Fast human serum profiling through chemical depletion coupled to gold-nanoparticle-assisted protein separation

López-Cortés, Rubén; Oliveira, Elisabete; Núñez, Cristina; Lodeiro, Carlos; Cadena, Marı́a Páez de la; Fdez-Riverola, Florentino; López-Fernández, Hugo; Reboiro-Jato, Miguel; Glez-Peña, Daniel; Capelo, José Luís; Santos, Hugo M.

doi:10.1016/j.talanta.2012.08.020

Cited by 26 publications

(18 citation statements)

References 29 publications

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“…Serum profiling using GNPs previously done in our laboratory has addressed a number of parameters that need to be optimized, as follow: (i) GNPs/protein ratio, (ii) pH range and (iii) incubation time. In addition, we have shown in previous work [23] that proteins with and without cysteine amino acids, a.a, can be separated one from each other using gold nanoparticles. Proteins without cysteine a.a. remain in the supernatant and can be further investigated, if necessary, as we have also recently shown [49].…”

Section: Optimization Of Urine Profiling Using Gold Nanoparticlesmentioning

confidence: 91%

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A methodological approach based on gold-nanoparticles followed by matrix assisted laser desorption ionization time of flight mass spectrometry for the analysis of urine profiling of knee osteoarthritis

López-Cortés

Formigo

Reboiro-Jato

et al. 2016

Talanta

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The aim of this work is to develop a nanoparticle-based methodology to find out biomarkers of diagnostic for knee osteoarthritis, KOA, through the use of matrix assisted laser desorption ionization time-of-flight-based mass spectrometry profiling. Urine samples used for this study were obtained from KOA patients (42 patients), patients with prosthesis (58 patients), and controls (36 individuals) with no history of joint disease. Gold-nano particle MALDI-based urine profiling was optimized and then applied over the 136 individuals. Jaccard index and 10 different classifiers over MALDI MS datasets were used to find out potential biomarkers. Then, the specificity and sensitivity of the method were evaluated. The presence of ten m/z signals as potential biomarkers in the healthy versus non-healthy approach suggests that patients (KOA and prosthesis) are differentiable from the healthy volunteers through profiling. The automatic diagnostic study confirmed these preliminary conclusions. The sensitivity and the specificity for the urine profiling criteria here reported, achieved by the C4.5 classifier, is 97% and 69% respectively. Thus, it is confirmed the utility of the method proposed in this work as an additional fast, non-expensive and robust test for KOA diagnostic. When the proposed method is compared with those used in common practice it is found that sensitivity is the highest, thus with a low false negative rate for diagnostic KOA patients in the population studied. Specificity is lower but in the range accepted for diagnostic objectives.

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Section: Optimization Of Urine Profiling Using Gold Nanoparticlesmentioning

confidence: 91%

“…In previous studies we have described a high-throughput method for serum proteomic profiling in patients with multiple myeloma [23]. In the present work such method has been optimized and applied to the complexity of urine samples.…”

Section: Introductionmentioning

confidence: 99%

A methodological approach based on gold-nanoparticles followed by matrix assisted laser desorption ionization time of flight mass spectrometry for the analysis of urine profiling of knee osteoarthritis

López-Cortés

Formigo

Reboiro-Jato

et al. 2016

Talanta

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“…An internal standard allows product quantification, using a-cyano-4-hydroxicinnamic acid (CHCA) as an organic matrix for MALDI-TOF. Following a similar principle, López-Cortes et al [13] have utilized AuNPs to separate serum proteins for subsequent fingerprinting identification by MALDI-TOF-MS. A mixture of 14 nm and 3.5 nm citrate-capped AuNPs was used for separation and analysis of biomolecules containing thiol groups, as the thiol groups show high affinity toward the gold surface and binding is strong and extremely likely to occur [14]. Aptamer-gold nanoparticle conjugates have also been used as affinity probes for SALDI-TOF analysis of adenosine triphosphate (ATP) and glutathione (GSH), improving sensitivity when compared to traditional matrices [15] -see Fig.…”

Section: Unmodified and Modified Nanoparticles For Sample Preparationmentioning

confidence: 99%

“…Other NPs, such as graphitized carbon NPs, have been used for SALDI-TOF/TOF-MS quantification of citrulline [73] via a novel method of tandem MS-parallel fragmentation monitoring (PFM) using graphitized carbon NPs as a surface for LDI. This way, it was possible to quantify citrulline and an isotopic analog that is 1 mass unit Handbook of Nanoparticles DOI 10.1007/978-3-319-13188-7_32-1 # Springer International Publishing Switzerland 2015 heavier ([ureido- 13 C] citrulline). Another approach used graphene-coated cobalt NPs functionalized with benzylamine groups for target enrichment and subsequent direct SALDI analysis of organic compounds [85].…”

Section: Carbon-containing Nanoparticlesmentioning

confidence: 99%

Nanoparticles for Mass Spectrometry Applications

Larguinho

Capelo

Baptista

2015

Handbook of Nanoparticles

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Nanotechnology has led to the development of new and improved materials, and particular emphasis has been directed toward nanoparticles and their multiple bio-applications. Nanoparticles exhibit size-, shape-, and composition-dependent properties, e.g., surface plasmon resonance and photothermal properties, which may potentially enhance laser desorption/ionization systems for mass spectrometry-based analysis of biomolecules. Also, nanoparticles possess high surface to volume ratio that can be easily derivatized with a wide range of ligands with different functional groups. Surface modification makes nanoparticles advantageous for sample preparation procedures prior to detection by mass spectrometry. Moreover, it allows the synthesis of affinity probes, which promotes interactions between nanoparticles and analytes, greatly enhancing the ionization efficiency.This chapter provides a comprehensive discussion on the use of nanoparticles for mass spectrometryrelated applications, from sample preparation methodologies to ionization surfaces. Applications will focus on nanoparticle size, composition, and functionalization, as a comparative point of view on optimal characteristics toward maximization of bioassay efficiency.

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“…The functionality of MALDIViz application is demonstrated by analysis of in-house-generated screening data from an investigation of different solid-phase extraction (SPE) materials 17 and a publicly available cancer dataset. 18 The MALDIViz application is accessible through https://jkkishore85.shinyapps.io/maldiviz/.…”

Section: Introductionmentioning

confidence: 99%

MALDIViz: A Comprehensive Informatics Tool for MALDI-MS Data Visualization and Analysis

Jagadeesan

Ekström

2017

SLAS Discovery

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Recently, mass spectrometry (MS) has emerged as an important tool for high-throughput screening (HTS) providing a direct and label-free detection method, complementing traditional fluorescent and colorimetric methodologies. Among the various MS techniques used for HTS, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides many of the characteristics required for high-throughput analyses, such as low cost, speed, and automation. However, visualization and analysis of the large datasets generated by HTS MALDI-MS can pose significant challenges, especially for multiparametric experiments. The datasets can be generated fast, and the complexity of the experimental data (e.g., screening many different sorbent phases, the sorbent mass, and the load, wash, and elution conditions) makes manual data analysis difficult. To address these challenges, a comprehensive informatics tool called MALDIViz was developed. This tool is an R-Shiny-based web application, accessible independently of the operating system and without the need to install any program locally. It has been designed to facilitate easy analysis and visualization of MALDI-MS datasets, comparison of multiplex experiments, and export of the analysis results to high-quality images.

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Fast human serum profiling through chemical depletion coupled to gold-nanoparticle-assisted protein separation

Cited by 26 publications

References 29 publications

A methodological approach based on gold-nanoparticles followed by matrix assisted laser desorption ionization time of flight mass spectrometry for the analysis of urine profiling of knee osteoarthritis

A methodological approach based on gold-nanoparticles followed by matrix assisted laser desorption ionization time of flight mass spectrometry for the analysis of urine profiling of knee osteoarthritis

Nanoparticles for Mass Spectrometry Applications

MALDIViz: A Comprehensive Informatics Tool for MALDI-MS Data Visualization and Analysis

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