Fast atom bombardment and tandem mass spectrometry of covalently modified nucleosides and nucleotides: adducts of pyrrolizidine alkaloid metabolites

Mass spectrometry for the analysis of carcinogen‐DNA adducts

“…93 the proposed structure for the product ion at mass 136 corresponded to a radical cation. An extensive study of the tandem mass spectra of 12 different nucleoside and nucleotide adducts of pyrrolizidine metabolites, also using FAB ionization, did not support this proposed fragmentation pathway nor the radical cation structure (94). However, as noted in Sec.…”

Molecular recognition and supramolecular chemistry in the gas phase

“…Most of the early studies are concerned with an understanding of the fragmentation of the alkali ion oligonucleoside or oligonucleotide complexes, aiming at sequence determination (Hogg et al, 1986;Tomer et al, 1986;Rodgers et al, 1997). Also, the location of the alkali metal ion has been a topic, and co-ordination of K þ to the nucleobase rather than the backbone has been suggested (Tomer et al, 1986). A size dependence of the intensities of [nucleotide þ M] þ was found in that the smaller alkali metal ion gives more intense signals (Voyksner, 1987).…”

Mass spectrometry research: An interplay between ion chemistry, instrumental development, and applications

“…With Professor M. Deinzer (Oregon State), we investigated the CAD spectra of 12 nucleoside and nucleotide adducts that were modified by bonding to various pyrrolizidine alkaloid metabolites (40). The alkaloids are carcinogens and mutagens and are a threat to both livestock, who feed off plants containing the alkaloids, and possibly to man.…”