Mass spectrometry research: An interplay between ion chemistry, instrumental development, and applications

Electrospray ionization with low-energy collisionally activated dissociation tandem mass spectrometry of glycerophospholipids: Mechanisms of fragmentation and structural characterization

“…Structural characterization achieved by magnetic sector instruments, which involve high-energy collisionally activated dissociation (CAD) using keV translational energy [20, 21], may yield additional structural information [22], but the sensitivity is poor, as compared to that obtained by TSQ, which is usually operated with collision energies below 100 eV [23]. In addition, with the relative ease in applying skimmer CAD, MS/MS/MS experiment can be carried out by TSQ to reveal the fragmentation processes in greater detail.…”

Fast atom bombardment mass spectrometry of phospholipids

“…The carboxylate anions generated either in the ion source or following collision induced decomposition of the [M -151-ion of glycerophosphocholine lipids have been further studied by tandem mass spectrometry using high-energy collisional activation, as described by 38). Activation of these carboxylate anions results in a charge-site-remote rearrangement and subsequent decomposition, which yields specific structural information concerning the nature of the fatty acyl group (42,43). This approach has been used to characterize the position of unsaturation in an unusual 26:2 fatty acyl group esterified to a sponge glycerophospholipid (44).…”

Determination of Alkenes in Hydrocarbon Matrices by Acetone Chemical Ionization Mass Spectrometry

“…A faster and more sensitive approach is to selectively ionize the olefinic structures in the chemical ionization (CI) source of a mass spectrometer by ion−molecule reactions. Such an approach for the characterization of alkenes has been the reaction of alkenes with Fe + as reagent ion. − Unfortunately, this technique is not highly suitable for routine analysis due to the long-term adverse effects of the metal reagent ion on the ionization source and the difficulties of the approach associated with gas chromatography/mass spectrometry (GC/MS) analysis. , The use of organic reagent compounds that are less aggressive to the ionization source and the vacuum system would be more suitable for the routine analysis of alkenes in petroleum fractions.…”