To increase the representation and participation of women and other minorities in organizations, workplaces must become more inclusive. For such change to be successful and sustainable, organizations must systematically break down the barriers constraining women's participation and effectiveness; improve their prevailing structures, policies, and practices; and engender transformation in their climates. This article presents the experience of 19 U.S. universities, funded by the National Science Foundation's ADVANCE Institutional Transformation program, that have embraced comprehensive transformation for improved gender representation and inclusion in science and engineering disciplines. It describes the facilitating factors, program initiatives, institutionalization, and outcomes of their transformation, and suggests a transformation model that all organizations can use to create an inclusive and productive workplace for a diverse workforce. © 2008 Wiley Periodicals, Inc.