Fatal Algaemia in Patient with Chronic Lymphocytic Leukemia

Lanotte, Philippe; Baty, Gaëlle; Sénécal, Delphine; Dartigeas, Caroline; Bailly, Éric; Duong, Thanh Haï; Chandenier, J.; Goudeau, Alain

doi:10.3201/eid1507.090373

Cited by 14 publications

(6 citation statements)

References 8 publications

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“…For the identification of Prototheca spp., an alternative to all methods mentioned above is DNA sequencing, most commonly performed within the ribosomal operon. Indeed, PCR amplification followed by sequencing of the small-subunit (SSU) rRNA gene (18S rRNA), ITS region, and/or large-subunit (LSU) rRNA gene (28S rRNA) portions has been a common strategy for species delineation also in the clinical setting (4,(35)(36)(37)(38)(39)(40)(41) and for inference of phylogenetic and evolutionary relationships within and beyond the Prototheca genus (18,19,23,24,42,43). What hampers this approach from becoming a routine diagnostic tool are mainly time and financial constraints, especially for large-scale investigations.…”

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confidence: 99%

cytb as a New Genetic Marker for Differentiation of Prototheca Species

et al. 2018

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Achlorophyllous unicellular microalgae of the genus (, ) are the only known plants that cause infections in both humans and animals, collectively referred to as protothecosis. Human protothecosis, most commonly manifested as cutaneous, articular, and disseminated disease, is primarily caused by, followed by and, sporadically, by and In veterinary medicine, however, is a major pathogen responsible for bovine mastitis, which is a predominant form of protothecal disease in animals. Historically, identification of spp. has relied upon phenotypic criteria; these were later replaced by molecular typing schemes, including DNA sequencing. However, the molecular markers interrogated so far, mostly located in the ribosomal DNA (rDNA) cluster, do not provide sufficient discriminatory power to distinguish among all spp. currently recognized. Our study is the first attempt to develop a fast, reliable, and specific molecular method allowing identification of all spp. We propose the mitochondrial gene as a new and robust marker for diagnostics and phylogenetic studies of the algae. The gene displayed important advantages over the rDNA markers. Not only did the gene have the highest discriminatory capacity for resolving all species, but it also performed best in terms of technical feasibility, understood as ease of amplification, sequencing, and multiple alignment analysis. Based on the species-specific polymorphisms in the partial gene, we developed a fast and straightforward PCR-restriction fragment length polymorphism (RFLP) assay for identification and differentiation of all species described so far. The newly proposed method is advocated to be a new gold standard in diagnostics of protothecal infections in human and animal populations.

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confidence: 99%

cytb as a New Genetic Marker for Differentiation of Prototheca Species

et al. 2018

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“…Although numerous cases of Prototheca infection in immunocompetent hosts with or without a history of trauma have been reported , immunosuppression is increasingly recognized as a risk factor, as it has been reported in patients with acquired immunodeficiency syndrome (AIDS) , diabetes mellitus , chronic steroid use , steroid and cyclosporine use for myasthenia gravis , belimumab and rituximab use for systemic lupus erythematous , chemotherapy for solid or hematologic malignancy , hematopoietic stem cell transplantation (HSCT) and solid organ transplantation , and infliximab therapy for graft‐versus‐host disease after HSCT . A case of breakthrough protothecosis during long‐term voriconazole therapy for pulmonary aspergillosis after HSCT was also reported .…”

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confidence: 99%

Protothecal bursitis after simultaneous kidney/liver transplantation: a case report and review

Ramírez

Nieto-Ríos

Ocampo-Kohn

et al. 2016

Transplant Infectious Dis

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Solid organ transplantation is an accepted therapy for end-stage diseases of the kidneys, liver, heart, and lungs. Unfortunately, transplantation is associated with infectious complications. Here, we present a case report of Prototheca wickerhamii olecranon bursitis and review all of the cases in solid organ transplant (SOT) recipients published in the literature to date. In our patient, the infection resolved with surgical therapy and limited antifungal therapy, and no symptoms have recurred over 24 months of follow-up. A review of the literature suggests that 50% of SOT recipients with Prototheca infection present with disseminated infection, and the overall mortality is 75%. More studies are required to determine the optimal management of protothecosis in this population.

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“…Prevalence of protothecosis is low: 140 human cases have been reported so far [6]. Peritonitis is very unusual.…”

Section: A N S W E R T O T H E P H O T O Q U I Zmentioning

confidence: 99%