Fast differential scanning calorimetry of liquid samples with chips

Splinter, R.; Herwaarden, A.W. van; Wetten, I.A. van; Pfreundt, Andrea; Svendsen, Winnie Edith

doi:10.1016/j.tca.2014.07.013

Cited by 24 publications

(10 citation statements)

References 21 publications

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“…Recently, a gastric adenocarcinoma study offered a new graphical and numerical method for quantitating the differences among thermograms [37]. Furthermore, another study suggested a fast DSC method of liquid samples with special chips [38].…”

Section: Discussionmentioning

confidence: 99%

Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients

Zapf

Moezzi

Fekecs³

et al. 2015

J Therm Anal Calorim

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Damage caused by oxidative stress is involved in many types of diseases, including breast cancer. Our aim was to detect the oxidative stress parameters and blood plasma changes with differential scanning calorimetry (DSC) in breast cancer patients. The study included 40 adult breast cancer women who were grouped according to tumor diameter, regional lymph node metastases, proliferative activity, receptor status and postoperative chemotherapy. To monitor oxidative stress, malondialdehyde, oxygen free radicals (OFRs), activity of myeloperoxidase (MPO), superoxide dismutase (SOD) and catalase (CAT) were measured. Denaturation of plasma components was detected in Setaram Micro DSC-II calorimeter. The total production of OFRs, the MPO activity and lipidperoxidation were significantly increased in each breast cancer patients considering the tumor size, the metastatic lymph nodes, the proliferation activity and receptor status compared with healthy controls (p \ 0.05). These pro-oxidants were slightly elevated without chemotherapy, but their values were increased significantly in chemotherapy-receiving group. The activity of SOD and CAT was significantly decreased in all groups, and in regard to the chemotherapy, they were changed significantly parallel to the severity of disease. Regarding to both the increased tumor diameter and the increased number of affected lymph nodes, DSC measurements showed a strong relationship between the maximum excess heat capacity (C pmax ) of the blood plasma and the severity of disease. The study demonstrated that oxidative stress is implicated in breast carcinoma and chemotherapy aggravates these changes which confirmed the plasma DSC measurements also.

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Section: Discussionmentioning

confidence: 99%

Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients

Zapf

Moezzi

Fekecs³

et al. 2015

J Therm Anal Calorim

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“…It is likely that even in pure water, unfolding of lysozyme cannot be considered as a single-step process. One argument in favor of a multistep mechanism is based on the reported values of the activation energy of unfolding (259 kJ·mol −1 [62,63]). This is much lower than the enthalpy of lysozyme denaturation, so single-step reversible unfolding should have had a large negative activation energy in the reverse process, which is very improbable.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Dimethyl Sulfoxide on the Lysozyme Unfolding Kinetics, Thermodynamics, and Mechanism

et al. 2019

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The thermal stability of proteins in the presence of organic solvents and the search for ways to increase this stability are important topics in industrial biocatalysis and protein engineering. The denaturation of hen egg-white lysozyme in mixtures of water with dimethyl sulfoxide (DMSO) with a broad range of compositions was studied using a combination of differential scanning calorimetry (DSC), circular dichroism (CD), and spectrofluorimetry techniques. In this study, for the first time, the kinetics of unfolding of lysozyme in DMSO–water mixtures was characterized. In the presence of DMSO, a sharp decrease in near-UV CD and an increase in the fluorescence signal were observed at lower temperatures than the DSC denaturation peak. It was found that differences in the temperatures of the CD and DSC signal changes increase as the content of DMSO increases. Changes in CD and fluorescence are triggered by a break of the tertiary contacts, leading to an intermediate state, while the DSC peak corresponds to a subsequent complete loss of the native structure. In this way, the commonly used two-state model was proven to be unsuitable to describe the unfolding of lysozyme in the presence of DMSO. In kinetic studies, it was found that even high concentrations of DMSO do not drastically change the activation energy of the initial stage of unfolding associated with a disruption of the tertiary structure, while the enthalpy of denaturation shows a significant dependence on DMSO content. This observation suggests that the structure of the transition state upon unfolding remains similar to the structure of the native state.

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“…For the example given in Figure 3 (exposition time of 30 min, mercury vapor concentration of 1.5 mg/m 3 , heating temperature of 800 °C), Equation (1) delivers 3 nW. However, in the literature, for ultra-sensitive calorimetric devices detection limits around 1 μW have been reported [4]. The value estimated in Equation (1) for the mercury desorption is orders of magnitudes below this value.…”

Section: Sensor Characteristicsmentioning

confidence: 89%

Detection of Mercury Vapor in Air by Differential Heat Dissipation Measurements

Allers

Reinecke

Zimmermann

2017

Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017

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Abstract:In this work, a novel approach for the detection of mercury vapor in air is presented using differential heat dissipation measurements. Therefore, the temperature difference between an amalgamated and a non-exposed gold film is recorded, while heating both films with identical electrical power. As the amalgam layer lowers the heat dissipation, the amalgamated gold film reaches higher temperatures compared to the non-exposed gold film. Preliminary experiments show a clear dependence of the measured temperature difference on the mercury vapor concentration and the exposition time. Thus, a detection of mercury vapor is generally possible using differential heat dissipation measurements.

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Fast differential scanning calorimetry of liquid samples with chips

Cited by 24 publications

References 21 publications

Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients

Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients

The Effect of Dimethyl Sulfoxide on the Lysozyme Unfolding Kinetics, Thermodynamics, and Mechanism

Detection of Mercury Vapor in Air by Differential Heat Dissipation Measurements

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