Cardiovascular Disorders: Insights into
            <i>In Vivo</i>
            Cardiovascular Phenotyping

Monassier, Laurent; Constantinesco, A.

doi:10.1002/9783527611942.ch7

Cited by 5 publications

(5 citation statements)

References 61 publications

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“…Acute myocardial infarction has been mimicked in a variety of rat and mouse models. Among these, the murine model of myocardial infarction (MMMI) has been extensively studied as a means of clarifying the functional, structural and molecular changes associated with clinical ischemic heart disease, and testing the effectiveness of new pharmacological therapies [2]. In this context, the precise evaluation of the degree and extent of myocardial injury after an acute ischemic event is needed to interpret correctly the physiological results.…”

Section: Introductionmentioning

confidence: 99%

Feasibility of quantitative analysis of regional left ventricular function in the post-infarct mouse by magnetic resonance imaging with retrospective gating

Franzosi

Guerrini

Castiglioni

et al. 2011

Computers in Biology and Medicine

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We aimed testing feasibility of identification of regional left ventricular (LV) endocardial motion abnormalities in mice undergoing coronary ligation (MI), using cine magnetic resonance with retrospective gating and computation of regional fractional area change (RFAC), by comparison with histological "gold standard" evaluation. ROC analysis determined the optimal RFAC cut-off values for detecting regional ischemic injury. This approach was tested on 18 MI and 10 sham mice. Automated regional LV motion interpretation and bull's eye display allowed non-invasive localization of the induced infarction. Possible applications to future studies assessing the effectiveness of pharmacological treatments or regenerative medicine are expected.

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Section: Introductionmentioning

confidence: 99%

Feasibility of quantitative analysis of regional left ventricular function in the post-infarct mouse by magnetic resonance imaging with retrospective gating

Franzosi

Guerrini

Castiglioni

et al. 2011

Computers in Biology and Medicine

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“…Finally, it is a high-cost technique along with low throughput which in turn restricts its use. These drawbacks tend currently to push MRI toward being a useful tool for second-line specialized investigations in small animal phenotype imaging (Monassier and Constantinesco 2006). Given these facts and considering the small amount of work done lowering the main magnetic field B 0 , it is interesting to evaluate the performance of a low magnetic field (0.1 T) for in vivo small animal imaging and not to take for definitive the initial results obtained on small animals many years ago using low magnetic fields.…”

Section: Introductionmentioning

confidence: 99%

Dedicated low-field MRI in mice

et al. 2009

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The rationale of this work is to point out the relevance of in vivo MR images of mice obtained using a dedicated low-field system. For this purpose a small 0.1 T water-cooled electro-magnet and solenoidal radio frequency (RF) transmit-receive coils were used. All MR images were acquired in three-dimensional (3D) mode. An isolation cell was designed allowing easy placement of the RF coils and simple delivery of gaseous anesthesia as well as warming of the animal. Images with and without contrast agent were obtained in total acquisition times on the order of half an hour to four hours on normal mice as well as on animals bearing tumors. Typical in plane pixel dimensions range from 200 x 200 to 500 x 500 microm(2) with slice thicknesses ranging between 0.65 and 1.50 mm. This work shows that, besides light installation and low cost, dedicated low-field MR systems are suitable for small rodents imaging, opening this technique even to small research units.

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“…Non-invasive preclinical imaging opens new possibilities for long-term follow-up in mice models of cardiovascular diseases. The transfer to small animal of human medical imaging techniques as ultrasound, magnetic resonance imaging (MRI), X-ray-computed tomography (micro-CT), positron emission tomography (micro-PET), planar scintigraphy and single-photon emission computed tomography (micro-SPECT) offers new possibilities for in vivo mouse quantification of ventricular function and cardiac metabolisms [1][2][3][4]. Transthoracic echography using a high-frequency probe allows us to obtain at a low cost images of selected 2D slices of the left ventricle (LV) in real time with high spatial and good temporal resolutions with pixel size of 0.1 × 0.1 mm 2 and slice thickness of 1 mm at 15 MHz, while acquiring 9-20 images per cardiac cycle, depending on the acquisition conditions [5].…”

Section: Introductionmentioning

confidence: 99%

Assessment of right and left ventricular function in healthy mice by blood-pool pinhole gated SPECT

Goetz

Monassier

Choquet

et al. 2008

Comptes Rendus. Biologies

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The feasibility of blood-pool pinhole ECG gated SPECT was investigated in healthy mice to assess right and left ventricular function analysis. Anaesthetized (isoflurane 1-1.5%) adult CD1 mice (n = 11) were analyzed after intravenous administration of 0.2 ml of 550 MBq of 99m Tc human albumin. For blood-pool gated SPECT imaging, 48 ventral step and shoot projections with eight time bins per RR over 180 • with 64 × 64 word images were acquired with a small animal gamma camera equipped with a pinhole collimator of 12 cm in focal length and 1.5 mm in diameter. For appropriate segmentation of right and left ventricular volumes, a 4D Fourier analysis was performed after reconstruction and reorientation of blood-pool images with a voxel size of 0.55 × 0.55 × 0.55 mm 3 . Average right and left ejection fractions were respectively 52 ± 4.7% and 65 ± 5.2%. Right end diastolic and end systolic volumes were significantly higher compared with the corresponding left ventricular volumes (P < 0.0001 each). A linear correlation between right and left stroke volumes (r = 0.9, P < 0.0001) was obtained and right and left cardiac outputs were not significantly different 14.2 ± 1.9 and 14.1 ± 2 ml/min, respectively. To cite this article: C.

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Cardiovascular Disorders: Insights into In Vivo Cardiovascular Phenotyping

Cited by 5 publications

References 61 publications

Feasibility of quantitative analysis of regional left ventricular function in the post-infarct mouse by magnetic resonance imaging with retrospective gating

Feasibility of quantitative analysis of regional left ventricular function in the post-infarct mouse by magnetic resonance imaging with retrospective gating

Dedicated low-field MRI in mice

Assessment of right and left ventricular function in healthy mice by blood-pool pinhole gated SPECT

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