Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography

Serafim, Jéssica Aparecida; Silveira, Raiza Felismino; Vicente, Eduardo Festozo

doi:10.1007/s12161-021-01990-w

Cited by 13 publications

(8 citation statements)

References 16 publications

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“…LOD ranged from 4.0×10 −3 to 1.9×10 −2 μM, and LOQ ranged from 1.3×10 −2 to 6.2×10 −2 μM. Compared with the method reported previously (Table 2), the sensitivity of the method established in this study was much higher than the method with HPLC‐UV [26, 27, 29] and GC‐FID [30], and a little higher than the method with GC‐MS [24, 31] and HPLC‐MS [32]. The sensitivity was lower than the method with GC‐MS/MS [25], HPLC‐MS/MS [33, 34], and HPLC‐FLD [35], but our method was more universal and the cost of the instrument was much lower.…”

Section: Resultsmentioning

confidence: 54%

A pre‐column derivatization high‐performance liquid chromatography method for simultaneous determination of short‐chain and medium‐chain fatty acids in a fecal sample

Zhang

Zeng

et al. 2022

J of Separation Science

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Short‐chain and medium‐chain fatty acids have plentiful biological functions, which play a crucial role in the diagnosis and therapy of many diseases. Herein, a new method for simultaneous quantifying 17 short‐chain and medium‐chain fatty acids with high‐performance liquid chromatography coupled with an ultraviolet detector was developed and the pre‐column derivatization by indole‐3‐acetic acid hydrazide was performed to improve the separation and detection. The conditions of the derivatization reaction were systematically investigated. Subsequently, the method was validated and the results showed a satisfactory linearity (linear regression coefficients > 0.9969), the limit of detection (4.0×10−3–1.9×10−2 μmol/L), precision (0.9%–7.3% for intra‐day and 2.0%–9.8% for inter‐day), recovery (90.0%–109.1% with relative standard deviation <7.7%) and stability (0.1%–3.3% for standard solution and 0.2%–3.9% for fecal sample). Finally, the established method was successfully applied to quantify short‐chain and medium‐chain fatty acids in the feces of healthy control and diabetic rats. Eleven kinds of short‐chain and medium‐chain fatty acids were detected and six of them showed a significant difference between the control group and the model group.

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Section: Resultsmentioning

confidence: 54%

A pre‐column derivatization high‐performance liquid chromatography method for simultaneous determination of short‐chain and medium‐chain fatty acids in a fecal sample

Zhang

Zeng

et al. 2022

J of Separation Science

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“…One millilitre of sulfuric acid (1:1) was added to a 50-mL sample of ruminal fluid and stored at −20°C for subsequent analysis of N-NH 3 using the Kjeldahl methodology (Fenner 1965). Analysis of SCFA was performed using concentrated ruminal fluid samples, following Serafim et al (2021) methodology on a Shimadzu high-performance liquid chromatography system (model Prominence) equipped with an ultraviolet detector model SPD-20 and programmed to operate at a wavelength of 210 nm. The internal standard for the high-performance liquid chromatography was the crotonic acid.…”

Section: Ruminal and Blood Parametersmentioning

confidence: 99%

Effect of diurnal feeding times and sources of energy supplementation to optimise rearing of F1 Angus × Nellore young bulls

Berça

Cardoso²,

Fonseca³

et al. 2023

Anim. Prod. Sci.

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“…The injection volume was 20 µL. The quantified VFAs were acetate, propionate, and butyrate (mmol/L) [19].…”

Section: Ruminal Parametersmentioning

confidence: 99%

Effects of Replacing Cottonseed Meal with Corn Dried Distillers’ Grain on Ruminal Parameters, Performance, and Enteric Methane Emissions in Young Nellore Bulls Reared in Tropical Pastures

Araújo

Silva

Berça

et al. 2021

Animals

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Two experiments were conducted to evaluate the effect of replacing cottonseed meal with DDG on ruminal parameters, methane (CH4) emissions (Experiment 1), and animal performance (Experiment 2) of young Nellore bulls grazing Marandu grass during the rainy season. Four supplementation strategies were used in both experiments: (1) Mineral supplementation (MS); (2) conventional multiple supplement (energy/protein) with cottonseed meal and citrus pulp (CMS); (3) CMS with 50% cottonseed meal replaced by DDG (50DDG); and (4) CMS with 100% cottonseed meal replaced by DDG (100DDG). The 50DDG condition resulted in greater intake of dry matter (p = 0.033), organic matter (OM) (p = 0.050), forage (p = 0.035), and digestible OM (p = 0.031) than 100DDG. The supplemented animals presented greater final body weight (BW) and average daily gain than the animals consuming MS (p = 0.011), and lower pH, acetate, and acetate:propionate (p < 0.05). However, the treatments had no influence on stocking rate, gain per area, and enteric CH4 emissions (p > 0.05). Replacing cottonseed meal with DDG does not result in great variations in ruminal parameters, animal performance, and enteric CH4 emissions of grazing Nellore cattle during the rearing phase in the wet season. Both protein sources in 0.3% BW supplementation can be used to intensify beef cattle production in pastures.

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Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography

Cited by 13 publications

References 16 publications

A pre‐column derivatization high‐performance liquid chromatography method for simultaneous determination of short‐chain and medium‐chain fatty acids in a fecal sample

A pre‐column derivatization high‐performance liquid chromatography method for simultaneous determination of short‐chain and medium‐chain fatty acids in a fecal sample

Effect of diurnal feeding times and sources of energy supplementation to optimise rearing of F1 Angus × Nellore young bulls

Effects of Replacing Cottonseed Meal with Corn Dried Distillers’ Grain on Ruminal Parameters, Performance, and Enteric Methane Emissions in Young Nellore Bulls Reared in Tropical Pastures

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