“…In contrast, the H2N2 and H3N2 viruses that caused pandemics in 1957 and 1968, respectively, were the result of reassortment between avian and human influenza A viruses, acquiring the neuraminidase (NA) and͞or hemagglutinin (HA), and PB1 gene from an avian virus and other genes from the previously circulating human strain (9). However, the earliest human H2 and H3 isolates differed from their avian counterparts in key receptor-binding residues that resulted in preferred binding of the human virus HA to sialic acid (SA) ␣2, 6 receptors, suggesting that a shift from avian virus SA ␣2,3 to human virus SA ␣2,6 receptor-binding specificity is critical for efficient replication and spread of a pandemic strain (10). H5N1 viruses isolated from humans to date possess all eight gene segments that are entirely avian in origin, and with only a few exceptions, have retained a receptor-binding site sequence in the HA that is typical of the avian virus preference for binding SA ␣2, 3 (11-13).…”