A Cytofluorometric Analysis of Polymer-Induced Mast Cell Secretion

Gustafsson, Bertil; Enerbäck, Lennart

doi:10.1159/000162970

Cited by 5 publications

(25 citation statements)

References 16 publications

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“…In a recent investigation we used these methods for quantitation of polymyxin-in duced mast cell secretion of 5-HT and hep arin from mast cells and individual mast cell granules [17]. We found that heparin was not released from individual granules.…”

Section: Introductionmentioning

confidence: 90%

Cytofluorometric Analysis of Anaphylactic Secretion of 5-Hydroxytryptamine and Heparin from Rat Mast Cells

Gustafsson¹

1980

Int Arch Allergy Immunol

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Actively sensitized rat mast cells were stimulated to secretion in vitro by means of an anaphylactic IgE antigen reaction or with polymyxin B. Release of 5-hydroxytryptamine (5-HT) and heparin from the cells and from individual granules, extruded or displaced from stimulated cells with micromanipulation, was quantitated cytofluorometrically. Several similarities in action between the two secretagogues were found. All extruded and some intracellular granules from stimulated cells lacked 5-HT, and no heparin was released from the granules. Heparin secretion from mast cells was due to granule extrusion alone. Further, the proportions between 5-HT and heparin released from mast cells indicated that amine release, too, is mainly, but not exclusively, associated with overt granule exocytosis. The results suggested that the first extruded granules are the richest in both 5-HT and heparin. Antigen-induced secretion was not as vigorous as that induced by polymyxin and, unlike the latter, followed after a short lag of about 20 sec.

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Section: Introductionmentioning

confidence: 90%

Cytofluorometric Analysis of Anaphylactic Secretion of 5-Hydroxytryptamine and Heparin from Rat Mast Cells

Gustafsson¹

1980

Int Arch Allergy Immunol

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“…Measurement of the heparin content of individual granules extruded from mast cells as a result of a secretory process or obtained from the inte rior of the cell by micromanipulation produced no evidence for release of heparin from the granular ma trix [Gustavsson and Enerback, 1980]. Release of he parin can therefore be taken as a direct measure of re lease of granules.…”

Section: Discussionmentioning

confidence: 94%

“…1972a;Andersson et al, 1973;Gustavsson and Enerback, 1980], Granules in contact with the extracellular environment, wheth er retained in the cell or not, have an altered morphol ogy probably related to amine release [Bloom and Haegermark, 1965;Lagunoff 1973;Andersson and Uvnas, 1975]. It is still unsettled whether selective amine release can take place without accompanying exocytosis of granules.…”

Section: Discussionmentioning

confidence: 99%

“…It is extremely rapid, starting with a series of membrane fusions through which in dividual granules are brought into communication with the extracellular milieu, and terminating in the extrusion of the granules from the cell. The mast cell amines are thought to be released by a simple ion-ex change process which may take place when the gran ules are still situated within the cellular domains [Andersson et al, 1973;Gustavsson and Enerback. 1980].…”

Section: Introductionmentioning

confidence: 99%

“…1980]. Heparin does not appearto be released from the gran ular matrix in the early phase of the secretory re sponse [Gustavsson and Enerback, 1980], We have recently reported the use of the cationic dye berberine as a vital stain for demonstrating in tracellular granules in contact with the extracellular environment [Berlin and Enerback, 1983]. Vital ber berine staining was combined with cytofluorometric heparin quantitation by berberine binding to fixed cells [Enerback.…”

Section: Introductionmentioning

confidence: 99%

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Mast Cell Secretion

Berlin

Enerbäck

1984

Int Arch Allergy Immunol

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Mast cell secretion is closely associated with extrusion of granules from the cell, and results in rapid release of both amines and heparin. Amines may also be released from intracellular granules communicating with the extracellular environment through channels formed by membrane fusions. We have recently reported the use of the fluorescent, cationic dye berberine as a vital stain marking amine-releasing granules located within the domains of the cell. The present report concerns early membrane events and the fate of such granules after termination of the secretory process. Purified mast cells were stimulated to a moderate secretory response by a low concentration of polymyxin B. The cells were then kept in a medium without the secretagogue. The fluorescence intensity of individual cells after vital berberine staining (reflecting the number of intracellular granules in contact with the extracellular environment) decreased rapidly after terminated mast cell secretion. The heparin content (reflecting the total number of intracellular granules) and the histamine content of the cells, both reduced by the secretory process, remained constant over a period of 1 h after terminated mast cell secretion. These findings suggest that mast cell membranes are rapidly restored after secretion, permitting selective amine release without accompanying release of heparin or other matrix components.

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Compound 48/80 and substance P induced release of histamine and serotonin from rat peritoneal mast cells

Irman-Florjanc

Erjavec

1983

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The effect of substance P and compound 48/80 on histamine and serotonin release from not isolated and isolated mast cells have been compared in experiments in vitro. The response of not isolated and isolated mast cells were virtually identical. The release of both amines, in response to 48/80 and substance P, was dose-dependent. The percentage of histamine released by 48/80 was significantly higher than the percentage of serotonin, the difference being higher at lower concentrations of compound 48/80 after 15 min of incubation. Substance P also showed a tendency to higher efficiency for histamine than for serotonin release. In contrast to 48/80, the dose-response curves for histamine and serotonin release were parallel. These results support the view that the ratio between histamine and serotonin release depends on the liberator used. They also showed that this ratio can depend on the concentration of the agent inducing secretion. The results indicate that substance P as well as 48/80 act rather selectively as histamine liberators and that there is some difference in releasing properties of 48/80 and substance P.

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A Cytofluorometric Analysis of Polymer-Induced Mast Cell Secretion

Cited by 5 publications

References 16 publications

Cytofluorometric Analysis of Anaphylactic Secretion of 5-Hydroxytryptamine and Heparin from Rat Mast Cells

Cytofluorometric Analysis of Anaphylactic Secretion of 5-Hydroxytryptamine and Heparin from Rat Mast Cells

Mast Cell Secretion

Compound 48/80 and substance P induced release of histamine and serotonin from rat peritoneal mast cells

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