Fast Biodegradation of Diesel Hydrocarbons at High Concentration by the Sophorolipid-Producing Yeast <b><i>Candida catenulata</i></b> KP324968

Abstract

In the last decades, biodegradation as an environmentally friendly approach has raised interest in connection with the removal of hydrocarbon pollutants. Its capacity for removing pollutants strongly depends on the type of living cell and environmental conditions. The degradative activity of a new sophorolipid-producing yeast, Candida catenulata KP324968, in the removal of high concentrations of diesel from effluents was statistically evaluated considering the initial pH, the agitation speed, and the initial diesel concentration. The optimal setting of the operational variables at an initial pH of 4.7, an agitation speed of 204 rpm, and an initial diesel concentration of 93.4 g L^–1 resulted in the highest total petroleum hydrocarbon removal efficiency: about 82.1% after 6 days (biodegradation rate: 0.378 g g_cell^–1 h^–1). During the cell growth phase, the emulsification index in the medium increased and reached its highest level at 64.6% after 48 h. Further tests indicated that the emulsification capacity was obtained by in situ production of two sophorolipid molecules with an m/z of 533 and 583. In summary, its effective diesel removal and high emulsification capacity makes C. catenulata KP324968 an attractive candidate yeast for the degradation of hydrocarbons from aqueous environments.

Role of Guinea Pigs (Cavia porcellus) Raised as Livestock in Ecuadorian Andes as Reservoirs of Zoonotic Yeasts

“…Diutina catenulata (formerly Candida catenulata ), an ascomycete closely related to Saccharomyces cerevisiae and often used for bioremediation purposes [ 38 , 39 ], was also present in the nasal mucosa of the healthy guinea pigs studied. This species has been previously isolated from the gut of birds [ 20 , 40 , 41 ] and the nasolacrimal duct of healthy horses [ 18 ].…”

Free fatty acids in sunflower oil soap stock progressed the sophorolipid production by Candida catenulata

“…LC-MS analysis was performed using an Agilent 1200 series LC system equipped with an Agilent 6410 Triple Quadrupole mass spectrometer (Agilent Technologies, Palo Alto, CA, USA). Ion-ionization was achieved using electrospray ionization in the negative mode with a capillary voltage of 4000 V. [19] Nitrogen was heated to 300 C and used as the nebulizer gas at a flow rate of 10 L min À1 , with a nebulizer pressure of 40 psi.…”

From bumblebee to bioeconomy: Recent developments and perspectives for sophorolipid biosynthesis

“…Lubricating oil contaminated soils have been treated by SLs by Minucelli et al (2017), but most of the research has been successfully conducted towards remediation and dispersion of petroleum derived hydrocarbon fractions such as 2-methylnaphthalene, hexadecane, pristane, (bio)diesel, aviation kerosene and (light) crude oil phases itself in (sea)water, beach sands and soils (Babaei and Habibi, 2018;Goswami et al, 2020;Kang et al, 2010;Saborimanesh and Mulligan, 2018). The concepts described above are also seen during biosurfactant or sophorolipid enhanced anaerobic fermentation in wastewater treatment as a consequence of increased solubility of the waste activated sludge, enhanced release of biodegradable organics and favoured growth of hydrolytic microbes and short-chain fatty acid producers over present methanogens (Huang et al, 2015;Xu et al, 2019).…”