Fate of Shed Mast Cell Granules.

Higginbotham, R. D.; Dougherty, Thomas F.; Jee, W.S.S.

doi:10.3181/00379727-92-22445

Cited by 63 publications

(22 citation statements)

References 2 publications

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“…This finding is in agreement with a recent study on the immunologic release of heparin from rat mast cells in which the majority of released 3SS-labelled heparin was found by differential centrifugation to be granule associated (35). It is also of interest in this connection to recall previous qualitative microscopic in vivo observations that shed, extracellular mast cell granules retain basophilia and metachromasia for a long time (20).…”

Section: Discussionmentioning

confidence: 99%

A Cytofluorometric Analysis of Polymer-Induced Mast Cell Secretion

Gustafsson¹,

Enerbäck²

1980

Pathobiology

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Quantitative cytofluorometry was used to study the mechanism of mast cell secretion induced by polymyxin B in vitro. We measured 5-hydroxytryptamine (5-HT) and heparin in mast cells and individual mast cell granules, extruded from the cells or obtained from the interior of the cells by micromanipulation. Mast cell granules of untreated control cells contained both 5-HT and heparin. After polymyxin stimulation both intracellular and extruded mast cell granules contained heparin, but some intracellular and all extruded granules lacked 5-HT. These results indicate that the mast cell amines may be released from granules while they are still within the cellular domain. The mast cell granules did not release heparin whether extruded from the cell or not. The release of heparin from mast cells was thus determined by the number of extruded granules. Our results further suggest that the granules first released are those situated in the periphery of the cell and contain the largest quantities of amines and heparin. In agreement with the granule measurements, fluorescence measurements on stimulated mast cells showed a dose-dependent decrease of both heparin and 5-HT, but the 5-HT content decreased proportionally more than that of heparin.

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Section: Discussionmentioning

confidence: 99%

A Cytofluorometric Analysis of Polymer-Induced Mast Cell Secretion

Gustafsson¹,

Enerbäck²

1980

Pathobiology

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“…These will all cause extracellular granules to appear in the vicinity of mast cells ; where an increase has been seen in the present study it is possible to see one or more of these factors at work. Smith and Lewis (1958), Higginbotham, Dougherty andJee (1956) andFawcett (1955) have shown that such extruded granules are taken up by macrophages and fibroblasts in the vicinity so that, if a single stimulus has caused extrusion of granules, none is found extracellularly after 24 hours. Some of the phagocytes, however, contain so many granules that they resemble normal mast cells ; such granules are soon destroyed.…”

Section: Resultsmentioning

confidence: 99%

Effects of carcinogen and cortisone on mast cells in the hamster cheek pouch.

Smith¹

1967

Br J Cancer

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“…Riley suggests th a t these cells then produce mucopolysaccharides and some how these arc converted to extracellular ground substance which is later broken down and fed back into m ast cells. This theory is based on his own observations and those of Maximow [1904], Rrodersen [1928], Higginbotham et al [1956], and Smith and Lewis [1958].…”

Section: Discussionmentioning

confidence: 99%