Fast microwave‐assisted extraction of rotenone for its quantification in seeds of yam bean (<i><scp>P</scp>achyrhizus</i> sp.)

Lautie, Emmanuelle; Rasse, Catherine; Rozet, Eric; Mourgues, Claire; Vanhelleputte, Jean-Paul; Quetin-Leclercq, Joëlle

doi:10.1002/jssc.201200347

Cited by 8 publications

(2 citation statements)

References 17 publications

(20 reference statements)

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“…Extraction of Rotenone and Cleanup Procedure. The microwave-assisted extraction (MAE) conditions were optimized in Lautie et al 24 MAE were performed in a closed-vessel system (MARS, CEM Corp., USA), and 20.0 mL of a 50:50 mixture of DCM/MeOH was placed in the glass extraction vessels with two stirring bars. The different vessels were placed into the extractor, which was programmed to make a cycle of 11 min at 55 °C, with the potency of the extractor set to 400 W. At the end of each extraction, the vessels were allowed to cool to 38 °C before opening.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Degradation of Rotenone in Yam Bean Seeds (Pachyrhizus sp.) through Food Processing

Catteau

Lautie

Koné

et al. 2013

J. Agric. Food Chem.

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The purpose of this research is to screen different processes that could potentially decrease or even eliminate rotenone, a toxic isoflavonoid, from Pachyrhizus seeds. Yam bean seeds have very interesting nutritional characteristics, especially their high protein and lipid contents, and could potentially increase food security in under-nourished populations. However, they contain rotenone, a natural molecule previously used as an insecticide inhibiting the respiratory mitochondrial chain. It was also proven to be toxic to mammals as chronic exposure leads to the development of Parkinson-like symptoms in rats. As the thermosensitivity of rotenone had been reported, this study tested different processes (drying, roasting, boiling, frying, alcohol extraction), tegument removal, and traditional Beninese culinary recipes. Rotenone was then quantified in end-products by a validated method, associating microwave extraction, solid phase extraction (SPE), and HPLC-UV. With these processes a rotenone removal of up to 80% was obtained. The most effective methods were the drying and roasting of the seeds and the maceration of their flour in local alcohol. Rotenone degradation and elimination were confirmed by cytotoxic assays, effectively inducing a decrease in sample toxicity.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Degradation of Rotenone in Yam Bean Seeds (Pachyrhizus sp.) through Food Processing

Catteau

Lautie

Koné

et al. 2013

J. Agric. Food Chem.

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“…seed oil has been done by using MAE. It revealed that MAE of 11 min was more efficient as compared to 8 h of conventional Soxhlet extraction . Due to dipole spin and ionic conductance (specific properties of microwaves on the matter), microwave heating is rapid and occurs in the sample, which provided results in quick extractions.…”

Section: Maementioning

confidence: 99%

Advanced analytical techniques for the extraction and characterization of plant-derived essential oils by gas chromatography with mass spectrometry

Waseem

Low

2015

J. Sep. Science

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In recent years, essential oils have received a growing interest because of the positive health effects of their novel characteristics such as antibacterial, antifungal, and antioxidant activities. For the extraction of plant-derived essential oils, there is the need of advanced analytical techniques and innovative methodologies. An exhaustive study of hydrodistillation, supercritical fluid extraction, ultrasound- and microwave-assisted extraction, solid-phase microextraction, pressurized liquid extraction, pressurized hot water extraction, liquid-liquid extraction, liquid-phase microextraction, matrix solid-phase dispersion, and gas chromatography (one- and two-dimensional) hyphenated with mass spectrometry for the extraction through various plant species and analysis of essential oils has been provided in this review. Essential oils are composed of mainly terpenes and terpenoids with low-molecular-weight aromatic and aliphatic constituents that are particularly important for public health.

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Efficient optimization of ultra‐high‐ performance supercritical fluid chromatographic separation of Rosa sericea by response surface methodology

Xia

et al. 2013

J of Separation Science

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An approach for rapid optimization of ultra-high-performance supercritical fluid chromatographic (UHPSFC) gradient by response surface methodology was developed for fast separation of complex crude extracts of the leaves of Rosa sericea. The optimization was performed with Box-Behnken designs and the multicriteria response variables were described using Derringer's desirability. Based on factorial design experiments, five factors were selected for Box-Behnken designs to optimize the UHPSFC conditions, which led to 46 experiments being performed within 8 h. An evaporative light-scattering detector (ELSD) was used, and quantitative analysis of main components in R. sericea samples was employed to evaluate the statistical significance of the parameters on UHPSFC-ELSD analytes response. The results indicated that the optimized UHPSFC-ELSD method is very sensitive with LODs and LOQs below 1.19 and 4.55 μg/mL, respectively. The overall intra- and interday variations were less than 3.91 and 6.41%, respectively. The recovery of the method ranged from 95.66 to 104.22%, with RSD < 5.91%. This newly developed UHPSFC-ELSD method was demonstrated to be fast and sensitive in analyzing complex herbal extracts of Traditional Chinese Medicines.

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Fast microwave‐assisted extraction of rotenone for its quantification in seeds of yam bean (Pachyrhizus sp.)

Cited by 8 publications

References 17 publications

Degradation of Rotenone in Yam Bean Seeds (Pachyrhizus sp.) through Food Processing

Degradation of Rotenone in Yam Bean Seeds (Pachyrhizus sp.) through Food Processing

Advanced analytical techniques for the extraction and characterization of plant-derived essential oils by gas chromatography with mass spectrometry

Efficient optimization of ultra‐high‐ performance supercritical fluid chromatographic separation of Rosa sericea by response surface methodology

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