Fast Scanning Electron Microscopic and Light Microscopic Correlation of Paraffin Sections and Chromosome Spreads of Nickel-Induced Tumor

Hd, Geissinger; Pk, Basrur; Yamashiro, S.

doi:10.2307/3224917

Cited by 6 publications

(3 citation statements)

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“…The other sequence, LM examination before SEM, is also possible and not only on paraffin-embedded sections (Geissinger, 1971;Geissinger et al, 1973;Poh et al, 1971;Riddle et al, 1976;Crocker et al, 1980), but also on thicker sections, for example those made from freshly extracted teeth (Mortimer & Tranter, 1971;Hubbard, 1982). Paraffinembedded sections can be investigated in the light microscope without staining, using Nomarski differential-interference contrast (Geissinger, 1971;Geissinger et al, 1973) or with staining using conventional LM (Geissinger, 1971;Riddle et al, 1976;Crocker et al, 1980). Before SEM processing and examination can be performed the coverslips and the paraffin are removed.…”

Section: Sectionsmentioning

confidence: 99%

Techniques for combining light microscopy and scanning electron microscopy: a survey of the literature

Wouters

1987

Journal of Microscopy

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SUMMARY A survey of methods combining light microscopy and scanning electron microscopy is presented. A simple correlation is made when two preparations from adjacent parts of one specimen are investigated in two different microscopes. A more sophisticated method is the consecutive investigation of one specimen with two microscopes. A major problem in this method is the relocation of the area of interest. Several authors have presented solutions for this problem. It is preferable when one preparation is investigated in only one instrument, combining the two microscopical (LM and SEM) techniques, thus making relocation redundant.

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Section: Sectionsmentioning

confidence: 99%

Techniques for combining light microscopy and scanning electron microscopy: a survey of the literature

Wouters

1987

Journal of Microscopy

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“…Specimen preparation techniques for tissue sections on slides, either formalin- (Poh et al, 1971) or glutaraldehyde- (Geissinger, Basrur & Yamashiro, 1973) fixed, or of minerals (Wilding & Geissinger, 1973), have been worked out and used successfully for correlative microscopy. Stained or unstained microtome section and smears on standard microscope slides can be examined in a light microscope in transillumination using every existing LM-technique (bright-field, oblique, dark-field, phase contrast, Nomarski differential interference contrast, polarizing, quantitative interference, and fluorescence microscopy).…”

Section: U L T I P L E Use O F I N S T R U M E N T a T I O N A N D mentioning

confidence: 99%

“…The SEM in the reflective scanning mode (secondary electron, primary backscattered electron, photon, and X-ray emission) and in the transmissive mode (Swift & Brown, 1970) yields a different type of information regarding a specimen, at axial and lateral resolution which are basically governed by electron optical parameters. The correlation of these two types of information has been demonstrated to be useful (McDonald & Hayes, 1969;Geissinger, 1971Geissinger, , 1972Poh et al, 1971;Geissinger et al, 1973;Wilding & Geissinger, 1973).…”

Section: U L T I P L E Use O F I N S T R U M E N T a T I O N A N D mentioning

confidence: 99%

A precise stage arrangement for correlative microscopy for specimens mounted on glass slides, stubs or EM grids

Geissinger

1974

Journal of Microscopy

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Summary The instrumentation necessary for precise and fast correlation of images derived from a light optical microscope (LM) and a scanning electron microscope (SEM) operated in the reflective mode, is described. The specimens can be mounted on standard microscope slides (25 × 75 mm), SEM‐stubs (12 mm ø), or on transmission EM grids (3 mm ø). The instrumentation consists of two parts: an attachable precision stage for an LM, and an attachable slide carrier for the stage of an SEM. By taking into account the vernier readings of the stages of both microscopes (LM and SEM), identical particles in a specimen can be found instantaneously under either microscope. Therefore it is concluded that the use of this instrumentation in correlative microscopy (LM → SEM → LM) is time saving, and especially recommended on fragile biological specimens, which may deteriorate rapidly under the electron beam of an SEM.

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It's a Small, Small World

Guérin

Liv

Klumperman

2019

Correlative Imaging

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Fast Scanning Electron Microscopic and Light Microscopic Correlation of Paraffin Sections and Chromosome Spreads of Nickel-Induced Tumor

Cited by 6 publications

References 0 publications

Techniques for combining light microscopy and scanning electron microscopy: a survey of the literature

Techniques for combining light microscopy and scanning electron microscopy: a survey of the literature

A precise stage arrangement for correlative microscopy for specimens mounted on glass slides, stubs or EM grids

It's a Small, Small World

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