Fatty acid and elemental composition of the marine diatom Chaetoceros gracilis Schütt. Effects of silicate deprivation, temperature and light intensity

Mortensen, Stein; Børsheim, Knut Yngve; Rainuzzo, JoséRodriguez; Knutsen, Gjert

doi:10.1016/0022-0981(88)90183-9

Cited by 115 publications

(46 citation statements)

References 26 publications

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“…Adaptative acclimation to species selection, light limitation, and growth phases appeared to proceed through transient changes in the PUFA of polar lipids which are known to regulate membrane fluidity. The known association between relative concentration of PUFA and nutrlent regime (Pohl & Zurbeide 1979, Shifrin & Chisholm 1981, Enright et al 1986, Mortensen et al 1988) suggests some functjonal relationship between membrane fluidity and nutrient availability which in most culture systems is obscured during the stationary growth phase. In the present experiment, the maintenance throughout the different growth cycles of exponential growth strongly suggests that in culture or culture-like system high levels of PUFA could be related to permeability requirements to cope with the high nutrient situation.…”

Section: Discussionmentioning

confidence: 99%

“…Adaptation of membrane fatty acid constituents to growth-limiting light intensity has been little studied in marine phytoplankton species. Recently, Mortensen et al (1988) reported little effect on the total fatty acid composition of the diatom Chaetoceros gracilis. They described increased levels of highly unsaturated acids (w3 or n-3) with increasing light intensity but with hardly any variation of the unsaturated to saturated acids ratio and polyunsaturated to monoenoic plus saturated acids ratio.…”

Section: Discussionmentioning

confidence: 99%

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Effect of variable nutrient supply on fatty acid composition of phytoplankton grown in an enclosed experimental ecosystem

Mayzaud¹,

Claustre²,

Augier³

1990

Mar. Ecol. Prog. Ser.

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Outdoor experimental tanks were used to simulate phytoplankton blooms in natural seawater from the bay of Villefranche-sur-Mer (France), during March-April 1984. External temperature and light intensity were respectively monitored every 4 h and daily. Nutrient conditions were followed daily and maintained by a cycle of dilutions with fresh enriched seawater. Six different cycles of phytoplankton production were followed. They differed by the dilution rates used. Nutrient uptake. phytoplankton composition, biochemical composition and detailed lipid composition of the phytoplankton were followed throughout each growth cycle. Results showed a clear difference between the first cycle, which lasted 7 d , and subsequent ones which lasted only from 27 to 51 h In terms of species composition, the initial body of water con~prised a complex assemblage of species (flagellates, dinoflagellates, diatoms, microzooplankton) which were gradually replaced by a community of diatoms dominated by Skeletonema costatum, Nitzschia sp. and Chaetoceros sp. Total fatty acid changes reflected the progressive changes in species composition (inverse variation of C16 and C18 polyunsaturated fatty acids) and to a minor extent the nutrient regime. In terms of fatty acid composition of the different lipid classes the transition from a flagellate to a diatom population appears to b e the key factor influencing all categories in a similar way. Nutrient regime affected essentially the neutral lipid composition while changes in physiological state were reflected in the polar lipid composition (phospholipids, monogalactosyl diglycerides [MGDG], digalactosyl diglycerides). Probable light limitation during the fourth growth cycle may explain some of the changes in fatty acid composition at the MGDG level. In all cases, transient saturation-desaturation processes of the membrane lipids seems at the heart of the acclimation dynamics

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of variable nutrient supply on fatty acid composition of phytoplankton grown in an enclosed experimental ecosystem

Mayzaud¹,

Claustre²,

Augier³

1990

Mar. Ecol. Prog. Ser.

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“…Such differences in the quantitative proportions can result from different growth conditions regarding nutrients, temperature and irradiance (Ackman et al 1968;Mortensen et al 1988;Parrish et al 1991). Decreasing temperature is one of the main factors which can shift the FA composition of membrane lipids from MUFA dominated to a more PUFA dominated composition (Mortensen et al 1988;Thompson et al 1992;Jiang and Gao 2004) to maintain the membrane fluidity (Murata and Los 1997). In N. cf.…”

Section: Fatty Acid Compostitionmentioning

confidence: 99%

Effects of prolonged darkness and temperature on the lipid metabolism in the benthic diatom Navicula perminuta from the Arctic Adventfjorden, Svalbard

et al. 2017

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which could consequently lead to a depletion of this energy reserves before the end of the polar night. On the other hand, the membrane building phospho-and glycolipids remained unchanged during the 8 weeks darkness, indicating still intact thylakoid membranes. These results explain the shorter survival times of polar diatoms with increasing water temperatures during prolonged dark periods. Abstract The Arctic represents an extreme habitat for phototrophic algae due to long periods of darkness caused by the polar night (~4 months darkness). Benthic diatoms, which dominate microphytobenthic communities in shallow water regions, can survive this dark period, but the underlying physiological and biochemical mechanisms are not well understood. One of the potential mechanisms for long-term dark survival is the utilisation of stored energy products in combination with a reduced basic metabolism. In recent years, water temperatures in the Arctic increased due to an ongoing global warming. Higher temperatures could enhance the cellular energy requirements for the maintenance metabolism during darkness and, therefore, accelerate the consumption of lipid reserves. In this study, we investigated the macromolecular ratios and the lipid content and composition of Navicula cf. perminuta Grunow, an Arctic benthic diatom isolated from the microphytobenthos of Adventfjorden (Svalbard, Norway), over a dark period of 8 weeks at two different temperatures (0 and 7 °C). The results demonstrate that N. perminuta uses the stored lipid compound triacylglycerol (TAG) during prolonged dark periods, but also the pool of free fatty acids (FFA). Under the enhanced temperature of 7 °C, the lipid resources were used significantly faster than at 0 °C, Keywords

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“…Certain FAs are also precursors of eicosanoids, the latter being involved in various physiological functions, including osmoregulation and immune responses (George et al 2008). Previous studies have shown that plankton lipids can be affected by N and P (Shifrin and Chisholm 1981;Jó nasdó ttir 1994;Klein Breteler et al 2005), Si (Shifrin and Chisholm 1981;Mortensen et al 1988), light conditions (Shifrin and Chisholm 1981;Renaud et al 1991), temperature (Mortensen et al 1988), and organic contaminants (Fisher and Schwarzenbach 1978). There are very few studies that have evaluated the influence of metal nutrients on the lipid composition of marine organisms (Nechev et al 2006).…”

mentioning

confidence: 99%

Influence of iron on fatty acid and sterol composition of marine phytoplankton and copepod consumers

Chen

Wakeham

Fisher

2011

Limnology & Oceanography

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We conducted laboratory experiments to determine the influence of iron on the fatty acid (FA) and sterol composition of the diatom Thalassiosira oceanica, the cryptophyte Rhodomonas salina, the prymnesiophyte Isochrysis galbana, and the copepod Acartia tonsa that fed on these algae. Algal cells were cultured with no added Fe or with Fe added at 100 nmol L 21 . Fe-deficient culture medium resulted in lower growth rates, cell volumes, and photosynthetic efficiencies for all algal species. The total mass of FA per cell was 2.2-3

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Fatty acid and elemental composition of the marine diatom Chaetoceros gracilis Schütt. Effects of silicate deprivation, temperature and light intensity

Cited by 115 publications

References 26 publications

Effect of variable nutrient supply on fatty acid composition of phytoplankton grown in an enclosed experimental ecosystem

Effect of variable nutrient supply on fatty acid composition of phytoplankton grown in an enclosed experimental ecosystem

Effects of prolonged darkness and temperature on the lipid metabolism in the benthic diatom Navicula perminuta from the Arctic Adventfjorden, Svalbard

Influence of iron on fatty acid and sterol composition of marine phytoplankton and copepod consumers

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