Fast determination of oleic acid in pork by flow injection analysis/mass spectrometry

Muñoz, Rebeca; Vilaró, F.; Eras, Jordi; Estany, J.; Tör, M.

doi:10.1002/rcm.4958

Cited by 6 publications

(5 citation statements)

References 15 publications

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“…Accordingly, no relevant changes should be expected by using on-line measurement technologies. Other direct alternative methods specifically directed to determine C18:1 content have also been proposed (Muñoz et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Expected genetic response for oleic acid content in pork1

Ros‐Freixedes

Reixach²,

Tör

et al. 2012

Journal of Animal Science

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Intramuscular fat (IMF) and oleic acid (C18:1) content in pork are important issues for the pig industry and consumers. Data from a purebred Duroc line were used to i) estimate the genetic parameters of IMF and C18:1 and their genetic correlations with lean growth components, and ii) evaluate the opportunities for genetically improving C18:1 in IMF. The data set used for estimating genetic parameters consisted of 93,920 pigs, from which 85,194 had at least 1 record for BW or backfat thickness (BT) at 180 d and 943 for IMF and C18:1 at 205 d. Intramuscular fat content and C18:1, expressed as percentage of total fatty acids, were determined in the gluteus medius muscle by gas chromatography. Genetic parameters for C18:1 were estimated under a Bayesian 4-trait multivariate animal mixed model. Heritability of C18:1 was 0.50, with a probability of 95% of being greater than 0.37. Genetic correlations of C18:1 with BW, BT, and IMF were 0.11, 0.22, and 0.47, respectively (with a probability of 95% of being greater than -0.07, 0.04, and 0.27, respectively). Genetic responses were evaluated by deterministic simulation using a half-sib recording scheme for C18:1 and the previously estimated parameters. The C18:1 content is expected to exhibit only minor changes in selection programs directed at growth rate but to decrease in those focusing on lean content. Maximum expected response in C18:1 at no lean growth loss (i.e., at no change in BW and BT) was 0.44%, with a resulting correlated response in IMF of 0.15%. However, because lean growth is emphasized in the breeding goal, the resulting response scenarios are more constrained. We concluded that there is evidence to support the idea that C18:1 in IMF is genetically determined and defined selection strategies can lead to response scenarios in which C18:1, IMF, BT, and BW can be simultaneously improved. However, if adopted, the potential for lean growth would be reduced. The extent to which it is affordable relies on how much consumers are prepared to pay for high-C18:1 pork products.

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Section: Discussionmentioning

confidence: 99%

Expected genetic response for oleic acid content in pork1

Ros‐Freixedes

Reixach²,

Tör

et al. 2012

Journal of Animal Science

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“…Como patrón interno se utilizó 1 mg de 1,2,3-Tripentadecanoil-glicerol. El análisis de los ésteres metílicos de los ácidos grasos se realizó por cromatografía gas-líquido en columna capilar (SP2330; Supelco, Tres Cantos, Madrid, España) de 30 m, un detector tipo FID, utilizando como gas portador helio a un flujo de 1 mL/minuto (Muñoz et al, 2010). La identificación de los ácidos grasos se realizó por comparación de tiempos de retención respecto a un patrón externo (F.A.M.E.…”

Section: Materials Y Métodosunclassified

Evolución a lo largo del ciclo de puesta del contenido de ácidos grasos omega-3 en huevos enriquecidos de producción ecológica

Naudi¹,

Arcas²,

Martinez³

et al. 2015

ITEA

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ResumenEn este trabajo se estudia la puesta de gallinas en producción ecológica alimentadas con pienso enriquecido con un 4% de harina de pescado y 2,95% de aceite de soja hasta las 90 semanas de vida. Se caracteriza la calidad del huevo y se estudia la evolución de la composición de su grasa. Simultáneamente se evalúa la posibilidad de simplificar el método analítico para la composición de la grasa, omitiendo el paso de extracción de la grasa, previa a la transesterificación. Las características de los huevos estudiados se corresponden con los valores bibliográficos de producción ecológica, presentando un peso medio de 57,25 ± 0,41 g, un índice de forma de 80,42 ± 0,43, un grosor de cáscara de 379,33 ± 4,56 µm, un valor de unidades Haugh de 80,17 ± 0,88 y un color en la escala DSM de entre 2 y 3. Entre la semana 1 y la semana 70 de puesta, se observa un incremento cercano al 2,2% en el porcentaje de ácidos grasos polinsaturados y un decremento equivalente en el de los monoinsaturados. El contenido de ácidos grasos ω-3 aumenta de un 2,89 ± 0,06% al inicio de la puesta, hasta un 3,2 ± 0,06% a las 70 semanas. Con respecto al método de determinación de la grasa, la transesterificación directa de la grasa sobreestimó el contenido de ácidos grasos saturados y subestimó los monoinsaturados respecto al método convencional, en un 1%. Esta diferencia, aunque significativa, en un contexto de controles de rutina, no reviste relevancia. El contenido de ácidos grasos polinsaturados, ω-3 incluidos, no se ve afectado por el método analítico.Palabras clave: Huevo, ácidos grasos, ácido docosahexaenoico, ácido eicosapentaenoico, transesterificación. Abstract Evolution of the omega-3 fatty acid content in enriched organic eggs throughout the laying periodThe evolution of the fat quality of the eggs from organic production was studied. Hens were fed a diet enriched with 4% fish meal and 2.95% soy oil during the laying period, until 90 weeks of age. Simultaneously the possibility of simplifying the fat analysis method was evaluated by omitting the step of extraction prior to transesterification of the fat. The egg quality values obtained agree with the literature on organic production, having an average weight of 57.25 ± 0.41 g, a shape rate of 80.42 ± 0.43, a shell thickness of 379.33 ± 4.56 microns, a Haugh value of 80.17 ± 0.88 units and a DSM colour score of between 2 and 3. Between 1 and 70 lay weeks, an increase of about 2.2% in the percentage of polyunsaturated fatty acids was observed and a concomitant decrease in monounsaturated fatty acid percent-* Autor para correspondencia: mtor@prodan.udl.cat http://dx.

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“…To counterbalance these limitations, the principle of flow injection analysis (FIA) has been introduced to mass spectrometry [18,19]. With regard to fatty acid analysis, Munoz et al [16] applied liquid chromatography pump for sample introduction to ESI source operated in negative mode; pork meat hydrolysates were analysed by a single quadrupole mass spectrometer achieving reliable quantification of total oleic acid. Nonetheless, the authors did not use typical FIA design because the sample was introduced continuously to the ionization source, and the correct integration of analytical signals was problematic.…”

Section: Introductionmentioning

confidence: 99%

“…Despite unquestionable advantages of gas chromatography in terms of multi‐target capability and high selectivity, relatively long separation time is a limiting factor, especially in the analysis of large sample series typically required in the quality control of commercial products. On the other hand, mass spectrometry with soft ionization sources has often been used in the analysis of triacylglycerides and fatty acids and in particular, the capability of atmospheric pressure chemical ionization (APCI) for qualitative characterization of transesterification product of coconut oil was demonstrated . As coconut oil is composed mainly by saturated fatty acids, APCI was appropriate due to its ability of efficient ionization of non‐polar compounds.…”

Section: Introductionmentioning

confidence: 99%

Determination of fatty acid methyl esters in cosmetic castor oils by flow injection–electrospray ionization–high‐resolution mass spectrometry

Negrete

Aguilar

Barrientos

et al. 2018

Intern J of Cosmetic Sci

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Objective The goal of this work was to set up a high throughput procedure for the determination of fatty acid methyl esters (FAMEs) in cosmetic castor oils using flow injection–electrospray ionization–high‐resolution mass spectrometry, and to demonstrate the need of such analysis for the quality control purposes. Methods The sample aliquot was mixed with isooctane:chloroform (1:1) and submitted to transesterification; the obtained FAMEs were appropriately diluted using water:isopropanol:acetonitrile (20:50:30) with addition of sodium formate which served as an internal standard, lock mass calibrant and promoted the formation of sodium adducts during electrospray ionization (ESI). The principle of flow injection analysis (FIA) was applied for sample introduction to an ESI–quadrupole–time of flight mass spectrometer (ESI‐QTOFMS). The carrier solution was composed of water:isopropanol:acetonitrile (20:50:30). From the acquired MS data, flowgrams of the extracted [M+Na]+ ions were obtained using the following m/z values for individual FAMEs: 293.2451 (C16:0); 315.2295 (C18:3); 317.2451 (C18:2); 319.2608 (C18:1); 321.2764 (C18:0); 335.2557 (C18:1,OH); 349.3077 (C20:0); 377.3390 (C22:0) and m/z 226.9515 for IS. Baseline‐subtracted and filtered signals were integrated, and the list of peaks intensities was exported to Excel, where calibration functions were obtained and quantification carried out. Gas chromatography with a flame ionization detector (GC‐FID) was used as an alternative analytical tool. Results The calibration detection limits for FAMEs of unsaturated fatty acids were in the range 3.61–8.62 μg L−1 and for saturated compounds in the range 8.51–82.4 μg L−1. The results obtained for commercial oils were in good agreement with GC‐FID data; among nine cosmetic oils analysed, three contained low concentrations of ricinoleic acid (C18:1, OH), indicating adulteration of castor bean oil with other vegetable oils. Conclusion Application of FIA for the sample introduction to ESI‐QTOFMS enabled for reliable determination of FAMEs in cosmetic oils with sampling frequency of thirty per hour as compared to two samples per hour achievable using GC‐FID. The proposed procedure is especially well suited for FAMEs of unsaturated fatty acids that are primary components of castor triacylglycerides and contribute to desirable properties of any cosmetic oil.

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Fast determination of oleic acid in pork by flow injection analysis/mass spectrometry

Cited by 6 publications

References 15 publications

Expected genetic response for oleic acid content in pork1

Expected genetic response for oleic acid content in pork1

Evolución a lo largo del ciclo de puesta del contenido de ácidos grasos omega-3 en huevos enriquecidos de producción ecológica

Determination of fatty acid methyl esters in cosmetic castor oils by flow injection–electrospray ionization–high‐resolution mass spectrometry

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