Fast axonal transport of foreign transmitters in an identified serotonergic neurone of Aplysia californica.

Goldberg, Daniel J.; Schwartz, James H.

doi:10.1113/jphysiol.1980.sp013434

Cited by 14 publications

(5 citation statements)

References 32 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It also suggests that, over the period of these experiments (2-3 d), a significant proportion of % in methionine could be transferred to cysteine, either free or in GSSG. The rapid diffusion of small molecules down axons has been described previously in Aplysia (Koike and Nagata, 1979;Goldberg and Schwartz, 1980).…”

Section: Resultsmentioning

confidence: 65%

Fast axonal transport of modulatory neuropeptides from central ganglia to components of the feeding system in Aplysia

Lloyd

1988

J. Neurosci.

View full text Add to dashboard Cite

The transport of neuropeptides from central ganglia to components of the feeding system was studied in Aplysia. Peptide transport was determined by incubating buccal or cerebral ganglia with 35S-methionine and measuring the appearance of labeled peptides by high-pressure liquid chromatography (HPLC) of extracts of target tissues. Selected nerves were left intact and passed through a Vaseline diffusion barrier separating the ganglia and their targets. Five major labeled peptides were observed to be transported from the buccal ganglia to feeding muscles. They were buccalin, FMRFamide, myomodulin, and 2 small cardioactive peptides. Each of these peptides has been shown to modulate the responses of these muscles to their motor neurons. The peptides were transported by fast axonal transport, as judged by the distance transported and the sensitivity to colchicine. When normalized to correct for differences in total incorporation, the patterns of peptide transport were reproducible between animals. The nature and amount of the peptides transported were different for different muscles. The nature of peptide transport also varied for different nerve groups. These results support the proposition that these 5 peptides act as modulatory transmitters at feeding muscles. No transport of neuropeptides from the cerebral ganglia to feeding muscles was observed, although myomodulin was specifically transported to the buccal ganglia. This suggests that this peptide may play an important role in the previously observed regulation of buccal ganglia activity by neurons in the cerebral ganglia.

show abstract

Section: Resultsmentioning

confidence: 65%

Fast axonal transport of modulatory neuropeptides from central ganglia to components of the feeding system in Aplysia

Lloyd

1988

J. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Selective accumulation of labeled histamine, but not its metabolite, occurred proximal to a ligature on the posterior lip nerve axon ; little was measured distal to the ligature (Gotoh & Schwartz 1982). In contrast, the serotonergic Cl cell transported histamine, dopamine, octopamine and 5-hydroxytryptamine at about the same rates (Goldberg & Schwartz 1980). Subcellular fractionation showed one-third of the labeled histamine in particulate fractions as early as 3 hr after injection.…”

Section: Nervous Systemmentioning

confidence: 94%

Histamine as a Neuroregulator

Prell¹,

Green²

1986

Annu. Rev. Neurosci.

263

View full text Add to dashboard Cite

“…, the average distance between each that in marking the synapse for synaptic growth, 5-HT, cAMP, and PKA act like growth factors. Growth fac-sensory cell synapse and nucleus is 500 m. From the rate of fast retrograde and anterograde axonal transport tors are known to stimulate protein synthesis and frequently do so by means of a particular signal transduc-in Aplysia neurons(Goldberg and Schwartz, 1980), one can estimate that approximately 15 min would be tion pathway that is sensitive to the immunosuppresant rapamycin (Brown and Schreiber, 1996). 5-HT-induced needed for the retrograde signal to reach the nucleus, and only 6-15 min would be needed for the products of increases in translation have been shown to be rapamycin sensitive in dissected Aplysia pleural ganglia gene expression to be transported from the nucleus back to the synapse.…”

mentioning

confidence: 99%

A Transient, Neuron-Wide Form of CREB-Mediated Long-Term Facilitation Can Be Stabilized at Specific Synapses by Local Protein Synthesis

Casadio

Martin

Giustetto

et al. 1999

Cell

460

441

View full text Add to dashboard Cite

In a culture system where a bifurcated Aplysia sensory neuron makes synapses with two motor neurons, repeated application of serotonin (5-HT) to one synapse produces a CREB-mediated, synapse-specific, long-term facilitation, which can be captured at the opposite synapse by a single pulse of 5-HT. Repeated pulses of 5-HT applied to the cell body of the sensory neuron produce a CREB-dependent, cell-wide facilitation, which, unlike synapse-specific facilitation, is not associated with growth and does not persist beyond 48 hr. Persistent facilitation and synapse-specific growth can be induced by a single pulse of 5-HT applied to a peripheral synapse. Thus, the short-term process initiated by a single pulse of 5-HT serves not only to produce transient facilitation, but also to mark and stabilize any synapse of the neuron for long-term facilitation by means of a covalent mark and rapamycin-sensitive local protein synthesis.

show abstract

Fast axonal transport of foreign transmitters in an identified serotonergic neurone of Aplysia californica.

Cited by 14 publications

References 32 publications

Fast axonal transport of modulatory neuropeptides from central ganglia to components of the feeding system in Aplysia

Fast axonal transport of modulatory neuropeptides from central ganglia to components of the feeding system in Aplysia

Histamine as a Neuroregulator

A Transient, Neuron-Wide Form of CREB-Mediated Long-Term Facilitation Can Be Stabilized at Specific Synapses by Local Protein Synthesis

Contact Info

Product

Resources

About