Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina

Fusco, Luciano; Beraldo-Neto, Emidio; Francisco, Aleff Ferreira; Alfonso, Jorge; Soares, Andreimar Martins; Pimenta, Daniel C.; Leiva, Laura

doi:10.1016/j.toxcx.2020.100047

Cited by 8 publications

(11 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sample preparation is a critical step, required prior to any analytical step, particularly when the relative concentration range of the sample components is wide. One possible approach is to decomplex the sample by RP-HPLC [ 6 ]. In this work, we have chosen this approach in order to make it possible to identify both major toxins and minor components.…”

Section: Resultsmentioning

confidence: 99%

Proteomic characterization of Naja mandalayensis venom

Beraldo

Coelho

Sciani

et al. 2021

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Background: Naja mandalayensis is a spitting cobra from Myanmar. To the best of our knowledge, no studies on this venom composition have been conducted so far. On the other hand, few envenomation descriptions state that it elicits mainly local inflammation in the victims' eyes, the preferred target of this spiting cobra. Symptoms would typically include burning and painful sensation, conjunctivitis, edema and temporary loss of vision. Methods: We have performed a liquid-chromatography (C18-RP-HPLC) mass spectrometry (ESI-IT-TOF/MS) based approach in order to biochemically characterize N. mandalayensis venom. Results: A wide variety of three-finger toxins (cardiotoxins) and metallopeptidases were detected. Less abundant, but still representative, were cysteine-rich secretory proteins, L-amino-acid oxidases, phospholipases A 2 , venom 5'-nucleotidase and a serine peptidase inhibitor. Other proteins were present, but were detected in a relatively small concentration. Conclusion:The present study set the basis for a better comprehension of the envenomation from a molecular perspective and, by increasing the interest and information available for this species, allows future venom comparisons among cobras and their diverse venom proteins.

show abstract

Section: Resultsmentioning

confidence: 99%

Proteomic characterization of Naja mandalayensis venom

Beraldo

Coelho

Sciani

et al. 2021

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

show abstract

“…In addition, Oliveira and coauthors [47] described the antimicrobial activities against a diversity of microorganisms, the action modes and structure of new peptides from C. durissus. It is important to highlight that, over the years, studies by Brazilian researchers have also shown that the study of the composition of snake venoms, including peptides, can be applied beyond pharmacology, for example, to understand the evolutionary relationships between different snake species and their distribution pattern throughout evolution [48]. About these applications, currently, the development of biopesticides for agricultural use from snake venom has also been studied [11].…”

Section: Temporal and Geographical Aspectmentioning

confidence: 99%

Studies about Snake Peptides: a Review about Brazilian Contribution

Assis

Bittar

Amorim

et al. 2022

Braz. arch. biol. technol.

View full text Add to dashboard Cite

The peptides present in snake venoms are studied because of their properties, constitutions, mechanisms of action and pharmacological potential. Recognizing this potential, the present study reports the contributions of Brazilian researchers about snake peptides, between 1975 and July 2020. Thus, this study serves as a basis for monitoring this theme in Brazil, referring to the trends and patterns of scientific production. The results indicated a significant increase in Brazilian scientific production over the years, highlighting institutions located in São Paulo state as, the Butantan Institute and University of São Paulo. The main journal where Brazilian authors publish their research is the Toxicon Journal. Collaborative networks were identified between Brazilian institutions and among other countries with Brazil. The most investigated species are found in brazilian territory, mainly those belonging to the genus Bothrops and the species Crotalus durissus. The increase in studies about this theme in Brazil is evident, however, a gap is highlighted for states outside the southeastern region and for other venomous species.

show abstract

“…Proteins account for approximately 90% of the dry weight of snake venom 4,21,29,31,32 . C. d. terrificus (Cdt) venom is mainly composed of phospholipase A 2 (PLA 2 ), serinoproteases, hyaluronidases, L-amino acid oxidases, peptides, low molecular weight organic compounds, inorganic ions, and enzyme inhibitors 4,33 . The main toxins found in Cdt venom are Crotoxin, Convulxin, Gyroxin, and Crotamine 4,[6][7][8][34][35][36][37][38] .…”

Section: Crotalus Durissus Terrificus Venom: Composition General Phar...mentioning

confidence: 99%

“…Crotoxin represents approximately 40%-60% of the dry weight of the Cdt venom 4,8,19,33,36,[42][43][44] and is a potent neurotoxin formed by PLA 2 and crotapotin, forming a complex of high toxicity 4,8,32,38,[42][43][44][45][46][47][48][49][50][51][52][53][54] , and exhibits myotoxic, nephrotoxic, and cardiotoxic effects 4,37,38,43,44,46,48,50 . This neurotoxic action is mainly attributed to the inhibitory mechanism of acetylcholine release in presynaptic neurons 48,52,54 .…”

Section: Crotoxinmentioning

confidence: 99%