Fast gel‐permeation chromatography. I. A study of operational parameters

Abstract

SynopsisA systematic study of factors affecting the GPC separation showed that peak spreading with increasing flow rate was much less than predicted from the Van Deemter equation. Viscous fingering decreased and peak symmetry improved a t increased flow rates. As a result, fast GPC analysis was shown to be readily attainable through optimization of operating parameters.

Retention

“…The fact that this occurs in SEC columns is supported by two sets of studies, one showing that SEC retention is independent of flow rate [10,11] and the other providing measurement of equilibrium solute distribution through a series of simple static mixing experiments [12,13]. The fact that this occurs in SEC columns is supported by two sets of studies, one showing that SEC retention is independent of flow rate [10,11] and the other providing measurement of equilibrium solute distribution through a series of simple static mixing experiments [12,13].…”

Special Techniques

“…High-speed SEC has been a subject of interest for nearly as long as SEC itself has existed (see Figure 2.5) [25,26]. High-speed SEC has been a subject of interest for nearly as long as SEC itself has existed (see Figure 2.5) [25,26].…”

Experimental Variables and Techniques

“…Retention volumes of macromolecules injected at low concentrations in small volumes are essentially independent of flow rate for practical SEC systems, as shown in Figures 2.4 and 2.5 [16,17]. In Figure 2.5, flow rates of 0.1 to 12.5 mL/min were used with 0.85-cm-i.d.…”