Farnesol Anti-biofilm Activity against Candida albicans Reference and Mutant Strains

Carreiro, Adriana da Fonte Porto; Guedes, Sarah Florindo de Figueiredo; Panariello, Beatriz Helena Dias; Silveira, Paula Ventura da; Duarte, Simone

doi:10.9734/mrji/2017/39345

Cited by 2 publications

(7 citation statements)

References 15 publications

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“…The biosynthesis of exopolysaccharides is accompanied by a decrease in metabolic activity and the transformation of microbial populations to an “uncultivated condition” [ 14 ]. The morphofunctional stability of C. albicans biofilms is based on yeast and hyphal forms, and the process of seedling formation ensures the development of the intercellular matrices [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of farnesol and lyticase on the formation of Candida albicans biofilm

et al. 2020

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Background and Aim: Candida albicans is a dimorphic fungus that has both yeast and filamentous forms. It is part of the normal flora in the oral and genital areas of mammals. One factor for the pathogenicity of C. albicans is its ability to switch from yeast to hyphae. The hyphal form adheres and penetrates tissues more readily than the yeast form and produces biofilms that are associated with chronic infection. Biofilms are protective niches that enable microorganisms to be more resistant to antibiotic treatment, thus allowing for persistent infection. The first stage in the transition from yeast to hyphae involves the formation of a germ tube, and this transition is triggered by interactions with host cells. Germ tube formation is dependent on serum, pH, temperature, and quorum-sensing molecules (QSMs). Farnesol, which is a QSM in C. albicans, can prevent yeast to hyphae conversion and inhibits the growth of fungal biofilm. Lyticase is a synergistic enzyme complex that catalyzes yeast cell lysis by β-1,3-glucanase and is a highly specific alkaline protease that produces protoplasts or spheroplasts. This study investigated the effect of farnesol and lyticase on the formation of C. albicans biofilms. Materials and Methods: C. albicans ATCC 2091 was cultivated on liquid and solid Sabouraud media. The presence of C. albicans was confirmed using HiCrome Candida Agar chromogenic medium. Enzyme activities were assayed using a HiCandida Identification Kit. The morphology and densitometry parameters of C. albicans biofilms were considered in the presence of farnesol (Sigma-Aldrich, Germany), lyticase (from Arthrobacter luteus; Sigma-Aldrich, Germany), and farnesol–lyticase. Results: This study shows that both farnesol and lyticase possess antifungal activity against C. albicans biofilms. A significant difference among treatment groups (p<0.05) was observed from strong biofilm production to medium and weak. Conclusion: Many studies have been devoted to the antimicrobial action of farnesol. Bacterial enzyme lyticase is also used to degrade fungal cell walls. Both molecules show substantial antifungal properties that are similar to the properties of modern antimycotics. The current study demonstrates that farnesol and lyticase can disrupt biofilm formation in C. albicans ATCC 2091, which is an effective biofilm producer.

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Section: Discussionmentioning

confidence: 99%

Effects of farnesol and lyticase on the formation of Candida albicans biofilm

et al. 2020

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“…C. albicans has been detected more frequently in the oral cavities of children with ECC than in children without caries [1,17,18]. C. albicans and Streptococcus mutans are found together and interact within the conducive conditions of ECC [19].…”

Section: Discussionmentioning

confidence: 99%

“…To respond the changes in the biofilm environmental conditions after exposure to ethanol extract of propolis, it is necessary to examine the biomolecular level, including the QS molecule. Farnesol is a QS molecule that acts as an anti-biofilm agent [10,18]. It is produced by C. albicans and blocks the morphological transition from yeast to hyphae [10,18].…”

Section: Discussionmentioning

confidence: 99%

“…Farnesol is a QS molecule that acts as an anti-biofilm agent [10,18]. It is produced by C. albicans and blocks the morphological transition from yeast to hyphae [10,18]. Hyphal development is important for the formation of substantial biofilm biomass [18].…”

Section: Discussionmentioning

confidence: 99%

“…It is produced by C. albicans and blocks the morphological transition from yeast to hyphae [10,18]. Hyphal development is important for the formation of substantial biofilm biomass [18]. In general, farnesol is mostly secreted in the later stages of biofilm formation by C. albicans [10].…”

Section: Discussionmentioning

confidence: 99%

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Biofilm Formation of Candida Albicans Exposed to Ethanol Extract of Propolis

Djais¹,

Jemmy

Putri

et al. 2019

Int J App Pharm

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Objective: Propolis extract showed an excellent in vitro performance against yeast and was additionally found to be fungistatic and fungicidal. Propolisextract is also used for treatment and prevention of fungal infections. However, its effectiveness against Candida albicans biofilm formation requiresinvestigation. The study evaluated the ability of propolis to inhibit C. albicans while the fungus is growing as a biofilm in vitro.Methods: Two reference strains, C. albicans ATCC 25923 and a clinical strain (laboratory stock), were used in this study. For the biofilm experiment,the fungi were cultured in Tryptic Soy Broth medium with 1% sucrose and incubated at 37°C for 24 h, and different concentrations of ethanol extractof propolis were used as the inhibitor agents. Biofilm assays were performed in 96-well microtiter plates, quantification of the total biofilm biomasswas performed using a crystal violet staining method, and the Student’s t-test was chosen for statistical analyses.Results: Our data showed that 3 h incubation with propolis did not affect the biomass in the experimental group compared to the control. When theincubation time was extended to 18 h, the biomass increased significantly compared to the control.Conclusion: This study showed that several concentrations of propolis did not inhibit biofilm. However, in each incubation time, we observed nohyphal morphology in the biofilm mass. Propolis might attenuate the opportunistic virulence of fungus growing as a biofilm in vitro. Further studiesare necessary to confirm this phenomenon.

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Farnesol Anti-biofilm Activity against Candida albicans Reference and Mutant Strains

Cited by 2 publications

References 15 publications

Effects of farnesol and lyticase on the formation of Candida albicans biofilm

Effects of farnesol and lyticase on the formation of Candida albicans biofilm

Biofilm Formation of Candida Albicans Exposed to Ethanol Extract of Propolis

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