Fast and sensitive GCaMP calcium indicators for imaging neural populations

Zhang, Yan; Rózsa, Márton; Liang, Yajie; Bushey, Daniel; Wei, Ziqiang; Zheng, Jihong; Reep, Daniel; Broussard, Gerard Joey; Tsang, Arthur; Tsegaye, Getahun; Narayan, Sujatha; Obara, Christopher J.; Lim, Jing-Xuan; Patel, Ronak; Zhang, Rongwei; Ahrens, Misha B.; Turner, Glenn; Wang, Samuel S.‐H.; Korff, Wyatt; Schreiter, Eric R.; Svoboda, Karel; Hasseman, J.; Kolb, Ilya; Looger, Loren L.

doi:10.1038/s41586-023-05828-9

Cited by 211 publications

(212 citation statements)

References 71 publications

(156 reference statements)

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“…As new GCaMP variants continue to be developed and improved, the plasmids provided by our toolkit can be used as a foundation to produce additional C. elegans optimized plasmids and be further mutated to a desired new GCaMP coding sequence. For example, the GENIE group at the HHMI Janelia research campus has recently developed their next generation jGCaMP8 series of reporters (ZHANG et al . 2021).…”

Section: Resultsmentioning

confidence: 99%

An expanded GCaMP reporter toolkit for functional imaging inC. elegans

Ding

Peng

Moon

et al. 2023

Preprint

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In living organisms, changes in calcium flux are integral to many different cellular functions and are especially critical for the activity of neurons and myocytes. Genetically encoded calcium indicators (GECIs) have been popular tools for reporting changes in calcium levelsin vivo. In particular, GCaMP, derived from GFP, are the most widely used GECIs and have become an invaluable toolkit for neurophysiological studies. Recently, new variants of GCaMP, which offer a greater variety of temporal dynamics and improved brightness, have been developed. However, these variants are not readily available to theCaenorhabditis elegansresearch community. This work reports a set of GCaMP6 and jGCaMP7 reporters optimized forC. elegansstudies. Our toolkit provides reporters with improved dynamic range, varied kinetics, and targeted subcellular localizations. Besides optimized routine uses, this set of reporters are also well-suited for studies requiring fast imaging speeds and low magnification or low-cost platforms.

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Section: Resultsmentioning

confidence: 99%

An expanded GCaMP reporter toolkit for functional imaging inC. elegans

Ding

Peng

Moon

et al. 2023

Preprint

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“…Current approaches are based on nuclear-targeted calcium indicators which have relatively slow temporal dynamics, with decay lasting several seconds (though see Zhang et al, 2023 ). This makes correlating behavior with neural activity particularly challenging, given that the timescale of sensorimotor integration during hunting events is at least an order of magnitude faster than this.…”

Section: Discussionmentioning

confidence: 99%

Whole-brain imaging of freely-moving zebrafish

et al. 2023

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One of the holy grails of neuroscience is to record the activity of every neuron in the brain while an animal moves freely and performs complex behavioral tasks. While important steps forward have been taken recently in large-scale neural recording in rodent models, single neuron resolution across the entire mammalian brain remains elusive. In contrast the larval zebrafish offers great promise in this regard. Zebrafish are a vertebrate model with substantial homology to the mammalian brain, but their transparency allows whole-brain recordings of genetically-encoded fluorescent indicators at single-neuron resolution using optical microscopy techniques. Furthermore zebrafish begin to show a complex repertoire of natural behavior from an early age, including hunting small, fast-moving prey using visual cues. Until recently work to address the neural bases of these behaviors mostly relied on assays where the fish was immobilized under the microscope objective, and stimuli such as prey were presented virtually. However significant progress has recently been made in developing brain imaging techniques for zebrafish which are not immobilized. Here we discuss recent advances, focusing particularly on techniques based on light-field microscopy. We also draw attention to several important outstanding issues which remain to be addressed to increase the ecological validity of the results obtained.

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“…The method reported in this study could activate the Ca 2+ activities of any target individual cochlear cell. Recently, Ca 2+ fluorescent indicators have been widely used in neuroscience as an alternative to electrical activity patterns. − Many cells can be recorded simultaneously using time-lapse microscopy of Ca 2+ fluorescence, which could be located exactly at each individual neuron.…”

Section: Discussionmentioning

confidence: 99%

Store-Operated Ca²⁺ Channels Contribute to the Generation of Ca²⁺ Waves in Interdental Cells in the Cochleae

Zhang

et al. 2023

ACS Chem. Neurosci.

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Cochlear calcium (Ca2+) waves are vital regulators of the cochlear development and establishment of hearing function. Inner supporting cells are believed to be the main region generating Ca2+ waves that work as internal stimuli to coordinate the development of hair cells and the mapping of neurons in the cochlea. However, Ca2+ waves in interdental cells (IDCs) that connect to inner supporting cells and spiral ganglion neurons are rarely observed and poorly understood. Herein, we reported the mechanism of IDC Ca2+ wave formation and propagation by developing a single-cell Ca2+ excitation technology, which can easily be accomplished using a two-photon microscope for simultaneous microscopy and femtosecond laser Ca2+ excitation in any target individual cell in fresh cochlear tissues. We demonstrated that the store-operated Ca2+ channels in IDCs are responsible for Ca2+ wave formation in these cells. The specific architecture of the IDCs determines the propagation of Ca2+ waves. Our results provide the mechanism of Ca2+ formation in IDCs and a controllable, precise, and noninvasive technology to excite local Ca2+ waves in the cochlea, with good potential for research on cochlear Ca2+ and hearing functions.

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Fast and sensitive GCaMP calcium indicators for imaging neural populations

Cited by 211 publications

References 71 publications

An expanded GCaMP reporter toolkit for functional imaging inC. elegans

An expanded GCaMP reporter toolkit for functional imaging inC. elegans

Whole-brain imaging of freely-moving zebrafish

Store-Operated Ca²⁺ Channels Contribute to the Generation of Ca²⁺ Waves in Interdental Cells in the Cochleae

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Fast and sensitive GCaMP calcium indicators for imaging neural populations

Cited by 211 publications

References 71 publications

An expanded GCaMP reporter toolkit for functional imaging inC. elegans

An expanded GCaMP reporter toolkit for functional imaging inC. elegans

Whole-brain imaging of freely-moving zebrafish

Store-Operated Ca2+ Channels Contribute to the Generation of Ca2+ Waves in Interdental Cells in the Cochleae

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Store-Operated Ca²⁺ Channels Contribute to the Generation of Ca²⁺ Waves in Interdental Cells in the Cochleae