: Discussionmentioning

confidence: 99%

Assessment of Halamphora coffeaeformis Growth and Biochemical Composition for Aquaculture Purposes

Bilbao

Martín

Popovich

et al. 2020

JMSE

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The vast majority of the world's fishery by-products are utilized in the fish farming industry. However, due to the high cost and unsustainability of these by-products, alternative sources must be found. Marine diatoms produce important amounts of fatty acids, sterols, proteins and carbohydrates. In this work, we assessed the nutritional value of Halamphora coffeaeformis at the exponential growth phase, to determine its suitability for aquafeed. The strain was grown in a photobioreactor at 20 • C for 6 days. The production of proteins, lipids and carbohydrates was determined, and essential fatty acid, sterol and amino acid composition was assessed. The highest values of triacylglycerides (TAG), free and esterified sterols, proteins and carbohydrates were found after 6 days of growth in the photobioreactor. Fatty acid analysis by gas chromatography showed the presence of eicosapentaenoic (EPA) and arachidonic (ARA) in amounts similar to those for fish oils. In terms of sterols, a predominance of stigmasterol was observed, followed by cholesterol. The amino acid composition revealed 50% of them to be among those essential for fish and other aquatic animals. Finally, a suitable amount of carbohydrates was found in H. coffeaeformis cultures. Together, these findings support the use of H. coffeaeformis as an alternative and sustainable source for aquafeed to partially replace the use of fishery by-products.

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“…Total lipids of spinach and Akamoku were subjected to lipid class analysis by preparative thin layer chromatography (TLC) [14]. The lipid fraction was dis- …”

Section: Lipid Class Analysismentioning

confidence: 99%

Comparison of Oxidative Stability of Monogalactosyl Diacylglycerol, Digalactosyl Diacylglycerol, and Triacylglycerol Containing Polyunsaturated Fatty Acids

Hazahari¹,

Hosokawa²,

Miyashita³

2018

FNS

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Oxidative stability of three different lipid classes, namely, monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) from spinach and edible brown seaweed (Akamoku) and triacylglycerol (TAG) of linseed oil was compared. Analysis of oxygen consumption and polyunsaturated fatty acid (PUFA) composition demonstrated that spinach DGDG had the highest oxidative stability, followed by Akamoku DGDG, Akamoku MGDG, spinach MGDG, and linseed TAG. These results disagree with the order of oxidative stability expected from the average number of bis-allylic positions of each lipid. Additionally, DGDG constituents of both spinach and Akamoku showed higher oxidative stability than their MGDG constituents. The unusual oxidative stability of MGDG and DGDG could be conferred by the protection of bis-allylic positions of the PUFA against oxidative attack by the galactosyl moiety of the GL.

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Fatty Acid and Lipid Class Composition of the Microalga <i>Phaeodactylum tricorn</i><i>utum</i>

Cited by 51 publications

References 27 publications

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum

Assessment of Halamphora coffeaeformis Growth and Biochemical Composition for Aquaculture Purposes

Comparison of Oxidative Stability of Monogalactosyl Diacylglycerol, Digalactosyl Diacylglycerol, and Triacylglycerol Containing Polyunsaturated Fatty Acids

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