Fatty acid analogue accumulation: a marker of myocyte viability in ischemic-reperfused myocardium.

Miller, D. Douglas; Gill, John B.; Livni, Elijahu; Elmaleh, David R.; Aretz, Thomas; Boucher, Charles A.; Strausś, H. William

doi:10.1161/01.res.63.4.681

Cited by 57 publications

(23 citation statements)

References 40 publications

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“…Some discrepancies remain about the turnover of this tracer into complex lipid pools, that is either free FAs (Humbert et al 1989b(Humbert et al , 1990 or triglycetides (Ambrose et al 1987 a;Kawamura et al 1988) form. However, whatever this turnover, the radioactivity value likely corresponds to the extent of I M P P A uptake, and the changes in this uptake seem to be related to the extent of physiological changes occurring in the myocardium, as shown also in other studies (Yamamoto et al 1986;Miller et al 1988;Kurata et al 1989;Kawamura et al 1988).…”

Section: Discussionsupporting

confidence: 58%

“…The other slice of each pair was used to estimate necrotic foei by TTC histochemical staining (Cox et al 1986): slices were incubated in 0.37 mM solution of TTC for 30 min and then rinsed (Miller et al 1988). This staining with TTC washed out 2°1T1, whereas labelled FA analogue remained trapped in the cells: this slice was used for the estimation of labelled FA analogue by QARG.…”

Section: Isoprenaline Treatment and Administration Of Radioactive Promentioning

confidence: 99%

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Evaluation of cellular viability by quantitative autoradiographic study of myocardial uptake of a fatty acid analogue in isoproterenol-induced focal rat heart necrosis

et al. 1991

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Previous studies led us to hypothesize that a fatty acid analogue, 15-p-iodophenyl-beta-methyl pentadecanoic acid (IMPPA or BMIPP), which is taken up but not quickly metabolized by heart cells, would be a more suitable tracer of cellular viability than thallium-201. Biodistribution studies of 1-14C-IMPPA in conscious, freely moving rats showed that the concentration ratio of radioactivity in the heart with respect to the blood was about 8 for at least 60 min after intravenous administration, permitting its use as a putative tracer in these conscious, freely moving rats. Thereafter, the myocardial uptake of 14C-IMPPA was studied in isoproterenol-treated rats (daily treatment for 10 days in order to induce cardiac hypertrophy and necrotic foci) with respect to control ones. Comparison of myocardial localizations by quantitative autoradiography of the uptake of 201Tl and 14C-IMPPA with that of triphenyltetrazolium chloride (TTC) staining enabled comparative evaluation of nutritional blood flow, localization and uptake of 14C-IMPPA and necrotic foci size. Distributions of 14C-IMPPA and 201Tl in control rats' hearts were homogeneous, like TTC staining. In infarcted hearts, areas of decreased 14C-IMPPA uptake were nearly the same (100% +/- 5%) as those unstained by TTC. These areas were larger than those showing a decrease in thallium uptake (about 70% +/- 5% of the total scar size). Therefore, IMPPA seems to be a more accurate and sensitive indicator of necrosis localization compared with thallium. It may be a useful agent for assessment of myocardial viability by single photon emission tomography (SPET) imaging.

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Section: Discussionsupporting

confidence: 58%

Section: Isoprenaline Treatment and Administration Of Radioactive Promentioning

confidence: 99%

Evaluation of cellular viability by quantitative autoradiographic study of myocardial uptake of a fatty acid analogue in isoproterenol-induced focal rat heart necrosis

et al. 1991

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“…This em phasizes that the subendocardial zone is the area most susceptible to an ischemia-reperfusion insult. Accordingly, TTC may underesti mate the ultimate extent of myocardial dam age [10], and therefore ultrastructural assess ment was considered to be the most reliable method. Biopsies were performed in the bed perfused by the LAD.…”

Section: Discussionmentioning

confidence: 99%

Effects of Cloricromene on Ischemia-Reperfusion Myocardial Damage in the Rabbit

Milei¹,

Ferreira²,

Grana³

et al. 1992

Cardiology

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Cloricromene, a compound with several biological activities which suggest a therapeutic role in thrombosis or ischemic disease, has been studied for its effects on the extension of myocardial damage and the production of oxygen free radicals during periods of ischemia and reperfusion. In twenty rabbits the left anterior descending coronary artery (LAD) was occluded and cloricromene (3.6 µg /kg /min; n = 10) or placebo (n = 10) were continuously infused. After 50 min the artery was reopened and after 20 min of reperfusion a biopsy was obtained from the anterior wall of the heart in the LAD area and from the posterior wall (control myocardium) prior to sacrifice. Both samples were used for chemiluminescence measurement (free-radical production) and ultrastructural studies. In the placebo group all rabbits showed ST-segment changes during ischemia and reperfusion arrhythmias, while in the cloricromene group there were transient ST elevations in 4 animals which reverted at higher infusion rates of the drug. The chemiluminescence values were 18,017 ± 1,956 and 8,583 ± 918 cpm/mg protein (p < 0.001) in the anterior and posterior walls of the left ventricle, respectively, for the placebo group, and 7,767 ± 992 and 8,333 ± 832 cpm/mg protein (NS), respectively, for the cloricromene group. The ratio between the anterior and posterior wall was 2.27 ± 0.37 for the placebo group versus 0.95 ± 0.11 for the cloricromene group (p < 0.001). In ultrastructural studies, the anterior wall in the placebo group showed irreversible myocyte injury and infarction as well as mitochondrial damage. Samples from the cloricromene-treated group showed, in general, preservation of myocyte architecture or minor signs of injury. These results illustrate clearly the protective effect of cloricromene during damage induced by ischemia and reperfusion in the rabbit.

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“…These baseline myocardial blood flows in the partial stenosis group represent the ruthenium-103 microsphere myocardial blood flow data obtained prior to occlusion and drug infusion. Microsphere myocardial blood flows greater than 0.5 ml/min/g are considered normal in our laboratory, although normal flows frequently run closer to 1 ml/min/ g ( 18). Experimental differences in the bolus injection of microspheres, the reference blood withdraw rate, and the coronary perfusion pressure in the anesthetized dogs may have contributed to the above range in "normal" myocardial blood flow.…”

Section: Effect Of Lad Stenosis: With and Without Dipyridamolementioning

confidence: 95%

MRI detection of myocardial perfusion changes by gadolinium‐DTPA infusion during dipyridamole hyperemia

Miller

Holmvang

Gill

et al. 1989

Magnetic Resonance in Med

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To detect abnormal regional myocardial coronary flow reserve, serial spin-echo MR tomograms of four control dogs and six with a partial balloon occlusion of the left anterior descending (LAD) coronary artery were acquired before and after dipyridamole infusion and during the paramagnetic effect of a bolus plus infusion of gadolinium-DTPA. Microsphere myocardial blood flow was measured for correlation with serial regional changes in MR signal intensity. Significant difference in gadolinium-enhanced MR signal intensity existed between the hypoperfused LAD and the hyperemic left circumflex perfusion beds (46 +/- 22% vs 78 +/- 29% above baseline, respectively; P less than 0.05). The percentage changes in gadolinium-enhanced MR signal and microsphere myocardial blood flow were significantly correlated (r2 = 0.93).

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Fatty acid analogue accumulation: a marker of myocyte viability in ischemic-reperfused myocardium.

Abstract: A cute reperfusion therapy is widely practiced in the setting of evolving myocardial infarction. 12 However, its effect on myocardial salvage and, hence, prognosis is variable.3 -5

Cited by 57 publications

References 40 publications

Evaluation of cellular viability by quantitative autoradiographic study of myocardial uptake of a fatty acid analogue in isoproterenol-induced focal rat heart necrosis

Evaluation of cellular viability by quantitative autoradiographic study of myocardial uptake of a fatty acid analogue in isoproterenol-induced focal rat heart necrosis

Effects of Cloricromene on Ischemia-Reperfusion Myocardial Damage in the Rabbit

MRI detection of myocardial perfusion changes by gadolinium‐DTPA infusion during dipyridamole hyperemia

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