Fast, high‐resolution in vivo cine magnetic resonance imaging in normal and failing mouse hearts on a vertical 11.7 T system

Schneider, Jürgen; Cassidy, P J; Lygate, Craig A.; Tyler, Damian J.; Wiesmann, Frank; Grieve, Stuart M.; Hulbert, Karen; Clarke, Kieran; Neubauer, Stefan

doi:10.1002/jmri.10411

Cited by 133 publications

(160 citation statements)

References 23 publications

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“…Using the standard analysis as gold standard, the 3D GPM method provided similar results in less than a fifth of the analysis time. Interobserver reproducibility using standard analysis has previously been reported at 11.7 T to be 5 Ϯ 3% in LV mass, 3 Ϯ 3% in EDV, and 5 Ϯ 6% in ESV (10). In the current study we measured the scan-rescan reproducibility rather than interobserver variability, since this is known to be a more stringent test of the reproducibility of the measurement (14) including differences in positioning, heart rate, and cardiac loading as commonly observed in repeated measures experimental designs.…”

Section: Discussionmentioning

confidence: 96%

“…The standard manual analysis of mouse cardiac MRI examinations has been previously shown to provide accurate estimates of LV mass compared with autopsy measurements (10). Using the standard analysis as gold standard, the 3D GPM method provided similar results in less than a fifth of the analysis time.…”

Section: Discussionmentioning

confidence: 96%

“…C57Bl6/J mice (n ϭ 6, body weight 26.2 Ϯ 1.1 g, male) were anesthetized with isoflurane (1.0%-1.5% in oxygen, 1.5 L/min). Animals were positioned prone on a dedicated mouse cradle and monitored for heart rate and respiration using needle-electrodes inserted subcutaneously into the forelimbs to derive the ECG, and a pressure-pad (rather than a conductor loop) to monitor respiration (10). Temperature was maintained as described previously (10).…”

Section: Imaging Experimentsmentioning

confidence: 99%

“…After scouting, manual tuning and matching the coil, and slice selective shimming, ECG and respiratory gated 2D spoiled gradient echo cine images were acquired in the short axis orientation in 8 -9 contiguous slices, 1 mm thick, covering the LV as described previously (10). The imaging parameters were: TE/TR ϭ 1.8/ 4.6 msec; field-of-view (FOV) ϭ 25.6 ϫ 25.6 mm, bandwidth ϭ 147 kHz.…”

Section: Imaging Experimentsmentioning

confidence: 99%

“…For each dataset, LVM and EDV and ESV volumes were determined by a single operator, blinded to animal ID, date of study, and acquisition scheme, using Amira 4.1.2 (TGS/Mercury Systems, Merignac Cedex, France) as described previously (10). The number of voxels of each compartment multiplied by the voxel size yielded the respective volumes.…”

Section: Standard Analysismentioning

confidence: 99%

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Fast left ventricular mass and volume assessment in mice with three‐dimensional guide‐point modeling

Young

Barnes

Davison

et al. 2009

Magnetic Resonance Imaging

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Purpose: To investigate the accuracy (vs. standard manual analysis) and precision (scan-rescan reproducibility) of three-dimensional guide-point modeling (GPM) for the assessment of left ventricular (LV) function in mice. Methods:Six male wildtype C57/Bl6 mice (weight 26.2 Ϯ 1.1 g) were scanned twice, 3 days apart. Each scan was performed twice, at 0.2 mm/pixel with one average and at 0.1 mm/pixel with two averages. The 24 studies were anonymized and analyzed in blinded fashion using GPM and standard manual slice summation. Results:The average error between GPM and standard analysis was 2.3 Ϯ 5.8 mg in mass, 1.7 Ϯ 3.2 L in enddiastolic volume, 2.3 Ϯ 3.1 L in end-systolic volume, Ϫ2.7 Ϯ 4.3% in ejection fraction, Ϫ0.6 Ϯ 3.3 L in stroke volume, and Ϫ0.31 Ϯ 1.56 ml ⅐ min Ϫ1 in cardiac output (mean difference Ϯ SD of differences, n ϭ 24). The average time taken was 8.0 Ϯ 2.5 minutes for 3D GPM and 48.5 Ϯ 8.9 minutes for standard analysis (n ϭ 24). Scan-rescan reproducibility results were similar to the standard analysis. No significant differences were found using linear mixed effects modeling in either accuracy or precision between scan resolutions or analysis method. Conclusion:3D GPM enables fast analysis of mouse LV function, with similar accuracy and reproducibility to standard analysis. An image resolution of 0.2 mm/pixel with one average is adequate for LV function studies.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 96%

Section: Imaging Experimentsmentioning

confidence: 99%

Section: Imaging Experimentsmentioning

confidence: 99%

Section: Standard Analysismentioning

confidence: 99%

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Fast left ventricular mass and volume assessment in mice with three‐dimensional guide‐point modeling

Young

Barnes

Davison

et al. 2009

Magnetic Resonance Imaging

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Cardiovascular Disorders: Insights into In Vivo Cardiovascular Phenotyping

Monassier¹,

Constantinesco²

2006

Standards of Mouse Model Phenotyping

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Intercomparison of performance of RF coil geometries for high field mouse cardiac MRI

Constantinides¹,

Angeli²,

Gkagkarellis³

et al. 2011

Concepts Magnetic Resonance

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Multi-turn spiral surface coils are constructed in flat and cylindrical arrangements and used for high field (7.1 T) mouse cardiac MRI. Their electrical and imaging performances, based on experimental measurements, simulations, and MRI experiments in free space, and under phantom, and animal loading conditions, are compared with a commercially available birdcage coil. Results show that the four-turn cylindrical spiral coil exhibits improved relative SNR (rSNR) performance to the flat coil counterpart, and compares fairly well with a commercially available birdcage coil. Phantom experiments indicate a 50% improvement in the SNR for penetration depths ≤ 6.1 mm from the coil surface compared to the birdcage coil, and an increased penetration depth at the half-maximum field response of 8 mm in the 4-spiral cylindrical coil case, in contrast to 2.9 mm in the flat 4-turn spiral case. Quantitative comparison of the performance of the two spiral coil geometries in anterior, lateral, inferior, and septal regions of the murine heart yield maximum mean percentage rSNR increases of the order of 27–167% in vivo post-mortem (cylindrical compared to flat coil). The commercially available birdcage outperforms the cylindrical spiral coil in rSNR by a factor of 3–5 times. The comprehensive approach and methodology adopted to accurately design, simulate, implement, and test radiofrequency coils of any geometry and type, under any loading conditions, can be generalized for any application of high field mouse cardiac MRI.

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Fast, high‐resolution in vivo cine magnetic resonance imaging in normal and failing mouse hearts on a vertical 11.7 T system

Cited by 133 publications

References 23 publications

Fast left ventricular mass and volume assessment in mice with three‐dimensional guide‐point modeling

Fast left ventricular mass and volume assessment in mice with three‐dimensional guide‐point modeling

Cardiovascular Disorders: Insights into In Vivo Cardiovascular Phenotyping

Intercomparison of performance of RF coil geometries for high field mouse cardiac MRI

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