Farklı fusarik asit ve bor konsantrasyonları altında in vitro da kültüre alınan patates (Solanum tuberosum cv. Agria) sürgünlerinin gelişimi ve antioksidan tepkileri

Abstract

In this study, the effect of different concentrations of boron (B), fusaric acid (FA), and interaction of B X FA was investigated on the amount of proline and peroxidase enzyme of potato plants (Solanum tuberosum cv. Agria) cultured in vitro. Shoot tips of Agria were cultured into MS medium containing separately B (0, 1, 3, 5, 10 mM), FA (0, 0.1, 0.3, 0.5 mM), different FA X B (0.1 mM FA X 0.1 mM B, 0.1 mM FA X 3 mM B) for a 4 weeks. As a result, shoot length, root length, weight of plant by the 0.1 mM B and 0, 0.1 mM FA were higher than other applications. The application of 3, 5 mM B and 0.3, 0.5 mM FA caused yellowing, root length reductions. It was not observed the root growth the application of different 0.1 mM FA X 1 mM B and 0.1 mM FA X 3 mM B, but the plant length application of 0.1 mM FA X 1 mM B was higher than 0.1 mM FA X 3 mM B. It was determined an increase in proline for amount the application of different FA, B and FA X B. Also, the amount of protein of different concentrations of 0.1, 0.3 and 0.5 mM FA was determined lower than other application and control plants. However, the amount of the enzyme peroxidase (POD) was found lower in the application of 0.3 and 0.5 mM FA than other application and control plant.