Assessment of right and left ventricular function in healthy mice by blood-pool pinhole gated SPECT

Abstract

The feasibility of blood-pool pinhole ECG gated SPECT was investigated in healthy mice to assess right and left ventricular function analysis. Anaesthetized (isoflurane 1-1.5%) adult CD1 mice (n = 11) were analyzed after intravenous administration of 0.2 ml of 550 MBq of 99m Tc human albumin. For blood-pool gated SPECT imaging, 48 ventral step and shoot projections with eight time bins per RR over 180 • with 64 × 64 word images were acquired with a small animal gamma camera equipped with a pinhole collimator of 12 cm in focal length and 1.5 mm in diameter. For appropriate segmentation of right and left ventricular volumes, a 4D Fourier analysis was performed after reconstruction and reorientation of blood-pool images with a voxel size of 0.55 × 0.55 × 0.55 mm 3 . Average right and left ejection fractions were respectively 52 ± 4.7% and 65 ± 5.2%. Right end diastolic and end systolic volumes were significantly higher compared with the corresponding left ventricular volumes (P < 0.0001 each). A linear correlation between right and left stroke volumes (r = 0.9, P < 0.0001) was obtained and right and left cardiac outputs were not significantly different 14.2 ± 1.9 and 14.1 ± 2 ml/min, respectively. To cite this article: C.

Single-cell transcriptomic analysis identifies murine heart molecular features at embryonic and neonatal stages

“…Third, as described before, we profiled the CD1 hearts from E18.5 and P1. Considering that the CD1 strain has a low penetrance of developing cardiac phenotypes and has been mostly used to study normal heart developmental processes 27 , 48 , 49 , we also profiled heart cells in C57BL/6—a mouse strain frequently used to model heart defects 50 , 51 . We analyzed C57BL/6 mouse hearts from E9.5 to P9.…”

PET, SPECT, CT, and MRI in Mouse Cardiac Phenotyping: An Overview

“…99m Tclabeled red blood cells were used for blood pool imaging in ECG gated micro-SPECT, demonstrating that murine LV volume assessment is possible with high voxel and temporal resolutions (Chin et al, 2007;Goetz et al, 2008a). ECG-gated blood pool micro-SPECT measurement of right ventricular volumes was also recently demonstrated in healthy living mice (Goetz et al, 2008b).…”

“…• Assessment of LV size, shape, mass, global mechanical function (ejection fraction, end‐systolic and end‐diastolic volumes, stroke volume, cardiac output), and wall thickening using 99m Tc‐tetrofosmin, 99m Tc‐sestamibi, 201 Tl, and 99m Tc‐red blood cells. Assessment of global mechanical function of the right ventricle (ejection fraction, end‐systolic and end‐diastolic volumes, stroke volume, cardiac output) using 99m Tc‐red blood cells (Wu et al, ; Constantinesco et al, ; Chin et al, ; Goetz et al, ,b).…”

Influence of Respiratory Gating, Image Filtering, and Animal Positioning on High-Resolution Electrocardiography-Gated Murine Cardiac Single-Photon Emission Computed Tomography

“…10,11 ECG gating has been evaluated for small animal SPECT for assessing left ventricular function and has been applied in studies where new pharmaceuticals were tested. [12][13][14][15][16][17][18] Simultaneous ECG and respiratory gating has been assessed for a high-resolution (1 mm) microPET system. 19 In this study, it was found that although respiratory motion was detectable in the images, its spatial extent and duration were small, and it could therefore likely be ignored for most studies.…”