Fast and label-free

Rigneault, Hervé; Andresen, Esben Ravn

doi:10.1038/nphoton.2012.301

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…Raman scattering. Raman scattering is an inelastic phenomenon resulting from energy exchange between excitation photons and scattering molecules generating the vibration of chemical bonds [59]. Raman spectroscopy gives information about the density of chemical bonds of a single functional group.…”

Section: Theory Of Multiphoton Processes and Technical Requirements F...mentioning

confidence: 99%

A review of biomedical multiphoton microscopy and its laser sources

Lefort¹

2017

J. Phys. D: Appl. Phys.

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Multiphoton microscopy (MPM) has been the subject of major development efforts for about 25 years for imaging biological specimens at micron scale and presented as an elegant alternative to classical fluorescence methods such as confocal microscopy. In this topical review, the main interests and technical requirements of MPM are addressed with a focus on the crucial role of excitation source for optimization of multiphoton processes. Then, an overview of the different sources successfully demonstrated in literature for MPM is presented, and their physical parameters are inventoried. A classification of these sources in function with their ability to optimize multiphoton processes is proposed, following a protocol found in literature. Starting from these considerations, a suggestion of a possible identikit of the ideal laser source for MPM concludes this topical review.

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Section: Theory Of Multiphoton Processes and Technical Requirements F...mentioning

confidence: 99%

A review of biomedical multiphoton microscopy and its laser sources

Lefort¹

2017

J. Phys. D: Appl. Phys.

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“…However, these systems are usually based on expensive short pulse laser sources which require elaborate techniques for hyperspectral recordings and fiber delivery is very challenging due to non-linear effect s in fiber -especially self-phase modulation). Moreover, different characteristics of vibrational modes in the region of 3000 cm -1 and the fingerprint region from 700 cm -1 to 1500 cm -1 demand flexible spectrum acquisition, which increase s the cost of short pulse system [18].…”

Section: Introductionmentioning

confidence: 99%

Hyperspectral stimulated Raman microscopy with two fiber laser sources

et al. 2015

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A fast all fiber based setup for stimulated Raman microscopy based on a rapidly wavelength swept cw-laser is presented. The applied Fourier domain mode locked (FDML) laser is a fiber ring laser, providing a continuously changing wavelength output over time. This fast swept source allows us to rapidly change the wavelength and, thereby the energy difference with respect to a single color pump laser. The pump laser is a master oscillator power amplifier based on a fiber amplified laser diode and a Raman shifter. By controlled variation of the relative timing between probe and pump laser with an arbitrary waveform generator, the Raman signals are encoded in time and they are directly acquired with a synchronized, fast analog-to-digital converter. This setup is capable of acquiring rapidly high resolution spectra (up to 0.5 cm -1 ) with shot noise limited sensitivity over a broadband (750 cm -1 to 3150 cm -1 ) spectral region. Here, we show the performance of this system for imaging in the CH-stretch region around 3000 cm -1 and in the fingerprint r egion around 1600 cm -1 . We present hyperspectral images of a plant stem slice with molecular contrast of lignin and a lipid representative as well as images of PS (polystyrene) and PMMA (poly(methyl methacrylate) beads with an acquisition speed of 18 µs per spectral point.

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“…Recent efforts concentrate on fiber delivery (13)(14)(15)(16) for future endoscopic applications (17), and cost effective continuous wave (CW) systems (18)(19)(20). However, the demand for a flexible, broadband, high resolution Raman imaging system based on fiber light sources is still unmet (21). We present a new SRS technique based on fastest wavelength-swept CW probe lasers.…”

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confidence: 99%

A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy

et al. 2015

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Raman sensing and microscopy are among the most specific optical technologies to identify the chemical compounds of unknown samples, and to enable label-free biomedical imaging. Here we present a method for stimulated Raman scattering spectroscopy and imaging with a time-encoded (TICO) Raman concept. We use continuous wave, rapidly wavelength-swept probe lasers and combine them with a short-duty-cycle actively modulated pump laser. Hence, we achieve high stimulated Raman gain signal levels, while still benefitting from the narrow linewidth and low noise of continuous wave operation. Our all-fibre TICO-Raman setup uses a Fourier domain mode-locked laser source to achieve a unique combination of high speed, broad spectral coverage (750–3,150 cm−1) and high resolution (0.5 cm−1). The Raman information is directly encoded and acquired in time. We demonstrate quantitative chemical analysis of a solvent mixture and hyperspectral Raman microscopy with molecular contrast of plant cells.

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Fast and label-free

Cited by 6 publications

References 7 publications

A review of biomedical multiphoton microscopy and its laser sources

A review of biomedical multiphoton microscopy and its laser sources

Hyperspectral stimulated Raman microscopy with two fiber laser sources

A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy

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