Fas-Fas ligand system as a possible mediator of spermatogenic cell apoptosis in human maturation-arrested testes

Eguchi, Jiro; Koji, Takehiko; Nomata, Koichiro; Yoshii, Akira; Shin, Masashi; Kanetake, Hiroshi

doi:10.1111/j.1749-0774.2002.tb00100.x

Cited by 22 publications

(17 citation statements)

References 37 publications

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“…Contrarily to previous studies where the FasR/FasL system with caspase-3 activation was suggested to be involved in germ cell degeneration in cases of maturation arrest [7,8,16], our results suggest no active role for apoptosis in this syndrome. On the contrary, another study showed increased immunostaining of active caspase-3 in spermatocytes or spermatocytes and spermatids in cases of incomplete [4] and complete maturation arrest [14].…”

Section: Discussionsupporting

confidence: 76%

Caspase signalling pathways in human spermatogenesis

Almeida

Correia

Rocha

et al. 2013

J Assist Reprod Genet

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Purpose Little is known about the apoptotic mechanisms involved in abnormal spermatogenesis. In order to describe the significance of apoptosis in azoospermia, testicular tissue from abnormal spermatogenesis was analysed. Methods Testicular treatment biopsies were obtained from 27 men. Five presented oligozoospermia, 9 obstructive azoospermia (4 congenital bilateral absence of the vas deferens; 5 secondary azoospermia) and 13 nonobstructive azoospermia (5 hypospermatogenis; 3 maturation arrest; 5 Sertoli-cell-only syndrome). Immunohistochemical staining was performed for active caspases-3, −8 and −9. The presence of active caspases in Sertoli cells and germ cells was analyzed using stereological tools. Results Increased active caspase-3 was found in Sertoli-cellonly syndrome. No significant differences were found in maturation arrest. In hypospermatogenesis, primary spermatocytes were the germ cells with higher active caspases. Oligozoospermia and secondary obstruction showed significant differences among germ cells for the presence of all active caspases. In oligozoospermia, spermatogonia presented significant increased active caspase-9 in relation to active caspase-8. In primary obstruction and hypospermatogenesis, germ cells presented significant increased active caspases-3 and −9. Conclusions Results suggest that increased active caspase-3 might be involved in Sertoli-cell-only syndrome etiology. In cases of hypospermatogenesis, intrinsic lesions at the meiotic stage seem to be related to the pathology. In secondary obstruction apoptosis is suggested to be initiated due to extrinsic and intrinsic lesions, whereas in primary obstruction only the intrinsic apoptotic pathway seems to be present. Finally, in oligozoospermic patients spermatogonia death by mitochondrial damage additionally to meiosis malfunctioning, might be on the origin of the decreased sperm output.

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Section: Discussionsupporting

confidence: 76%

Caspase signalling pathways in human spermatogenesis

Almeida

Correia

Rocha

et al. 2013

J Assist Reprod Genet

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“…This process is very specific and solely restricted to the germ cells of the seminiferous tubules [4,5]. Substantial evidence suggests that apoptosis is under cellular control and involves the Fas/FasL system in testis [6,7].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Fas positive sperm and complement mediated lysis in subfertile individuals

Soleimani

Tavalaee

Aboutorabi

et al. 2010

J Assist Reprod Genet

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“…Testicular disorders and infertility have been associated with deregulation of death cell pathways, which leads to an imbalance between proliferation and death [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Up-regulation of CD95 (Apo-1/Fas) is associated with spermatocyte apoptosis during the first round of spermatogenesis in the rat

et al. 2006

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Apoptosis plays a major role in controlling both the rate of sperm production and chromosomal abnormalities in adult male testes. However, little is known on the mechanisms controlling induction and execution of apoptosis under physiological conditions. In this work we have uncovered a major role for the cell death receptor Fas in both the extrinsic and intrinsic pathways in normal germ cell apoptosis. We show here that Fas levels increased significantly in a group of germ cell in 25 d old rats, which were identified as spermatocytes and only a few spermatogonia. In addition, we show that isolated spermatocytes expressing high levels of Fas display activation of caspase-8, -9, -3, -6 and -2, as well as increased levels of intracellular calcium and decreased pH, which coincides with stabilization of p53, and transcriptional activation of PUMA and Fas. Therefore, our data strongly suggests that transcriptional up regulation of Fas could predispose a group of spermatocytes to Fas ligand triggering apoptosis by the extrinsic and intrinsic pathway.

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Fas-Fas ligand system as a possible mediator of spermatogenic cell apoptosis in human maturation-arrested testes

Cited by 22 publications

References 37 publications

Caspase signalling pathways in human spermatogenesis

Caspase signalling pathways in human spermatogenesis

Evaluation of Fas positive sperm and complement mediated lysis in subfertile individuals

Up-regulation of CD95 (Apo-1/Fas) is associated with spermatocyte apoptosis during the first round of spermatogenesis in the rat

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