Fat balance and hepatic mitochondrial function in response to fat feeding in mature rats

Iossa, Susanna; Lionetti, Lillà; Mollica, Maria Pina; Barletta, Antonio; Liverini, Giovanna

doi:10.1038/sj.ijo.0801041

Cited by 9 publications

(9 citation statements)

References 38 publications

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“…In fact, the effect of the high‐fat lard diet to induce the increase in mitochondrial respiration capacity is evident when using nicotinamide adenine dinucleotide‐linked or lipid substrates, but not with flavin adenine dinucleotide‐linked substrate. This result is different from those previously reported in rats fed a high‐fat diet ad libitum (29), when no differences in hepatic mitochondrial oxidative capacity was found after 2 weeks of dietary treatment. This increase in hepatic mitochondrial respiratory capacity during high‐lard refeeding can be explained by the enhanced plasma FFA found in HF‐L rats.…”

Section: Discussioncontrasting

confidence: 99%

Hepatic Mitochondrial Energetics During Catch‐Up Fat With High‐Fat Diets Rich in Lard or Safflower Oil

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et al. 2012

Obesity

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We have investigated whether altered hepatic mitochondrial energetics could explain the differential effects of high‐fat diets with low or high ω6 polyunsaturated fatty acid content (lard vs. safflower oil) on the efficiency of body fat recovery (catch‐up fat) during refeeding after caloric restriction. After 2 weeks of caloric restriction, rats were isocalorically refed with a low‐fat diet (LF) or high‐fat diets made from either lard or safflower oil for 1 week, and energy balance and body composition changes were assessed. Hepatic mitochondrial energetics were determined from measurements of liver mitochondrial mass, respiratory capacities, and proton leak. Compared to rats refed the LF, the groups refed high‐fat diets showed lower energy expenditure and increased efficiency of fat gain; these differences were less marked with high‐safflower oil than with high‐lard diet. The increase in efficiency of catch‐up fat by the high‐fat diets could not be attributed to differences in liver mitochondrial activity. By contrast, the lower fat gain with high‐safflower oil than with high‐lard diet is accompanied by higher mitochondrial proton leak and increased proportion of arachidonic acid in mitochondrial membranes. In conclusion, the higher efficiency for catch‐up fat on high‐lard diet than on LF cannot be explained by altered hepatic mitochondrial energetics. By contrast, the ability of the high‐safflower oil diet to produce a less pronounced increase in the efficiency of catch‐up fat may partly reside in increased incorporation of arachidonic acid in hepatic mitochondrial membranes, leading to enhanced proton leak and mitochondrial uncoupling.

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Section: Discussioncontrasting

confidence: 99%

Hepatic Mitochondrial Energetics During Catch‐Up Fat With High‐Fat Diets Rich in Lard or Safflower Oil

Crescenzo

Bianco

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et al. 2012

Obesity

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“…In agreement, we have found that when a high-fat diet was given to adult rats, an increase in body lipid content was found. 37 In conclusion, our present results indicate that in rats fed a high-fat diet skeletal muscle contributes to the increased whole body lipid utilization, through an increase in lipid oxidative capacity associated with a decrease in energy coupling.…”

Section: Discussionsupporting

confidence: 57%

Skeletal muscle oxidative capacity in rats fed high-fat diet

et al. 2002

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OBJECTIVE: To investigate whether young rats respond to high-fat feeding through changes in energy efficiency and fuel partitioning at the level of skeletal muscle, to avoid obesity development. In addition, to establish whether the two mitochondrial subpopulations, which exist in skeletal muscle, ie subsarcolemmal and intermyofibrillar, are differently affected by high-fat feeding. DESIGN: Weaning rats were fed a low-fat or a high-fat diet for 15 days. MEASUREMENTS: Energy balance and lipid partitioning in the whole animal. State 3 and state 4 oxygen consumption rates in whole skeletal muscle homogenate. State 3 and state 4 oxygen consumption rates, membrane potential and uncoupling effect of palmitate in subsarcolemmal and intermyofibrillar mitochondria from skeletal muscle. RESULTS: Rats fed a high-fat diet showed an increased whole body lipid utilization. Skeletal muscle NAD-linked and lipid oxidative capacity significantly increased at the whole-tissue level, due to an increase in lipid oxidative capacity in subsarcolemmal and intermyofibrillar mitochondria and in NAD-linked activity only in intermyofibrillar ones. In addition, rats fed a highfat diet showed an increase in the uncoupling effect of palmitate in both the mitochondrial populations. CONCLUSIONS: In young rats fed a high-fat diet, skeletal muscle contributes to enhanced whole body lipid oxidation through an increased mitochondrial capacity to use lipids as metabolic fuels, associated with a decrease in energy coupling.

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“…Previous studies in other animals have shown conflicting results. Studies in humans and rats have shown that REE either increases (Cooling and Blundell, 1998;Iossa et al, 1999) or remains unchanged (Corbett et al, 1986;Abbott et al, 1990) with the consumption of a HF diet compared with a LF diet. Although the reason for this discrepancy is unclear, there is evidence suggesting that stage of growth may be an important factor in determining whether REE increases in response to a HF diet.…”

Section: Discussionmentioning

confidence: 99%

Resting energy expenditure and body composition of Labrador Retrievers fed high fat and low fat diets

Yoo

Ramsey

Havel

et al. 2006

Animal Physiology Nutrition

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A high dietary fat intake may be an important environmental factor leading to obesity in some animals. The mechanism could be either an increase in caloric intake and/or a decrease in energy expenditure. To test the hypothesis that high fat diets result in decreased resting energy expenditure (REE), we measured REE using indirect calorimetry in 10-adult intact male Labrador Retrievers, eating weight-maintenance high-fat (HF, 41% energy, average daily intake: 8018 +/- 1247 kJ/day, mean +/- SD) and low-fat (LF, 14% energy, average daily intake: 7331 +/- 771 kJ/day) diets for a 30-day period. At the end of each dietary treatment, body composition measurements were performed using dual-energy X-ray absorptiometry. The mean +/- SD REE was not different between diets (4940 +/- 361 vs. 4861 +/- 413 kJ/day on HF and LF diets respectively). Measurements of fat-free mass (FFM) and fat mass (FM) also did not differ between diets (FFM: 26.8 +/- 2.3 kg vs. 26.3 +/- 2.5 kg; FM: 3.0 +/- 2.3 vs. 3.1 +/- 1.5 kg on HF and LF diets respectively). In summary, using a whole body calorimeter, we found no evidence of a decrease in REE or a change in body composition on a HF diet compared with LF diet.

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Fat balance and hepatic mitochondrial function in response to fat feeding in mature rats

Cited by 9 publications

References 38 publications

Hepatic Mitochondrial Energetics During Catch‐Up Fat With High‐Fat Diets Rich in Lard or Safflower Oil

Hepatic Mitochondrial Energetics During Catch‐Up Fat With High‐Fat Diets Rich in Lard or Safflower Oil

Skeletal muscle oxidative capacity in rats fed high-fat diet

Resting energy expenditure and body composition of Labrador Retrievers fed high fat and low fat diets

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