Fasciola hepatica: Histology of the testis in egg-producing adults of several laboratory-maintained isolates of flukes grown to maturity in cattle and sheep and in flukes from naturally infected hosts

Hanna, R.E.B.; Edgar, H.W.J.; Moffett, D.; McConnell, S.; Fairweather, Ian; Brennan, Gerard; Trudgett, Alan; Hoey, Elizabeth M.; Cromie, L.; Taylor, Subhashni; Daniel, Roger

doi:10.1016/j.vetpar.2008.07.022

Cited by 35 publications

(34 citation statements)

References 10 publications

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“…The histological features of the testis tubules in untreated flukes of the Cullompton isolate were consistent with the description given by Hanna et al (2008;Fig. 2a-d).…”

Section: Histologysupporting

confidence: 88%

“…The Cullompton isolate of F. hepatica was used for the studies on compound alpha because it is known to be susceptible to the action of triclabendazole (for references, see Materials and methods). It should perhaps be pointed out that this isolate is aspermic, spermatogenesis not proceeding beyond the primary spermatocyte stage (Fletcher et al 2004;Hanna et al 2008). In all other respects, the morphology of this isolate is normal, and it can produce viable eggs.…”

Section: Introductionmentioning

confidence: 83%

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Fasciola hepatica: disruption of spermatogenesis by the fasciolicide compound alpha

et al. 2009

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Sheep infected with the triclabendazole-susceptible Cullompton isolate of Fasciola hepatica were dosed with 15 mg/kg of compound alpha at 12 weeks post-infection. Adult flukes were recovered from the bile ducts at 24, 48, and 72 h post-treatment (p.t.). Changes to the spermatogenic cells in the testis were examined by histology and transmission electron microscopy. Disruption to the testes became increasingly severe over time. The testis tubules shrank in size, became vacuolated, and contained fewer cells. Identification of cell types became difficult, and apoptotic eosinophilic bodies were the predominant feature at 72 h p.t. Changes to the spermatogonia were evident at 24 h p.t., the cells containing swollen and electron-lucent mitochondria. The proportion of tertiary spermatogonia increased at 48 h p.t., and they showed signs of autophagy. Multinucleate spermatogonia were a feature of drug treatment at this time point, and they contained autophagic vacuoles. By 72 h p.t., it was difficult to identify primary and secondary spermatogonia, and there were no recognisable clusters of tertiary spermatogonia. Most spermatogonial cells were multinucleate and in the process of breaking down. With regard to the primary spermatocytes, fragmentation of the cytophore was observed at 24 h p.t. Intact rosettes were rare after 48 h treatment; collections of cells were seen, but were not organised into clusters. By 72 h p.t., no spermatocyte cells could be recognised. The results indicate that spermatogenesis was severely affected by compound alpha.

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“…The histological features of the testis tubules in untreated flukes of the Cullompton isolate were consistent with the description given by Hanna et al (2008;Fig. 2a-d).…”

Section: Histologysupporting

confidence: 88%

Section: Introductionmentioning

confidence: 83%

Fasciola hepatica: disruption of spermatogenesis by the fasciolicide compound alpha

et al. 2009

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“…Following OZ78 treatment, the spermatogonia were abnormal and the primary spermatocytes were multinucleate, as a result of nuclear, but not cytoplasmic, division. Eosinophilic and apoptotic multinucleate bodies equivalent to aberrant secondary spermatocytes have been described by Hanna et al (2008), but were not seen in the present study. So, even within the limited amount of sperm development that this isolate undergoes, there was disruption of the two early stages and the follicle became very empty.…”

Section: Discussioncontrasting

confidence: 76%

“…In the testes, only spermatogonia and primary spermatocytes were present and in low numbers. The fluke isolate used for this experiment was the Cullompton isolate : in this isolate, spermatogenesis is known not to proceed beyond the primary spermatocyte stage, with the consequence that this isolate is aspermic and parthenogenetic (Fletcher et al 2004 ;Hanna et al 2008). Following OZ78 treatment, the spermatogonia were abnormal and the primary spermatocytes were multinucleate, as a result of nuclear, but not cytoplasmic, division.…”

Section: Discussionmentioning

confidence: 99%

Electron microscopical study to assess thein vitroeffects of the synthetic trioxolane OZ78 against the liver fluke,Fasciola hepatica

et al. 2009

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Adult Fasciola hepatica were incubated for 48 h in vitro in the synthetic peroxide, OZ78 at a concentration of 100 mg/ml and then prepared for scanning and transmission electron microscopy. There was limited disruption to the external fluke surface, with only slight swelling and blebbing of the interspinal tegument in the midbody and ventral tail regions. By contrast, significant disruption was observed to the ultrastructure of the tegument and subtegumental tissues. There was severe swelling of the basal infolds in the tegumental syncytium and the flooding spread internally to affect the subtegumental tissues. In the tegumental system, there was swelling of the cisternae of granular endoplasmic reticulum and of the mitochondria, with the latter showing signs of breaking down. Autophagic vacuoles and lipid droplets were present and the synthesis of tegumental secretory bodies was much reduced. The gastrodermal cells were severely affected, with swelling and degeneration of the mitochondria and the presence of autophagic vacuoles and lipid droplets. The granular endoplasmic reticulum was swollen and vesiculated and the cells contained few secretory bodies. Both the vitelline and testis follicles showed evidence of extensive cellular disruption and degeneration. This study confirms previous data indicating the potential flukicidal activity of OZ78.

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“…Sections ∼5 m in thickness were stained with haematoxylin and eosin using standard histological protocols, examined and the reproductive tissues photographed using a Leica DM LB2 microscope with a Nikon Coolpix 5000 camera system. For a more detailed protocol, see the descriptions by Hanna et al (2008Hanna et al ( , 2010.…”

Section: Tissue Preparation For Histologymentioning

confidence: 99%

A comparative study on the impact of two artemisinin derivatives, artemether and artesunate, on the female reproductive system of Fasciola hepatica

O'Neill

Johnston

Halferty

et al. 2015

Veterinary Parasitology

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Fasciola hepatica: Histology of the testis in egg-producing adults of several laboratory-maintained isolates of flukes grown to maturity in cattle and sheep and in flukes from naturally infected hosts

Cited by 35 publications

References 10 publications

Fasciola hepatica: disruption of spermatogenesis by the fasciolicide compound alpha

Fasciola hepatica: disruption of spermatogenesis by the fasciolicide compound alpha

Electron microscopical study to assess thein vitroeffects of the synthetic trioxolane OZ78 against the liver fluke,Fasciola hepatica

A comparative study on the impact of two artemisinin derivatives, artemether and artesunate, on the female reproductive system of Fasciola hepatica

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