Evaluation of Fas positive sperm and complement mediated lysis in subfertile individuals

Soleimani, Maryam; Tavalaee, Marziyeh; Aboutorabi, Roshanak; Adib, Minoo; Bahramian, Hamid; Janzamin, Ehsan; Kiani, Abbas; Nasr-Esfahani, Mohammad Hossein

doi:10.1007/s10815-010-9425-4

Cited by 9 publications

(11 citation statements)

References 25 publications

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“…Our western blot analysis confirmed the presence of Fas and FasL in mouse, rat, bull, ram, and buck spermatozoa, but not in human and boar spermatozoa. Among the 7 species observed in the present study, the presence of Fas receptors in mature spermatozoa has been reported in human (Sakkas et al 1999;McVicar et al 2004;Starace et al 2009;Soleimani et al 2010), bovine ), sheep (Zan Bar et al 2008, and mice (Starace et al 2009), but it has not been observed in goat and rat, to our knowledge. Similarly, the presence of FasL in mature spermatozoa has been reported in human (Riccioli et al 2005), mouse (D'Alessio et al 2001;Riccioli et al 2003), and cattle ), but it has not been observed in pig, sheep, goat, and rat.…”

Section: Discussioncontrasting

confidence: 41%

“…Using a fluorescence-activated Sakkas et al (1999) observed Fas-positivity in less than 10% of the human spermatozoa from normal males and in more than 10% of those from oligozoospermic samples. Similarly, by flow cytometry, Soleimani et al (2010) confirmed the presence of Fas in ejaculated human sperm but concluded that the Fas expression was very low. Thus, it might be the low level of expression in few cells that had led to the conclusion that there was no detectable quantity of Fas on human ejaculated sperm (Perticarari et al 2008).…”

Section: Discussionmentioning

confidence: 89%

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Species difference in the expression of Fas and Fas ligand in mature mammalian spermatozoa

Li¹,

Sun²,

Zhu³

et al. 2017

CZECH J ANIM SCI

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Li Y., Sun M., Zhu J., Jiao G., Lin J., Dong H., Tan J., Zhou J. Although it has been proposed that the Fas and Fas ligand (FasL) may protect ejaculated spermatozoa against apoptosis induced by lipoperoxidative damage and against lymphocytes present in the female genital tract, studies reported conflicting results on the presence of Fas receptors in ejaculated human spermatozoa. Furthermore, the expression of Fas/FasL on mature spermatozoa has not been observed in several important mammals. Using seven species, we observed the possibility for species difference in Fas/FasL expression on mature spermatozoa by both immunofluorescence microscopy and western blot analysis. Whereas intensive signals of Fas immunolabelling were detected in sperm head and middle piece and weak signals observed in the tail in 86-100% of the mouse, rat, bull, ram, and buck spermatozoa, only weak signals were detected on the whole body of 27% boar spermatozoa and in the head of 21% human spermatozoa. The pattern of FasL localization was identical to that of Fas in spermatozoa from human, mouse, rat, ram, and buck, but boar and bull spermatozoa showed weak and intensive FasL signals, respectively, only in the head. Western blotting further confirmed the Fas and FasL expression in mouse, rat, bull, ram, and buck, but not in human and boar spermatozoa. Taken together, the results revealed a marked species difference in Fas/FasL expression and an extensive co-expression of Fas and FasL among mature mammalian spermatozoa, suggesting that whereas spermatozoa from most species may be protected by Fas/FasL, those from human and boar may not use the Fas system for protection.

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Section: Discussioncontrasting

confidence: 41%

Section: Discussionmentioning

confidence: 89%

Species difference in the expression of Fas and Fas ligand in mature mammalian spermatozoa

Li¹,

Sun²,

Zhu³

et al. 2017

CZECH J ANIM SCI

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“…This dysfunctional apoptosis is responsible for presence of defective sperm in fertile men [50][51][52].…”

Section: Aetiology Of Sperm Dna Damagementioning

confidence: 99%

Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility

Shamsi

Imam

Dada

2011

J Assist Reprod Genet

135

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Sperm is not a simple carrier of paternal genetic information but its role extends clearly beyond fertilization. Integrity of sperm genome is an essential pre-requisite for birth of healthy offspring and evaluation of sperm should entail DNA integrity analysis. DNA integrity analysis is a better diagnostic and prognostic marker of sperm reproductive potential. Conventional semen analysis emphasizes on sperm concentration, viability, motility and morphology and has been proven to be a poor indicator of reproductive potential and pregnancy outcome. To overcome the drawbacks associated with conventional semen analysis more useful fertility tests and molecular biomarkers have been explored. Among the different tests which have evolved for assessing the sperm reproductive potential, tests for sperm DNA quality are most promising. Sperm DNA damage has been closely associated with numerous indicators of reproductive health including fertilization, embryo quality, implantation, spontaneous abortion, congenital malformations and childhood diseases. It therefore has great potential as a prognostic test for both in vitro and in vivo conception. This review presents an updated account of tests that have better diagnostic and prognostic implications in the evaluation of sperm DNA damage. The basic principles, outline of methodology, advantage, disadvantage, clinical significance of each technique and implications of these tests have been discussed. The logistics of each test with respect to available resources and equipment in an andrology laboratory, the feasibility of performing these tests in routine diagnostic workup of infertile men and the opportunities and challenges provided by DNA testing in male fertility determination are also presented.

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“…When Fas binds to Fas-L, a molecular complex is formed, signaling initiation of apoptosis, which involves caspase 8 activation and a subsequent cascade of events leading to DNA fragmentation and cell death [68][69][70].…”

Section: Varicocele and Apoptosismentioning

confidence: 99%

“…In the testis, Fas may be found in spermatogenic germ cells, and Fas-L in Sertoli cells [13,69,71]. It seems that the Fas/Fas L system is a major regulator of normal spermatogenesis [72].…”

Section: Varicocele and Apoptosismentioning

confidence: 99%

Immunological Aspects of Varicocele

R¹

2015

MOJI

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Varicocele is the most common cause of infertility in men. The mechanism by which varicocele cause the variable effect on male infertility and is still unknown. Varicocele is found in 35 to 81 % of infertile men, and is one treatable form of male infertility. Although many advances have occurred in the treatment of varicocele, it still represents an important and challenging aspect of basic research (male reproductive physiology and endocrinology, pathophysiology, and pharmacology of reproduction and fertility) and medical practice for urologists, pediatric surgeons, and general physicians, to date. This review provides an overview of the epidemiology, Clinical classification and Immunological aspects of varicocele and discusses the indications for, and interpretation of Immunological aspects of varicocele.

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Evaluation of Fas positive sperm and complement mediated lysis in subfertile individuals

Abstract: Our results have confirmed the presence of selected apoptotic markers such as Fas or phosphatidyl serine on ejaculate sperm, but suggest that Fas expression is low. Further studies are required to investigate the "abortive apoptosis" mechanism through Fas/Fas L.

Cited by 9 publications

References 25 publications

Species difference in the expression of Fas and Fas ligand in mature mammalian spermatozoa

Species difference in the expression of Fas and Fas ligand in mature mammalian spermatozoa

Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility

Immunological Aspects of Varicocele

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