Fast Screening of Antibacterial Compounds from Fusaria

Søndergaard, Teis Esben; Fredborg, Marlene; Christensen, Ann-Maria Oppenhagen; Damsgaard, Sofie K; Kramer, Nikoline F; Giese, Henriette; Sørensen, Jens Laurids

doi:10.3390/toxins8120355

Cited by 39 publications

(36 citation statements)

References 42 publications

(55 reference statements)

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“…This was performed with a checkerboard analysis ( 31 ), which showed the MICs of bikaverin and beauvericin to be 60 (157 µM) and 240 (306 µM) µg/ml, respectively. The bikaverin MIC was higher than that reported for Escherichia coli of 65 µM ( 27 ), and the beauvericin MIC was higher than the reported 32 µM for Lactobacillus acidophilus and Staphylococcus aureus ( 28 ). The interaction between the metabolites was characterized as additive due to its fractional inhibitory concentration (FIC) index value of 0.708 ( Fig.…”

Section: Resultscontrasting

confidence: 56%

“…The induction of both bikaverin and beauvericin in response to the bacterial signal ralsolamycin, coupled with the accruement of bikaverin in chlamydospores, suggested a possible evolved function of these metabolites toward protection from the endosymbiotic bacteria. Indeed, both metabolites have been previously shown to be antibacterial ( 27 – 30 ).…”

Section: Resultsmentioning

confidence: 99%

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Conserved Responses in a War of Small Molecules between a Plant-Pathogenic Bacterium and Fungi

Spraker

Wiemann

Baccile

et al. 2018

mBio

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Small-molecule signaling is one major mode of communication within the polymicrobial consortium of soil and rhizosphere. While microbial secondary metabolite (SM) production and responses of individual species have been studied extensively, little is known about potentially conserved roles of SM signals in multilayered symbiotic or antagonistic relationships. Here, we characterize the SM-mediated interaction between the plant-pathogenic bacterium Ralstonia solanacearum and the two plant-pathogenic fungi Fusarium fujikuroi and Botrytis cinerea. We show that cellular differentiation and SM biosynthesis in F. fujikuroi are induced by the bacterially produced lipopeptide ralsolamycin (synonym ralstonin A). In particular, fungal bikaverin production is induced and preferentially accumulates in fungal survival spores (chlamydospores) only when exposed to supernatants of ralsolamycin-producing strains of R. solanacearum. Although inactivation of bikaverin biosynthesis moderately increases chlamydospore invasion by R. solanacearum, we show that other metabolites such as beauvericin are also induced by ralsolamycin and contribute to suppression of R. solanacearum growth in vitro. Based on our findings that bikaverin antagonizes R. solanacearum and that ralsolamycin induces bikaverin biosynthesis in F. fujikuroi, we asked whether other bikaverin-producing fungi show similar responses to ralsolamycin. Examining a strain of B. cinerea that horizontally acquired the bikaverin gene cluster from Fusarium, we found that ralsolamycin induced bikaverin biosynthesis in this fungus. Our results suggest that conservation of microbial SM responses across distantly related fungi may arise from horizontal transfer of protective gene clusters that are activated by conserved regulatory cues, e.g., a bacterial lipopeptide, providing consistent fitness advantages in dynamic polymicrobial networks.

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Section: Resultscontrasting

confidence: 56%

Section: Resultsmentioning

confidence: 99%

Conserved Responses in a War of Small Molecules between a Plant-Pathogenic Bacterium and Fungi

Spraker

Wiemann

Baccile

et al. 2018

mBio

View full text Add to dashboard Cite

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“…In contrast, 20 years ago, Becker et al [ 33 ] used culture techniques and observed no inhibition of bacterial growth including Lactobacillus acidophilus , Lactobacillus johnsoni , Lactobacillus plantarum and Lactobacillus reuteri . Finally, two independent studies [ 34 , 35 ] found no antibiotic effect of FB1, thus excluding an antimicrobial effect to explain a fumonisin-related shaping action on gut microbiota.…”

Section: Discussionmentioning

confidence: 99%

Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota

Mateos

Combes

Pascal

et al. 2018

Toxins

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Pigs are highly affected by dietary mycotoxin contamination and particularly by fumonisin. The effects of fumonisin on pig intestinal health are well documented, but little is known regarding its impact on gut microbiota. We investigate the effects of the fumonisin (FB1, 12 mg/kg feed) on the fecal microbiota of piglets (n = 6) after 0, 8, 15, 22, and 29 days of exposure. A control group of six piglets received a diet free of FB1. Bacterial community diversity, structure and taxonomic composition were carried out by V3–V4 16S rRNA gene sequencing. Exposure to FB1 decreases the diversity index, and shifts and constrains the structure and the composition of the bacterial community. This takes place as early as after 15 days of exposure and is at a maximum after 22 days of exposure. Compared to control, FB1 alters the ecological succession of fecal microbiota species toward higher levels of Lactobacillus and lower levels of the Lachnospiraceae and Veillonellaceae families, and particularly OTUs (Operational Taxonomic Units) of the genera Mitsuokella, Faecalibacterium and Roseburia. In conclusion, FB1 shifts and constrains age-related evolution of microbiota. The direct or indirect contribution of FB1 microbiota alteration in the global host response to FB1 toxicity remains to be investigated.

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“…In fact, 22 naphthoquinones from F. solani and F. oxysporum were examined by Baker and colleagues, of which 12 were antimicrobial against Streptococcus pyogenes and 15 were active against Staphylococcus aureus [ 20 ]. Aurofusarin was shown to inhibit probiotic bacteria, which might shift the gut microbes to a more harmful constitution causing illness and diarrhea [ 21 ]. Also, aurofusarin is cytotoxic, genotoxic, and induces oxidative stress in human colon cells [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum

Westphal

Wollenberg

Herbst

et al. 2018

Toxins

Self Cite

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There is an increasing demand for products from natural sources, which includes a growing market for naturally-produced colorants. Filamentous fungi produce a vast number of chemically diverse pigments and are therefore explored as an easily accessible source. In this study we examine the positive regulatory effect of the transcription factor AurR1 on the aurofusarin gene cluster in Fusarium graminearum. Proteomic analyses showed that overexpression of AurR1 resulted in a significant increase of five of the eleven proteins belonging to the aurofusarin biosynthetic pathway. Further, the production of aurofusarin was increased more than threefold in the overexpression mutant compared to the wild type, reaching levels of 270 mg/L. In addition to biosynthesis of aurofusarin, several yet undescribed putative naphthoquinone/anthraquinone analogue compounds were observed in the overexpression mutant. Our results suggest that it is possible to enhance the aurofusarin production through genetic engineering.

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Fast Screening of Antibacterial Compounds from Fusaria

Cited by 39 publications

References 42 publications

Conserved Responses in a War of Small Molecules between a Plant-Pathogenic Bacterium and Fungi

Conserved Responses in a War of Small Molecules between a Plant-Pathogenic Bacterium and Fungi

Fumonisin-Exposure Impairs Age-Related Ecological Succession of Bacterial Species in Weaned Pig Gut Microbiota

Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum

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