Fast extraction, amplification and analysis of genes from human blood

Zhang, Lihua; Dang, Fuquan; Kaji, Noritada; Baba, Yoshinobu

doi:10.1016/j.chroma.2005.10.071

Cited by 11 publications

(5 citation statements)

References 33 publications

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“…Recently, this commercial CE chip has been used for off-line detection of PCR products (10,11,13,16,69,73). Other microchip electrophoresis systems include the Hitachi SV 12-channel electrophoresis microchip (Hitachi Electronics Co.) (73), LabChip Õ 90 Automated Electrophoresis System (Caliper Lifesciences) (20) and CE chips with laserinduced fluorescence (LIF) detection (41,52,53,56). The off-line CE chip platform can offer walkaway and unattended analysis of DNA molecules, eliminate the time-consuming and messy slab gel process, generate much more reproducible and high quality data, and allow high-throughput laboratory analysis; however, the manual sample loading may increase the risk of cross contamination and the total analysis time.…”

Section: Off-line Detection Methodsmentioning

confidence: 99%

“…The static treatment involves the pre-coating of chip surface, during fabrication of PCR chip or immediately before use, with one of the following substances: SiO 2 (10,14,20,21,25), bovine serum albumin (BSA) (11,17,25,37,53,61,73), polyethylene glycol (PEG) (65,78,82) or silanizing agents (for example 3-glycidoxypropyl trimethoxysilane (11), dichlorodimethylsilane (23,69), Sigmacoat Õ (16,29) or trimethylchlorosilane (33). The SiO 2 pre-coating is a reproducible and inexpensive standard MEMS process and can be accomplished in a batch fashion.…”

Section: Surface Treatmentmentioning

confidence: 99%

“…off-line and on-line detection, are covered, followed by integration of functional components in PCR chips, biological samples used in PCR chips and potential applications of PCR chips, as well as practical issues related to the development of PCR chips. (69), cyclic olefin copolymer (COC) (65), Gene Frame Õ (70), perfluoroalkoxy-modified polytetrafluoroethylene (PFA) (13,(71)(72)(73)(74)(75)(76), LiNbO 3 (77) and 317 stainless steel (78), have also been used in PCR microfluidic devices. Each substrate has different properties and therefore different advantages and disadvantages.…”

Section: Introductionmentioning

confidence: 99%

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Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Zhang

Xing

2007

Nucleic Acids Research

379

289

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The possibility of performing fast and small-volume nucleic acid amplification and analysis on a single chip has attracted great interest. Devices based on this idea, referred to as micro total analysis, microfluidic analysis, or simply ‘Lab on a chip’ systems, have witnessed steady advances over the last several years. Here, we summarize recent research on chip substrates, surface treatments, PCR reaction volume and speed, architecture, approaches to eliminating cross-contamination and control and measurement of temperature and liquid flow. We also discuss product-detection methods, integration of functional components, biological samples used in PCR chips, potential applications and other practical issues related to implementation of lab-on-a-chip technologies.

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Section: Off-line Detection Methodsmentioning

confidence: 99%

Section: Surface Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Zhang

Xing

2007

Nucleic Acids Research

379

289

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“…In the years since its development, microchip capillary electrophoresis (CE) has established itself as an important research area in chemical and biochemical analysis. Many different applications have been demonstrated using this chemistry, including DNA sequencing and fragment analysis, − proteomic separations, , and environmental analysis , to name a few. The great interest in microchip CE and microfluidics in general is due to the potential to generate complex chemical synthesizers and analyzers that provide the power of an entire chemical laboratory on a single chip .…”

mentioning

confidence: 99%

Generation of Hydrophilic Poly(dimethylsiloxane) for High-Performance Microchip Electrophoresis

2006

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Poly(dimethylsiloxane) (PDMS) has become one of the most widely used materials for microchip capillary electrophoresis and microfluidics. The popularity of this material is the result of its low cost, simple fabrication, and rugged elastomeric properties. The hydrophobic nature of PDMS, however, limits its applicability for microchip CE, microfluidic patterning, and other nonelectrophoresis applications. The surface of PDMS can be made hydrophilic using a simple air plasma treatment; however, this property is quickly lost through hydrophobic recovery caused by diffusion of unreacted oligomer to the surface. Here, a simple approach for the generation of hydrophilic PDMS with long-term stability in air is presented. PDMS is rendered hydrophilic through a simple two-step extraction/oxidation process. First, PDMS is extracted in a series of solvents designed to remove unreacted oligomers from the bulk phase. Second, the oligomer-free PDMS is oxidized in a simple air plasma, generating a stable layer of hydrophilic SiO2. The conversion of surface-bound siloxane to SiO2 was followed with X-ray photoelectron spectroscopy. SiO2 on extracted-oxidized PDMS was stable for 7 days in air as compared to less than 3 h for native PDMS. Furthermore, the contact angle for modified PDMS was reduced to <40 degrees and remained low throughout the experiments. As a result of the decreased contact angle, capillary channels self-wet through capillary action, making the microchannels much easier to fill. Finally, the modification significantly improved the performance of the devices for microchip electrophoresis. The electroosmotic flow increased from 4.1 x 10(-4) to 6.8 x 10(-4) cm(2)/V.s for native compared to oxidized PDMS. Separation efficiencies for electrochemical detection also increased from 50 000 to 400 000 N/m for a 1.1-nL injection volume. The result of this modification is a significant improvement in the performance of PDMS for microchip electrophoresis and microfluidic applications.

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“…Μια άλλη ευρέως χρησιμοποιούμενη τεχνική είναι η τριχοειδής ηλεκτροφόρηση (CE) (Jorgenson JW, and Lukasc KD, 1983 (Poulsen CR, et al 2005;Hataoka Y, et al 20005;Lee JG, et al 2006). Άλλες CE ψηφίδες είναι η Hitachi 12-channel (Hitachi Electronics) (Zhang LH, et al 2006), LabChip" (Caliper Lifesciences) (Oh KW, et al 2006).…”

Section: μέθοδοι ανίχνευσηςunclassified

Η Μικρο/Νανοτεχνολογία Στην Ανάλυση DNA

Tataridis¹,

Ταταρίδης²

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Η παρούσα διδακτορική διατριβή πραγματεύεται την αξιοποίηση της μικρο- και νανοτεχνολογίας στην ανάλυση του DNA. Περιγράφεται η ανάπτυξη φθορισμομετρικής μεθόδου εκτίμησης της ποιότητας μικροσυστατικών δίκλωνου DNA, δηλαδή της αποτελεσματικής της εναπόθεσης του DNA, της μορφολογίας των κηλίδων, της λειτουργικότητας των μορίων DNA που έχουν αποτεθεί και της επαναληψιμότητας της εναπόθεσης. Η μέθοδος επίσης παρέχει τη δυνατότητα εντοπισμού θέσεων όπου δεν έχει αποτεθεί DNA. Πραγματοποιήθηκαν μελέτες για τη βελτιστοποίηση της απόδοσης της αλυσιδωτής αντίδρασης πολυμεράσης σε μικρορρευστονική ψηφίδα συνεχούς ροής. Τέλος, αναπτύχθηκε μέθοδος ανίχνευσης μονονουκλεοτιδικών πολυμορφισμών στο γενωμικό DNA, η οποία συνδυάζει τα πλεονεκτήματα ψηφίδας για την εκτέλεση PCR συνεχούς ροής και βιοαισθητήρα DNA τύπου ξηρών αντιδραστηρίων για την ταχεία ανίχνευση των προϊόντων της PCR χωρίς τη χρήση οργάνων. Η μέθοδος εφαρμόστηκε στην ανίχνευση μετάλλαξης στο γονίδιο της β-σφαιρίνης.

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Fast extraction, amplification and analysis of genes from human blood

Cited by 11 publications

References 33 publications

Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Generation of Hydrophilic Poly(dimethylsiloxane) for High-Performance Microchip Electrophoresis

Η Μικρο/Νανοτεχνολογία Στην Ανάλυση DNA

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