Fasciola gigantica excretory–secretory products for immunodiagnosis and prevention of sheep fasciolosis

Ridi, Rashika El; Salah, Mohamed; Wagih, A.; William, Hany; Tallima, Hatem; Shafie, Mohamed H. El; Khalek, Tarek Abdel; Amir, Azza El; Ammou, Faten F. Abo; Motawi, H.

doi:10.1016/j.vetpar.2007.08.024

Cited by 27 publications

(17 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The importance of such fact is that clinically it is vital to diagnose fascioliasis as early as possible in serum to avoid all the complications of such a disease. This is in agreement with EL Ridi et al .,[43] who reported the most prominent nine bands of F. gigantica E/S (62-60, 40, 30, and 28 kDa). The data indicated that E/S-based indirect ELISA reached 100% sensitivity and specificity in immunodiagnosis of sheep fascioliasis.…”

Section: Discussionsupporting

confidence: 93%

Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen

et al. 2013

View full text Add to dashboard Cite

Background:Many immunological techniques have been developed over years using the different Fasciola antigens for diagnosis of parasitic infection and to replace the parasitological techniques, which are time consuming and usually lack sensitivity and reproducibility.Materials and Methods:In this study, Fasciola gigantica paramyosin (Pmy) antigen was early detected in cattle sera using sandwich enzyme-linked immunosorbent assay (ELISA), to evaluate the Pmy antigen performance in diagnosis. This work was conducted on 135 cattle blood samples, which were classified according to parasitological investigation into, healthy control (30), fascioliasis (75), and other parasites (30) groups.Results:The sensitivity of Sandwich ELISA was 97.33%, and the specificity was 95%, in comparison with parasitological examination, which recorded 66.66% sensitivity and 100% specificity, respectively.Conclusions:It was clear that the native F. gigantica Pmy is considered as a powerful antigen in early immunodiagnosis of fascioliasis, using a highly sensitive and specific sandwich ELISA technique.

show abstract

Section: Discussionsupporting

confidence: 93%

Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The understanding of the biological, metabolic, biochemical and molecular heterogeneity as well as similarity is very important for the development of proper diagnostic tools to detect the infection at an early stage so that treatment can be administered timely and the prophylactic, control measures may be applied appropriately [ 3 , 51 – 57 ]. An early diagnosis and disease control is very crucial considering the livestock health that is affected by the liver fluke infection not only in India but also across the world, particularly in tropical and sub-tropical countries where enormous morbidity and mortality of the livestock occurs due to fasciolosis.…”

Section: Discussionmentioning

confidence: 99%

Immunolocalization and immunodetection of the excretory/secretory (ES) antigens of Fasciola gigantica

Khan

Rehman

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

The digenetic trematode Fasciola gigantica is a parasite of great agricultural and economic importance. Along with Fasciola hepatica, F. gigantica incurs huge economic losses to the agricultural sector. Because of unavailability of an effective and commercial vaccine, the earliest diagnosis of the disease is the only way to control the disease. The conventional coprological techniques are able to detect the disease only after the parasites get matured and starts releasing their eggs with the faeces of host, therefore prepatent infection remain undiagnosed. The alternative method is by serological tests that uses circulatory antigens. Despite high sensitivity, their reliability is quite low because of the common antigens shared between different helminth parasites. To overcome this, investigation was shifted to identify the copro-antigens which could be more sensitive and reliable. In the present study, we tried to identify some of the immunodominant proteins from the Excretory Secretory (ES) product of F. gigantica which can be further characterized and used for early detection of infection and also as drug and vaccine candidates. The ES products of F. gigantica were collected and used for raising the polyclonal antibody in rabbit. The polypeptide profile was generated as well as immunogenic polypeptides were identified. The Source of ES antigen was immunolocalized using confocal microscopy and dot blot assay was performed to diagnose field infection. The polypeptide profile of ES products revealed a total of 24 polypeptides out of which 12 immunogenic polypeptides were identified by western blotting. Confocal micrographs showed the immunolocalization of antigens in the intestinal caecae, vitalline glands, gonads as well as in the tegument of the worm. The dot blot assay confirmed the utility of ES products for the detection of field infection. Subsequently, cross reactivity was found negative with Gigantocotyle explanatum; an amphitome parasite of same habitat. However, the cross reactivity with other helminths needs to be worked out.

show abstract

“…Therefore, the identification and characterization of these proteins could clarify parasite homeostasis and the host–parasite interaction, which facilitate the development of control strategies against gnathostomiasis, including drugs and vaccines. Furthermore, ESPs can be used for the immunodiagnosis of parasitic infection and for patient monitoring after anthelminthic treatment, as previously demonstrated in several studies of parasitic helminth infection [14, 19, 33, 57].…”

Section: Resultsmentioning

confidence: 99%

Transcriptome and excretory–secretory proteome of infective-stage larvae of the nematode Gnathostoma spinigerum reveal potential immunodiagnostic targets for development

et al. 2019

View full text Add to dashboard Cite

Background: Gnathostoma spinigerum is a harmful parasitic nematode that causes severe morbidity and mortality in humans and animals. Effective drugs and vaccines and reliable diagnostic methods are needed to prevent and control the associated diseases; however, the lack of genome, transcriptome, and proteome databases remains a major limitation. In this study, transcriptomic and secretomic analyses of advanced third-stage larvae of G. spinigerum (aL3Gs) were performed using next-generation sequencing, bioinformatics, and proteomics. Results: An analysis that incorporated transcriptome and bioinformatics data to predict excretory–secretory proteins (ESPs) classified 171 and 292 proteins into classical and non-classical secretory groups, respectively. Proteins with proteolytic (metalloprotease), cell signaling regulatory (i.e., kinases and phosphatase), and metabolic regulatory function (i.e., glucose and lipid metabolism) were significantly upregulated in the transcriptome and secretome. A two-dimensional (2D) immunomic analysis of aL3Gs-ESPs with G. spinigerum-infected human sera and related helminthiases suggested that the serine protease inhibitor (serpin) was a promising antigenic target for the further development of gnathostomiasis immunodiagnostic methods. Conclusions: The transcriptome and excretory–secretory proteome of aL3Gs can facilitate an understanding of the basic molecular biology of the parasite and identifying multiple associated factors, possibly promoting the discovery of novel drugs and vaccines. The 2D-immunomic analysis identified serpin, a protein secreted from aL3Gs, as an interesting candidate for immunodiagnosis that warrants immediate evaluation and validation.

show abstract

Fasciola gigantica excretory–secretory products for immunodiagnosis and prevention of sheep fasciolosis

Cited by 27 publications

References 32 publications

Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen

Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen

Immunolocalization and immunodetection of the excretory/secretory (ES) antigens of Fasciola gigantica

Transcriptome and excretory–secretory proteome of infective-stage larvae of the nematode Gnathostoma spinigerum reveal potential immunodiagnostic targets for development

Contact Info

Product

Resources

About