Fast determination of flavonoids in Hippophae rhamnoides and its medicinal preparation by capillary zone electrophoresis using dimethyl-β-cyclodextrin as modifier

Yue, Mei-E; Jiang, Tianyue; Shi, Yan‐Ping

doi:10.1016/j.talanta.2003.09.024

Cited by 64 publications

(27 citation statements)

References 16 publications

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“…Capillary electrophoresis (CE) has the advantages of excellent separation efficiency, rapid analysis and minimal use of samples and solvents for complex samples. It is an alternative technique for the separation and detection of several species of pharmaceutical importance that are extracted and used in traditional Chinese herbal medicine (Jiang et al, 2003;Yue et al, 2004Yue et al, , 2005.…”

Section: Introductionmentioning

confidence: 99%

Determination of six bioactive components of Saussurea katochaete by capillary electrophoresis

Yue

Shi

2007

Biomedical Chromatography

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The simultaneous determination of 3,4-dimethoxy-3'-hydroxy propiophenone, 5-hydroxy-7-methoxy coumarin, 7-hydroxy coumarin, 3',5'-dimethoxy apigenin, apigenin and 4-hydroxy cinnamic acid in the extract of S. katochaete has been investigated by capillary electrophoresis for the first time. The six active components were completely separated within 10 min in 20 mM Na(2)HPO(4) buffer at pH 11.00 with 10% (v/v) methanol and detected at 214 nm. The applied voltage was 15 kV and the temperature was kept at 25 degrees C. The effects of buffer pH, the concentration of Na(2)HPO(4) and the concentration of methanol on the separation efficiency were studied systematically. The regression equations revealed good linear relationships (correlation coefficients were 0.9987-0.9998) between the peak area of each analyte and its concentration. The relative standard deviations (RSD) of migration time and peak areas were <1.63 and <4.03%, respectively. The application of this method for the separation and determination of the six bioactive components in S. katochaete was reported. The contents of the six analytes ranged from 0.023 to 0.131 mg/g and recoveries ranged from 94.7 to 104.8%.

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Section: Introductionmentioning

confidence: 99%

Determination of six bioactive components of Saussurea katochaete by capillary electrophoresis

Yue

Shi

2007

Biomedical Chromatography

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“…[66][67][68][69][70][71][72][73] From the practical point of view, CE shows more advantages than other separation techniques. The capillaries attain equilibrium more easily compared with HPLC columns and the contamination problem is effectively avoided.…”

Section: ·4 Capillary Electrophoresis (Ce) Techniquesmentioning

confidence: 99%

Separation Methods for Toxic Components in Traditional Chinese Medicines

et al. 2005

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Traditional Chinese medicines (TCMs) with many unique functions for treating diseases have attracted the interest of people worldwide. They have been popularly utilized for therapy and health promotion in most Asian countries and even in many European and North American countries. However, it should be clearly noted that TCMs are mixtures with complicated composition usually containing hundreds, even thousands of chemically different constituents, and it is the multiple constituents that work synthetically to determine the ultimate effect of a formula of TCM. Meanwhile, some components with toxicity in some TCMs, having various negative effects on different parts of body, may do serious harm to people's health; such harm in particular requires our attention. In this article, applications of different chromatographic and electrophoretic techniques in the analysis of toxic components in TCMs in recent decades have been comprehensively reviewed and some hyphenated procedures (combinations of two kinds of measurement) applied in this field are also summarized.

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“…Because of the bioenhancing effects of flavonoids on drugs and nutriments, the determination of flavonoids in Saussurea species will play an essential part in research on the efficacy, safety, and therapeutic reproducibility of this medicinal plant. Several assays have been described for the determination of flavonoids based on gas chromatography (GC) [13], thin layer chromatography (TLC) [14], high-performance liquid chromatography with ultra-violet detection (HPLC-UV) [15,16], HPLC with evaporative light scattering detection (HPLC-ELSD) [17], capillary electrophoresis (CE) [18], gas chromatography with mass spectrometry (GC-MS) [19], HPLC with mass spectrometry (HPLC-MS) [20,21], and with nuclear magnetic resonance spectroscopy (HPLC-NMR) [22].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of five flavonoids inSaussurea mongolicaFranch by high-performance liquid chromatography

Chen

Shi

2012

Acta Chromatographica

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Summary.A high-performance liquid chromatographic (HPLC) technique coupled with photodiode array (PDA) detection has been proposed for simultaneous determination of five flavonoids, i.e. quercetin 3-O-β-D-glucopyranoside, quercetin 4′-methoxy-3-O-β-Dgalactopyranoside, kaempferol 3-O-α-L-rhamnopyranoside, asebotin, and kaempferol 7-methxoy-3-O-α-L-rhamnopyranoside in extract of the whole plant of Saussurea mongolica Franch. The optimum conditions for separation were achieved on a 4.6 × 250 mm i.d., 5-μm particle, C 18 column with acetonitrile and 1% acetic acid (20:80, v/v) as the mobile phase at a flow rate of 1.0 mL min −1 . For all the analytes, a good linear regression relationship (r of >0.999) was obtained between peak area and concentration over a relatively wide range. The method was validated for repeatability, precision, stability, and accuracy. Seven different extraction procedures were investigated for preparation of the sample solution. The validated method was successfully applied to simultaneous analysis of these flavonoids in S. mongolica and was found to be simple and efficient.

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Fast determination of flavonoids in Hippophae rhamnoides and its medicinal preparation by capillary zone electrophoresis using dimethyl-β-cyclodextrin as modifier

Cited by 64 publications

References 16 publications

Determination of six bioactive components of Saussurea katochaete by capillary electrophoresis

Determination of six bioactive components of Saussurea katochaete by capillary electrophoresis

Separation Methods for Toxic Components in Traditional Chinese Medicines

Simultaneous determination of five flavonoids inSaussurea mongolicaFranch by high-performance liquid chromatography

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