Fast Preparation of Hydroxyapatite/Superhydrophilic Vertically Aligned Multiwalled Carbon Nanotube Composites for Bioactive Application

Lobo, Anderson Oliveira; Corat, Marcus Alexandre Finzi; Ramos, Susana; Matsushima, Jorge Tadao; Granato, Alessandro E.C.; Soares, Cristina Pacheco; Corat, Evaldo José

doi:10.1021/la1034646

Cited by 53 publications

(67 citation statements)

References 47 publications

(94 reference statements)

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“…B o t h t h e n H A p a n d VA M W C N T-O 2 h a v e physicochemical properties (surface tension, surface free energy, ionic charge, and wettability) suitable for cell adhesion and proliferation 6 ; however, surface topography and cell protein production activity also interfere with the cell adhesion process 13 . In relation to surface topography, geometric structure is related to material roughness and porosity.…”

Section: Resultsmentioning

confidence: 99%

“…In the first 6 hours of contact with the biomaterial, cells begin the surface recognition process 6 . The biomimetic nHAp/VAMWCNT-O 2 nanocomposite surface, before and after 7 days of biomineralization, characterizes nanocomposite bioactivity.…”

Section: Resultsmentioning

confidence: 99%

“…Functionalization of the VAMWCNT tips by the incorporation of oxygen-containing groups to obtain the superhydrophilic character was performed in a pulsed-direct current plasma reactor with an oxygen flow rate of 1 sccm, at a pressure of 85 mTorr, -700 V, and with a frequency of 20 kHz 6 . The electrodeposition of the nHAp crystals on the superhydrophilic VAMCNT (VAMWCNT-O 2 ) films was performed using 0.042 mol.L (NH 4 ).2HPO 4 electrolytes (pH = 4.8).…”

Section: Methodsmentioning

confidence: 99%

“…The nanohydroxyapatite (nHAp) is a ceramic material that promotes bone growth due to its biocompatible and bioactive behavior. nHAp has application in bone regeneration because it can establish chemical bonds between the material and the bone tissue allowing cell proliferation due to its similarity to bone mineralized matrix 6 . In addition to the application of nHAp/VAMWCNT-O 2 as scaffolds, the process of biomineralization in biomaterials conducted by incubating the samples in SBF (ion concentrations similar to human extracellular fluid) assists the development of biological apatites on the surface of nHAp/VAMWCNT-O 2, improving in vitro adhesion and proliferation of human osteoblast cells (HOB) 7,8 .…”

Section: Introductionmentioning

confidence: 99%

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Calcification in vitro of biomineralizated nanohydroxyapatite/superydrophilic vertically aligned multiwalled carbon nanotube scaffolds

et al. 2013

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Nanocomposites based on superhydrophilic vertically aligned multi-walled carbon nanotubes (VAMWCNT-O 2 ) and nanohydroxyapatite (nHAp) are of great interest in bone regenerative medicine. The biomineralization using simulated body fluid (SBF) has been extensively studied to evaluate the bioactivity of biomaterials. Thus, the combination of nHAp and VAMWCNT-O 2 is attractive and promising. The aim of this study was to evaluate the in vitro calcification of nHAp/VAMWCNT-O 2 nanocomposites before and after the period of biomineralization in SBF. In vitro calcification of the extracellular matrix (ECM) of HOB cells in culture after 24 hours was investigated through the assay of alkaline phosphatase. These promising in vitro results validate biomineralized nHAp/VAMWCNT-O 2 as possible scaffolds for bone tissue regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Calcification in vitro of biomineralizated nanohydroxyapatite/superydrophilic vertically aligned multiwalled carbon nanotube scaffolds

et al. 2013

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“…[12][13][14][15][16] Combining MWCNTs and GO with HAp in its many forms (such as nano) represents an alternative to improve the chemical, physical, and biological characteristics of polymeric nanocomposites, as well as to generate materials that can mimic bone tissues. 11,[17][18][19] For example, Lahiri et al 20 demonstrated that MWCNTs were responsible for increasing the adhesion strength, and spreading and attachment of osteoblasts in HAp/MWCNT nanocomposites. Balani et al 21 reported on the improvement of the fracture toughness and crystallinity of the coating of HAp/MWCNT nanocomposites prepared by plasma spraying.…”

Section: Introductionmentioning

confidence: 99%

Graphene oxide/multi-walled carbon nanotubes as nanofeatured scaffolds for the assisted deposition of nanohydroxyapatite: characterization and biological evaluation

Rodrigues¹,

Leite²,

Cavalcanti³

et al. 2016

IJN

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Nanohydroxyapatite (nHAp) is an emergent bioceramic that shows similar chemical and crystallographic properties as the mineral phase present in bone. However, nHAp presents low fracture toughness and tensile strength, limiting its application in bone tissue engineering. Conversely, multi-walled carbon nanotubes (MWCNTs) have been widely used for composite applications due to their excellent mechanical and physicochemical properties, although their hydrophobicity usually impairs some applications. To improve MWCNT wettability, oxygen plasma etching has been applied to promote MWCNT exfoliation and oxidation and to produce graphene oxide (GO) at the end of the tips. Here, we prepared a series of nHAp/MWCNT-GO nanocomposites aimed at producing materials that combine similar bone characteristics (nHAp) with high mechanical strength (MWCNT-GO). After MWCNT production and functionalization to produce MWCNT-GO, ultrasonic irradiation was employed to precipitate nHAp onto the MWCNT-GO scaffolds (at 1–3 wt%). We employed various techniques to characterize the nanocomposites, including transmission electron microscopy (TEM), Raman spectroscopy, thermogravimetry, and gas adsorption (the Brunauer–Emmett–Teller method). We used simulated body fluid to evaluate their bioactivity and human osteoblasts (bone-forming cells) to evaluate cytocompatibility. We also investigated their bactericidal effect against Staphylococcus aureus and Escherichia coli . TEM analysis revealed homogeneous distributions of nHAp crystal grains along the MWCNT-GO surfaces. All nanocomposites were proved to be bioactive, since carbonated nHAp was found after 21 days in simulated body fluid. All nanocomposites showed potential for biomedical applications with no cytotoxicity toward osteoblasts and impressively demonstrated a bactericidal effect without the use of antibiotics. All of the aforementioned properties make these materials very attractive for bone tissue engineering applications, either as a matrix or as a reinforcement material for numerous polymeric nanocomposites.

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Osteoconductive Performance of Carbon Nanotube Scaffolds Homogeneously Mineralized by Flow‐Through Electrodeposition

Nardecchia

Serrano

Gutiérrez

et al. 2012

Adv Funct Materials

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The treatment of bone lesions, including fractures, tumor resection and osteoporosis, is a common clinical practice where bone healing and repair are pursued. It is widely accepted that calcium phosphate‐based materials improve integration of biomaterials with surrounding bone tissue and further serve as a template for proper function of bone‐forming cells. Within this context, mineralization on preformed substrates appears as an interesting and successful alternative for mineral surface functionalization. However, mineralization of “true” 3D scaffolds –in which the magnitude of the third dimension is within the same scale as the other two– is by no means a trivial issue because of the difficulty to obtain a homogeneous mineral layer deposited on the entire internal surface of the scaffold. Herein, a “flow‐through” electrodeposition process is applied for mineralization of 3D scaffolds composed of multiwall carbon nanotubes and chitosan. It is demonstrated that, irrespective of the experimental conditions used for electrodeposition (e.g., time, temperature and voltages), the continuous feed of salts provided by the use of a flow‐through configuration is the main issue if one desires to coat the entire internal structure of 3D scaffolds with a homogeneous mineral layer. Finally, mineralized scaffolds not only showed a remarkable biocompatibility when tested with human osteoblast cells, but also enhanced osteoblast terminal differentiation (as early as 7 days in calcifying media).

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Fast Preparation of Hydroxyapatite/Superhydrophilic Vertically Aligned Multiwalled Carbon Nanotube Composites for Bioactive Application

Cited by 53 publications

References 47 publications

Calcification in vitro of biomineralizated nanohydroxyapatite/superydrophilic vertically aligned multiwalled carbon nanotube scaffolds

Calcification in vitro of biomineralizated nanohydroxyapatite/superydrophilic vertically aligned multiwalled carbon nanotube scaffolds

Graphene oxide/multi-walled carbon nanotubes as nanofeatured scaffolds for the assisted deposition of nanohydroxyapatite: characterization and biological evaluation

Osteoconductive Performance of Carbon Nanotube Scaffolds Homogeneously Mineralized by Flow‐Through Electrodeposition

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