Fast and Universal HPLC Method for Determination of Permethrin in Formulations Using 1.8- m Particle-Packed Column and Performance Comparison with Other Column Types

Abstract

An HPLC method has been developed for the fast separation and quantification of permethrin using C18 column packed with 1.8 µm particles. The method is specific with good resolution to degradation products and to other present components. It has acceptable validation results. The run time is 4.5 min (or may be within 1.6 min is rapid resolution mode) with an organic solvent consumption of 3.6 mL per run. The method has been applied to samples of formulations for various uses: mattress cleaner, shampoo, and veterinary powder. The performance of the applied column is compared with other common column types. The relationships between linear velocity of the mobile phase (u) and resolution factor (Rs), back-pressure (ΔP), and efficiency (H) are presented. The experimental data shows the advantages of 1.8-µm particle columns to be a significant reduction in solvent consumption (by factor of 4.4 and 1.5) and a reduction in run-time (by factor 4.7 and 1.5), and the weaknesses are a high back-pressure and lower efficiency. Finally, it has been shown that use of 1.8-µm particle packed columns with conventional HPLC systems is possible, but with limitations in mobile phase flow-rate.

Permethrin exposure affects neurobehavior and cellular characterization in rats' brain

“…The increase in the use of permethrin as a pesticide to manage household insects that constitute nuisance and pose health risk such as malaria is alarming [26,27]. Despite being a very effective insecticide/pesticide [28,29], there are increasing evidences on the toxicity tendency of permethrin [30][31][32].…”

An Ecofriendly and Stability‐Indicating HPLC Method for Determination of Permethrin Isomers: Application to Pharmaceutical Analysis

“…e LOQ of permethrin was determined to be 0.5 g/mL, considering the mean accuracy value of 101.18% and maximum RSD value of 2.07% (Table 1). is value indicates that the proposed method is much more sensitive than what have been reported previously [12][13][14][15][16].…”

“…In addition, recycling signi�cantly reduced the mobile phase consumption and made the method economic. Moreover, the method is much more sensitive than the previously reported procedures [12][13][14][15][16].…”

“…However, no method was compiled in British and United state pharmacopoeias for the analysis of this medicine in pharmaceutical preparations. Few methods reported the quantitation of permethrin in pharmaceutical dosage forms using spectrophotometric methods, [12] and LC methods with ultraviolet detection [13][14][15][16], most of which have not been validated according to International Conference on Harmonization (ICH) stability-indicating guidelines.…”

An analytical approach for extraction and detection of permethrin from functionalized textile materials

“…[7]. For permethrin analysis are reported several methods based on separation techniques followed by identification and quantification using different types of detectors (GC-FID [8], GC-ECD [9], GC-MS [10], HPLC-DAD [11], HPLC-UV [12][13]. The challenging in permethrin testing is to establish the suitable HPLC chromatographic conditions that ensure complete resolution of the two geometric isomers.…”