Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare

Jensen, Tanja Lyholm; Kiersgaard, Maria Kristina; Mikkelsen, Lars Friis; Sørensen, Dorte Bratbo

doi:10.1177/0023677218824373

Cited by 9 publications

(2 citation statements)

References 42 publications

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“…5C). These higher levels reflect the normal nocturnal rise in leptin in response to feeding and activity (Jensen et al 2013(Jensen et al , 2019. Similarly, ghrelin levels were unchanged comparing fed and fasted animals at 6 and 12 h likely because this was at the start of their nocturnal activity and eating (Jensen et al 2013(Jensen et al , 2019 (Fig.…”

Section: Metabolic Changes With 6 and 12 H Fastingmentioning

confidence: 82%

Sex differences in somatotrope response to fasting: biphasic responses in male mice

Miles

Moreira

Allensworth-James

et al. 2020

Journal of Endocrinology

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Anterior pituitary somatotropes are important metabolic sensors responding to leptin by secreting growth hormone (GH). However, reduced leptin signals caused by fasting have not always correlated with reduced serum GH. Reports show that fasting may stimulate or reduce GH secretion, depending on the species. Mechanisms underlying these distinct somatotrope responses to fasting remain unknown. To define the somatotrope response to decreased leptin signaling we examined markers of somatotrope function over different time periods of fasting. Male and mice were fasted for 24 and 48 h, with female mice fasted for 24 h compared to fed ad libitum controls. Body weight and serum glucose were reduced in both males and females, but, unexpectedly, serum leptin was reduced only in males. Furthermore, in males serum GH levels showed a biphasic response with significant reductions at 24 h followed by a significant rise at 48 h, which coincided with the rise in serum ghrelin levels. In contrast, females showed an increase in serum GH at 24. We then explored mechanisms underlying the differential somatotrope responses seen in males and observed that pituitary levels of Gh mRNA increased, with no distinction between acute and prolonged fasting. By contrast, the Ghrhr mRNA (encoding GH releasing hormone receptor) and the Ghsr mRNA (encoding the ghrelin receptor) were both greatly increased at prolonged fasting times coincident with increased serum GH. These findings show sex differences in the somatotrope and adipocyte responses to fasting and support an adaptive role for somatotropes in males in response to multiple metabolic signals.

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Section: Metabolic Changes With 6 and 12 H Fastingmentioning

confidence: 82%

Sex differences in somatotrope response to fasting: biphasic responses in male mice

Miles

Moreira

Allensworth-James

et al. 2020

Journal of Endocrinology

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“…Having found the sensitization effect of glucose deprivation on selenite-induced cytotoxicity of cancer cells, we next asked whether the enhanced effect could be achieved in vivo. Fasting for 24 h results in a decrease in both plasma and intra-tumor glucose levels of mice [ 1 , 33 ]. Next, HCT116 and LLC xenograft models were employed to evaluate the anticancer effect of selenite/fasting described in Materials and Methods.…”

Section: Resultsmentioning

confidence: 99%

Glucose Limitation Sensitizes Cancer Cells to Selenite-Induced Cytotoxicity via SLC7A11-Mediated Redox Collapse

Chen

Zhang

Cao

et al. 2022

Cancers

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Combination of intermittent fasting and chemotherapy has been drawn an increasing attention because of the encouraging efficacy. In this study, we evaluated the anti-cancer effect of combination of glucose limitation and selenite (Se), a representative inorganic form of selenium, that is preferentially accumulated in tumors. Results showed that cytotoxic effect of selenite on cancer cells, but not on normal cells, was significantly enhanced in response to the combination of selenite and glucose limitation. Furthermore, in vivo therapeutic efficacy of combining selenite with fasting was dramatically improved in xenograft models of lung and colon cancer. Mechanistically, we found that SLC7A11 expression in cancer cells was up-regulated by selenite both in vitro and in vivo. The elevated SLC7A11 led to cystine accumulation, NADPH depletion and the conversion of cystine to cysteine inhibition, which in turn boosted selenite-mediated reactive oxygen species (ROS), followed by enhancement of selenite-mediated cytotoxic effect. The findings of the present study provide an effective and practical approach for increasing the therapeutic window of selenite and imply that combination of selenite and fasting holds promising potential to be developed a clinically useful regimen for treating certain types of cancer.

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Nutrition, feeding and animal welfare

Tobin,

Schuhmacher

2024

The UFAW Handbook on the Care and Management of Laboratory and Other Research Animals

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Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare

Cited by 9 publications

References 42 publications

Sex differences in somatotrope response to fasting: biphasic responses in male mice

Sex differences in somatotrope response to fasting: biphasic responses in male mice

Glucose Limitation Sensitizes Cancer Cells to Selenite-Induced Cytotoxicity via SLC7A11-Mediated Redox Collapse

Nutrition, feeding and animal welfare

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