Fast‐GC/HRMS to quantify the EU priority PAH

Abstract

For the analysis of the 15 polycyclic aromatic hydrocarbons (PAH) classified as priority from the Scientific Committee on Food (SCF) and benzo[c]fluorene (BcL) assessed to be relevant by the Joint FAO/WHO Experts Committee on Food Additives (JECFA) in food, a sensitive analytical method is necessary. In the past, many methods were established for only the analysis of the 16 priority PAH recommended by the US Environmental Protection Agency (16 EPA-PAH) but not for the 15 SCF-PAH and BcL. Therefore, at the Max-Rubner-Institut, Federal Research Institute of Nutrition and Food in Kulmbach, Germany, an analytical method with a runtime of 72 min was developed. To shorten this runtime without a loss of separation, a Fast-GC/high resolution MS (HRMS) method with a runtime of only 25 min using a TR-50ms column (10 m x 0.1 mm x 0.1 microm) was created. The repeatability (n = 3) of spiked PAH concentrations in test-matrix tea ranged from 0.1 to 11%. The analytical parameters LOD (0.01-0.02 microg/kg) and LOQ (0.03-0.06 microg/kg) also in the test-matrix tea were determined. A good linearity for all PAH (R(2) >0.99) and averaged recoveries from 75 to 117% in the concentration range of 1-20 microg/kg were achieved.

GC-MS Technique and its Analytical Applications in Science and Technology

“…It is widely used in pure and applied sciences like Chemistry, Polymers, Nanotechnology and Biotechnology etc. It yields useful information that can be used in research publication internationally [21,22]. …”

Organic Micropollutants

“…Using a liquid-crystal stationary phase in combination with GC-MS, complete separation of the USEPA 16 priority parent PAHs was achieved in less than 5 min on a 6 m × 0.1 mm ID column, and in less than 7 min on a 15 m × 0.25 mm ID column (Britten and Naikwadi, 2008). Fast GC-HRMS was used to separate 16 PAH compounds of 216-302 Da, from benzo[c]fluorene to dibenzo[a, h]pyrene (Ziegenhals et al ., 2008). These compounds represent priority PAHs within the EU as defined by the Scientific Committee on Food (SCF-PAH), which could include shellfish.…”

Searching ingredients polluted by polycyclic aromatic hydrocarbons in feeds due to atmospheric or pyrolytic sources

“…In this way, the inherent disadvantages of traditional LLE techniques, such as the large volumes of organic solvents, time-consuming and the multi-step processes required, that always risk the loss of some analytes, are settled (Aamot, Steinnes, & Schmid, 1996). Several methods have been reported for detection and determination of the selected PAHs with gas chromatography coupled with mass spectrometry (GC-MS) (Veyrand et al, 2007), fast-GC high resolution MS (Fast-GC-HRMS) (Ziegenhals, Hubschmann, Speer, & Jira, 2008), in various matrices. Compared to GC-MS, LC-FD presents numerous advantages when applied to the study of PAHs in food samples: a complex cleanup is unnecessary, reducing the likely sources of error and increasing absolute recoveries; and there is no need to use ''appropriate'' internal standards to correct for poor recovery and/or instrumental errors due to injection discrimination (Rey-Salgueiro et al, 2008a).…”