Fast excitatory postsynaptic potentials and the responses to excitant amino acids of sympathetic preganglionic neurons in the slice of the cat spinal cord

Inokuchi, Hiroe; Yoshimura, Michihiro; Yamada, Satoshi; Polosa, C.; Nishi, S.

doi:10.1016/0306-4522(92)90152-r

Cited by 50 publications

(16 citation statements)

References 22 publications

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“…The voltage sensitivity was relieved by removal of magnesium from the bathing medium. These observations are consistent with the voltagedependent block of NMDA receptors by magnesium described previously in SPNs (Shen et al 1990;Spanswick & Logan, 1990;Inokuchi et al 1992;Krupp & Feltz, 1995), and on other central neurones (Mayer, Westbrook & Guthrie, 1984;Nowak, Bregestovski, Ascher, Herbert & Prochiantz, 1984). Pharmacological isolation of this NMDA receptormediated component of synaptic transmission elicited from the iLF, cLF or cIML revealed an EPSP or EPSC in normal magnesium-containing bathing medium that showed a constant latency and rise time and no failures in response to high-frequency stimulation, and could reach threshold for firing, conclusive evidence of a monosynaptic role for NMDA receptors in SPNs.…”

Section: Origins Of Ipsi-and Contralaterally Evoked Monosynaptic Epspssupporting

confidence: 79%

“…These antagonists suppressed the early fast EPSP and EPSCs in all neurones, with NBQX being the most, and GYKI 52466 the least effective of these antagonists. That non-NMDA (AMPAÏkainate) receptors mediate a fast synaptic response when elicited from the iLF is in agreement with previous studies (Shen et al 1990;Inokuchi et al 1992;Krupp & Feltz, 1995;Sah & McLachlan, 1995). However, the relative contribution of NMDA receptors to synaptic transmission elicited from the iLF has remained somewhat unclear.…”

Section: Origins Of Ipsi-and Contralaterally Evoked Monosynaptic Epspssupporting

confidence: 78%

“…An investigation in neonatal rat transverse spinal cord slices suggested an exclusive role for non-NMDA receptors in pathways projecting from the iLF and NMDA receptors exclusive to pathways elicited from the DH (Shen et al 1990). However, data presented here, and by others in the neonatal rat (Krupp & Feltz, 1995) and adult cat (Inokuchi et al 1992), suggest a dual role for both NMDA and non-NMDA receptors. Similarly in early neonatal animals, dual-component EPSPs were evoked in SPNs by stimulation of the ventrolateral medulla (Deuchars et al 1995).…”

Section: Origins Of Ipsi-and Contralaterally Evoked Monosynaptic Epspsmentioning

confidence: 58%

“…However, data from a recent study suggest that both NMDA and non-NMDA receptors mediate synaptic transmission in segmental and descending pathways (Krupp & Feltz, 1995). Similar dual-component NMDA and non-NMDA receptor-mediated EPSPs have been observed in adult cat (Inokuchi et al 1992), and recent studies in neonatal rats indicate a role for NMDA and non-NMDA receptors in pathways from the ventrolateral medulla (Deuchars, Morrison & Gilbey, 1995). We have utilized a spinal cord slice preparation to investigate the role of EAA receptors in mediating fast excitatory synaptic transmission to SPNs.…”

mentioning

confidence: 80%

“…EAAs excite SPNs when applied iontophoretically in vivo (Backman & Henry, 1983) and selective EAA-receptor agonists have been shown to excite SPNs in vitro (Shen, Mo & Dun, 1990;Spanswick & Logan, 1990;Inokuchi, Yoshimura, Yamada, Polosa & Nishi, 1992). The intermediolateral nucleus (IML), where the majority of SPNs are located, contains binding sites for the EAAreceptor agonist kainate (Morrison, Ernsberger, Milner, Callaway, Gong & Reis, 1989) and glutamate-immunoreactive synapses have been shown to impinge on SPNs Llewellyn-Smith, Phend, Minson, Pilowsky & Chalmers, 1992) as have synapses immunoreactive for phosphate-activated glutaminase, which catalyses the conversion of glutamine to glutamate (Chiba & Kaneko, 1993).…”

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confidence: 99%

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Bilaterally evoked monosynaptic EPSPs, NMDA receptors and potentiation in rat sympathetic preganglionic neurones in vitro

Spanswick

Renaud

Logan

1998

The Journal of Physiology

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Whole‐cell patch clamp and intracellular recordings were obtained from 190 sympathetic preganglionic neurones (SPNs) in spinal cord slices of neonatal rats. Fifty‐two of these SPNs were identified histologically as innervating the superior cervical ganglion (SCG) by the presence of Lucifer Yellow introduced from the patch pipette and the appearance of retrograde labelling following the injection of rhodamine‐dextran‐lysine into the SCG. Electrical stimulation of the ipsilateral (n= 71) or contralateral (n= 32) lateral funiculi (iLF and cLF, respectively), contralateral intermediolateral nucleus (cIML, n= 41) or ipsilateral dorsal horn (DH, n= 34) evoked EPSPs or EPSCs that showed a constant latency and rise time, graded response to increased stimulus intensity, and no failures, suggesting a monosynaptic origin. In all neurones tested (n= 60), fast rising and decaying components of EPSPs or EPSCs evoked from the iLF, cLF, cIML and DH in response to low‐frequency stimulation (0.03‐0.1 Hz) were sensitive to non‐NMDA receptor antagonists. In approximately 50 % of neurones tested (n= 29 of 60), EPSPs and EPSCs evoked from the iLF, cLF, cIML and DH during low‐frequency stimulation were reduced by NMDA receptor antagonists. In the remaining neurones, an NMDA receptor antagonist‐sensitive EPSP or EPSC was revealed only in magnesium‐free bathing medium, or following high‐frequency stimulation. EPSPs evoked by stimulation of the iLF exhibited a sustained potentiation of the peak amplitude (25.3 ± 11.4 %) in six of fourteen SPNs tested following a brief high‐frequency stimulus (10‐20 Hz, 0.1‐2 s). These results indicate that SPNs, including SPNs innervating the SCG, receive monosynaptic connections from both sides of the spinal cord. The neurotransmitter mediating transmission in some of the pathways activated by stimulation of iLF, cLF, cIML and DH is glutamate acting via both NMDA and non‐NMDA receptors. Synaptic plasticity is a feature of glutamatergic transmission in some SPNs where EPSPs are potentiated following a brief high‐frequency stimulus. Our data also suggest a differential expression of NMDA receptors by these neurones.

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Section: Origins Of Ipsi-and Contralaterally Evoked Monosynaptic Epspssupporting

confidence: 79%

Section: Origins Of Ipsi-and Contralaterally Evoked Monosynaptic Epspssupporting

confidence: 78%

Section: Origins Of Ipsi-and Contralaterally Evoked Monosynaptic Epspsmentioning

confidence: 58%

mentioning

confidence: 80%

mentioning

confidence: 99%

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Bilaterally evoked monosynaptic EPSPs, NMDA receptors and potentiation in rat sympathetic preganglionic neurones in vitro

Spanswick

Renaud

Logan

1998

The Journal of Physiology

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Spinal cord lamina V and lamina VII interneuronal projections to sympathetic preganglionic neurons

Cabot

Alessi

Carroll

et al. 1994

J of Comparative Neurology

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This light and electron microscopic study sought to localize spinal cord interneurons that contribute to the normal and abnormal physiological regulation of spinal sympathetic preganglionic function. Sympathetic preganglionic neurons in caudal C8 through T4 of rat spinal cord were retrogradely labeled with wheat germ agglutinin (WGA) and/or cholera beta subunit (CT beta) following injections into the superior cervical ganglion (SCG). With two exceptions, the observed locations of retrogradely WGA- and CT beta-labeled sympathetic preganglionic neurons were as expected from previous studies. The exceptions were restricted populations of cells in caudal C8 and rostral T1 spinal segments. These neurons were classified as ventrolateral (vlSPN) and ventromedial (vmSPN) sympathetic preganglionic neurons; their somata and dendrites encircled dorsolateral lamina IX motoneurons. Only WGA was transported transneuronally following the retrograde labeling of sympathetic preganglionic neurons. Transneuronally WGA-labeled spinal interneurons were located principally in the reticulated division of lamina V and dorsolateral lamina VII. A strict segmental organization was observed. All transneuronally labeled interneurons were ipsilateral to, and coextensive with, retrogradely WGA-labeled sympathetic preganglionic neurons. Electron microscopic observations suggested that retrograde transsynaptic passage of WGA occurred within the sympathetic preganglionic neuropil and showed further that similar classes of organelles were WGA immunoreactive in retrogradely labeled sympathetic preganglionic neurons and in transneuronally labeled lamina V and lamina VII neurons: 1) cisternae and vesicles at the trans face of the Golgi apparatus, 2) large endosomes/dense bodies, and 3) multivesicular bodies. The data are consistent with two hypotheses: 1) Somatic and visceral primary afferent inputs to thoracic spinal cord modify segmental sympathetic preganglionic function through activation of a disynaptic pathway involving lamina V and/or lamina VII interneurons, and 2) long-loop propriospinal pathways access sympathetic preganglionic neurons through symmetrical, segmental interneuronal circuitry.

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Sympathetic Preganglionic Neurons: Properties and Inputs

Deuchars

Lall

2015

Comprehensive Physiology

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The sympathetic nervous system comprises one half of the autonomic nervous system and participates in maintaining homeostasis and enabling organisms to respond in an appropriate manner to perturbations in their environment, either internal or external. The sympathetic preganglionic neurons (SPNs) lie within the spinal cord and their axons traverse the ventral horn to exit in ventral roots where they form synapses onto postganglionic neurons. Thus, these neurons are the last point at which the central nervous system can exert an effect to enable changes in sympathetic outflow. This review considers the degree of complexity of sympathetic control occurring at the level of the spinal cord. The morphology and targets of SPNs illustrate the diversity within this group, as do their diverse intrinsic properties which reveal some functional significance of these properties. SPNs show high degrees of coupled activity, mediated through gap junctions, that enables rapid and coordinated responses; these gap junctions contribute to the rhythmic activity so critical to sympathetic outflow. The main inputs onto SPNs are considered; these comprise afferent, descending, and interneuronal influences that themselves enable functionally appropriate changes in SPN activity. The complexity of inputs is further demonstrated by the plethora of receptors that mediate the different responses in SPNs; their origins and effects are plentiful and diverse. Together these different inputs and the intrinsic and coupled activity of SPNs result in the rhythmic nature of sympathetic outflow from the spinal cord, which has a variety of frequencies that can be altered in different conditions.

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Fast excitatory postsynaptic potentials and the responses to excitant amino acids of sympathetic preganglionic neurons in the slice of the cat spinal cord

Cited by 50 publications

References 22 publications

Bilaterally evoked monosynaptic EPSPs, NMDA receptors and potentiation in rat sympathetic preganglionic neurones in vitro

Bilaterally evoked monosynaptic EPSPs, NMDA receptors and potentiation in rat sympathetic preganglionic neurones in vitro

Spinal cord lamina V and lamina VII interneuronal projections to sympathetic preganglionic neurons

Sympathetic Preganglionic Neurons: Properties and Inputs

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