Fast and sensitive supercritical fluid chromatography – tandem mass spectrometry multi-class screening method for the determination of doping agents in urine

Recent Advances in Chromatography for Pharmaceutical Analysis

“…In addition to the excellent retention of the most polar drugs and its complementarity with RPLC, it appears that the sensitivity of SFC-MS/MS was equivalent to that of RPLC-MS/MS for 46% of the compounds and the sensitivity was improved in SFC-MS/MS for 32% of the compounds. 62 In another study related to doping control analysis, most of the investigated anabolic agents, synthetic cannabinoids and glucocorticoids, were detectable at very low concentrations (below 0.1 ng/mL) in urine by SFC-MS. 63 In recent times, various research groups have worked to evaluate matrix effects (MEs) in SFC-MS, since it remains one of the major drawbacks of quantitative MS-based bioanalytical methods. Desfontaine et al [64][65][66] systematically compared the incidences of ME in RPLC-MS and SFC-MS for a wide range of drugs in urine and plasma matrices using three different SFC column chemistries with nonselective and selective sample preparation procedures.…”

Annual banned‐substance review: analytical approaches in human sports drug testing

“…In addition, the performance of the assay targeting recently identified long‐term metabolites of AAS would be of particular interest to outline the fitness‐for‐purpose of this alternative to established chromatographic‐mass spectrometric test methods. Also utilizing an orthogonal chromatographic system, Novakova et al . as well as Desfontaine et al .…”

“…In addition, the performance of the assay targeting recently identified long-term metabolites of AAS would be of particular interest to outline the fitness-for-purpose of this alternative to established chromatographic-mass spectrometric test methods. Also utilizing an orthogonal chromatographic system, Novakova et al [54] as well as Desfontaine et al [55] demonstrated the capability of supercritical fluid (SFC) LC interfaced via positive ESI to a QqQ-based MS/MS system to detect steroidal analytes in human urine. In both studies, assays were developed that employed identical instruments consisting of an SFC liquid chromatograph equipped with a so-called high density diol analytical column (100 x 3.0 mm, 1.7 μm particle size) operated with mobile phases composed of CO 2 and methanol/water (49:1, v/v) containing 10 mM ammonium formate.…”

Enantioselective potential of polysaccharide‐based chiral stationary phases in supercritical fluid chromatography

“…1,10 From this point of view, SFC offers benefits such as higher throughput or lower analysis times than the conventional high-performance liquid chromatography (HPLC) technique. 1,[11][12][13] On the market, there are a number of chiral stationary phases (CSPs) including many of HPLC CSPs, that are suitable for enantioseparation in SFC. Regarding HPLC as well as SFC enantioseparations, polysaccharide-based CSPs are classified as the versatile ones and are extensively used.…”

“…1,10 From this point of view, SFC offers benefits such as higher throughput or lower analysis times than the conventional high-performance liquid chromatography (HPLC) technique. 1,[11][12][13] [This article is part of the Special Issue: Proceedings of 28th International Symposium on Molecular Chirality, Heidelberg 2016. See the first articles for this special issue previously published in Volumes 29:1, 29:3-4, and 29:5.…”